LOCUS       sequence-203318-       12336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the expression of NLS-dCas9-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12336)
  AUTHORS   Shao S, Zhang W, Hu H, Xue B, Qin J, Sun C, Sun Y, Wei W, Sun Y
  TITLE     Long-term dual-color tracking of genomic loci by modified sgRNAs of 
            the CRISPR/Cas9 system.
  JOURNAL   Nucleic Acids Res. 2016 May 19;44(9):e86. doi: 10.1093/nar/gkw066. 
            Epub 2016 Feb 4.
  PUBMED    26850639
REFERENCE   2  (bases 1 to 12336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-05-19"; volume: "44(9):e86. doi"; pages: " 
            10.1093/nar/gkw066. Epub 2016 Feb 4"
COMMENT     Sequence Label: PB-CMV-NLS-dCas9-NLS
FEATURES             Location/Qualifiers
     source          1..12336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        284..663
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        664..867
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     818..838
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        912..930
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          1026..1158
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1141..1160
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1188..1197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1203..1223
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1224..1247,1248..1250,1251..3737,3738..3740,
                     3741..5324)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5334..5354
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5427..5492
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5501..5518)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5507..5731
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5863..7041
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6094..7032
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6989..7009
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7049..8080
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFTESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     ELEA"
     CDS             8093..8149
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8774..8884
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     polyA_signal    9019..9140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9056..9075)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9110..9129
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9170..9203
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   9374..9436
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9460..9478)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9499..9515)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9499..9515)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9512..9534)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9523..9539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9547..9553,9554..9571,9572..9577))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9592..9613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9730..9747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9901..10489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9981..10000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10660..11451,11452..11520))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11283..11302
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11521..11625)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11651..12106)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11788..11809)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12000..12019
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12233..12255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12247..12264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12248..12264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12271..12289
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   join(12308..12336,1..6)
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cgcatgcatt cttgaaatat tgctctctct ttctaaatag cgcgaatccg tcgctgtgca
       61 tttaggacat ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca atgcggtaag
      121 tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga ttatctttta
      181 cgtgactttt aagatttaac tcatacgata attatattgt tatttcatgt tctacttacg
      241 tgataactta ttatatatat attttcttgt tatagatatc gttgacattg attattgact
      301 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      361 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      421 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      481 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      541 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      601 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      661 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      721 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      781 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      841 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
      901 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggaattcc atccacgctg
      961 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg aattgcactg
     1021 ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt
     1081 cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact
     1141 ttgcctttct ctccacaggt gtccactccc agttcgctag cttggccgcc accatggcta
     1201 gccccaaaaa gaagaggaaa gtggacaaga agtattctat cggactggcc atcgggacta
     1261 atagcgtcgg gtgggccgtg atcactgacg agtacaaggt gccctctaag aagttcaagg
     1321 tgctcgggaa caccgaccgg cattccatca agaaaaatct gatcggagct ctcctctttg
     1381 attcagggga gaccgctgaa gcaacccgcc tcaagcggac tgctagacgg cggtacacca
     1441 ggaggaagaa ccggatttgt taccttcaag agatattctc caacgaaatg gcaaaggtcg
     1501 acgacagctt cttccatagg ctggaagaat cattcctcgt ggaagaggat aagaagcatg
     1561 aacggcatcc catcttcggt aatatcgtcg acgaggtggc ctatcacgag aaatacccaa
     1621 ccatctacca tcttcgcaaa aagctggtgg actcaaccga caaggcagac ctccggctta
     1681 tctacctggc cctggcccac atgatcaagt tcagaggcca cttcctgatc gagggcgacc
     1741 tcaatcctga caatagcgat gtggataaac tgttcatcca gctggtgcag acttacaacc
     1801 agctctttga agagaacccc atcaatgcaa gcggagtcga tgccaaggcc attctgtcag
     1861 cccggctgtc aaagagccgc agacttgaga atcttatcgc tcagctgccg ggtgaaaaga
     1921 aaaatggact gttcgggaac ctgattgctc tttcacttgg gctgactccc aatttcaagt
     1981 ctaatttcga cctggcagag gatgccaagc tgcaactgtc caaggacacc tatgatgacg
     2041 atctcgacaa cctcctggcc cagatcggtg accaatacgc cgaccttttc cttgctgcta
     2101 agaatctttc tgacgccatc ctgctgtctg acattctccg cgtgaacact gaaatcacca
     2161 aggcccctct ttcagcttca atgattaagc ggtatgatga gcaccaccag gacctgaccc
     2221 tgcttaaggc actcgtccgg cagcagcttc cggagaagta caaggaaatc ttctttgacc
     2281 agtcaaagaa tggatacgcc ggctacatcg acggaggtgc ctcccaagag gaattttata
     2341 agtttatcaa acctatcctt gagaagatgg acggcaccga agagctcctc gtgaaactga
     2401 atcgggagga tctgctgcgg aagcagcgca ctttcgacaa tgggagcatt ccccaccaga
     2461 tccatcttgg ggagcttcac gccatccttc ggcgccaaga ggacttctac ccctttctta
     2521 aggacaacag ggagaagatt gagaaaattc tcactttccg catcccctac tacgtgggac
     2581 ccctcgccag aggaaatagc cggtttgctt ggatgaccag aaagtcagaa gaaactatca
     2641 ctccctggaa cttcgaagag gtggtggaca agggagccag cgctcagtca ttcatcgaac
     2701 ggatgactaa cttcgataag aacctcccca atgagaaggt cctgccgaaa cattccctgc
     2761 tctacgagta ctttaccgtg tacaacgagc tgaccaaggt gaaatatgtc accgaaggga
     2821 tgaggaagcc cgcattcctg tcaggcgaac aaaagaaggc aattgtggac cttctgttca
     2881 agaccaatag aaaggtgacc gtgaagcagc tgaaggagga ctatttcaag aaaattgaat
     2941 gcttcgactc tgtggagatt agcggggtcg aagatcggtt caacgcaagc ctgggtacct
     3001 accatgatct gcttaagatc atcaaggaca aggattttct ggacaatgag gagaacgagg
     3061 acatccttga ggacattgtc ctgactctca ctctgttcga ggaccgggaa atgatcgagg
     3121 agaggcttaa gacctacgcc catctgttcg acgataaagt gatgaagcaa cttaaacgga
     3181 gaagatatac cggatgggga cgccttagcc gcaaactcat caacggaatc cgggacaaac
     3241 agagcggaaa gaccattctt gatttcctta agagcgacgg attcgctaat cgcaacttca
     3301 tgcaacttat ccatgatgat tccctgacct ttaaggagga catccagaag gcccaagtgt
     3361 ctggacaagg tgactcactg cacgagcata tcgcaaatct ggctggttca cccgctatta
     3421 agaagggtat tctccagacc gtgaaagtcg tggacgagct ggtcaaggtg atgggtcgcc
     3481 ataaaccaga gaacattgtc atcgagatgg ccagggaaaa ccagactacc cagaagggac
     3541 agaagaacag cagggagcgg atgaaaagaa ttgaggaagg gattaaggag ctcgggtcac
     3601 agatccttaa agagcacccg gtggaaaaca cccagcttca gaatgagaag ctctatctgt
     3661 actaccttca aaatggacgc gatatgtatg tggaccaaga gcttgatatc aacaggctct
     3721 cagactacga cgtggacgcc atcgtccctc agagcttcct caaagacgac tcaattgaca
     3781 ataaggtgct gactcgctca gacaagaacc ggggaaagtc agataacgtg ccctcagagg
     3841 aagtcgtgaa aaagatgaag aactattggc gccagcttct gaacgcaaag ctgatcactc
     3901 agcggaagtt cgacaatctc actaaggctg agaggggcgg actgagcgaa ctggacaaag
     3961 caggattcat taaacggcaa cttgtggaga ctcggcagat tactaaacat gtcgcccaaa
     4021 tccttgactc acgcatgaat accaagtacg acgaaaacga caaacttatc cgcgaggtga
     4081 aggtgattac cctgaagtcc aagctggtca gcgatttcag aaaggacttt caattctaca
     4141 aagtgcggga gatcaataac tatcatcatg ctcatgacgc atatctgaat gccgtggtgg
     4201 gaaccgccct gatcaagaag tacccaaagc tggaaagcga gttcgtgtac ggagactaca
     4261 aggtctacga cgtgcgcaag atgattgcca aatctgagca ggagatcgga aaggccaccg
     4321 caaagtactt cttctacagc aacatcatga atttcttcaa gaccgaaatc acccttgcaa
     4381 acggtgagat ccggaagagg ccgctcatcg agactaatgg ggagactggc gaaatcgtgt
     4441 gggacaaggg cagagatttc gctaccgtgc gcaaagtgct ttctatgcct caagtgaaca
     4501 tcgtgaagaa aaccgaggtg caaaccggag gcttttctaa ggaatcaatc ctccccaagc
     4561 gcaactccga caagctcatt gcaaggaaga aggattggga ccctaagaag tacggcggat
     4621 tcgattcacc aactgtggct tattctgtcc tggtcgtggc taaggtggaa aaaggaaagt
     4681 ctaagaagct caagagcgtg aaggaactgc tgggtatcac cattatggag cgcagctcct
     4741 tcgagaagaa cccaattgac tttctcgaag ccaaaggtta caaggaagtc aagaaggacc
     4801 ttatcatcaa gctcccaaag tatagcctgt tcgaactgga gaatgggcgg aagcggatgc
     4861 tcgcctccgc tggcgaactt cagaagggta atgagctggc tctcccctcc aagtacgtga
     4921 atttcctcta ccttgcaagc cattacgaga agctgaaggg gagccccgag gacaacgagc
     4981 aaaagcaact gtttgtggag cagcataagc attatctgga cgagatcatt gagcagattt
     5041 ccgagttttc taaacgcgtc attctcgctg atgccaacct cgataaagtc cttagcgcat
     5101 acaataagca cagagacaaa ccaattcggg agcaggctga gaatatcatc cacctgttca
     5161 ccctcaccaa tcttggtgcc cctgccgcat tcaagtactt cgacaccacc atcgaccgga
     5221 aacgctatac ctccaccaaa gaagtgctgg acgccaccct catccaccag agcatcaccg
     5281 gactttacga aactcggatt gacctctcac agctcggagg ggatgaggga gctcccaaga
     5341 aaaagcgcaa ggtaggtagt tccgtgagct aaagctcaac gcgtatcgtc gacaggcgcg
     5401 ccgactaaat cgatggatcc cgggctgact acaaagacca tgacggtgat tataaagatc
     5461 atgacatcga ctacaaggat gacgatgaca agtagtctag cctcgactgt gccttctagt
     5521 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5581 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5641 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5701 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggactagag cgatccctag
     5761 gctaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt
     5821 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     5881 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5941 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6001 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6061 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     6121 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     6181 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagtcc gaggccttgc
     6241 ggttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     6301 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     6361 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     6421 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     6481 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     6541 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     6601 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     6661 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     6721 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     6781 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gaccttttgg
     6841 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     6901 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     6961 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     7021 cttccatttc aggtgtcgtg agtctagaat gaaaaagcct gaactcaccg cgacgtctgt
     7081 cgagaagttt ctgatcgaaa agttcgacag cgtatccgac ctgatgcagc tctcggaggg
     7141 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     7201 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     7261 gctcccgatt ccggaagtgc ttgacattgg ggagttcacg gagagcctga cctattgcat
     7321 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     7381 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     7441 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     7501 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     7561 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     7621 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     7681 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     7741 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     7801 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     7861 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     7921 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     7981 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     8041 acgccccagc actcgtccga gggcaaagga actcgaggct agaggcagcg gcgccacaaa
     8101 cttctctctg ctaaagcaag caggagatgt tgaagaaaac cccgggcctg ctagactgga
     8161 caagagcaaa gtcataaacg gcgctctgga attactcaat ggagtcggta tcgaaggcct
     8221 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
     8281 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
     8341 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
     8401 attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
     8461 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
     8521 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
     8581 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
     8641 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc
     8701 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
     8761 cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
     8821 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
     8881 gctgggatcg ggagagtaag cggccgcgac tctagtcata atcagccata ccacatttgt
     8941 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     9001 gaatgcaatt gttgttgtaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9061 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9121 aaactcatca atgtatctta ggtacaagta ggcggccggc cgcgtcgaga taacttcgta
     9181 taatgtatgc tatacgaagt tatgttttgt tactttatag aagaaatttt gagtttttgt
     9241 ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt
     9301 aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca
     9361 gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat
     9421 gattatcttt ctagggttaa agagctccag cttttgttcc ctttagtgag ggttaatttc
     9481 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9541 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9601 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9661 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9721 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9781 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9841 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9901 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9961 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10021 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10081 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10141 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10201 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10261 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10321 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    10381 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10441 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10501 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10561 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10621 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10681 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10741 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10801 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10861 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10921 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10981 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11041 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    11101 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    11161 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11221 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11281 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11341 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11401 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11461 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11521 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11581 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11641 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
    11701 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
    11761 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
    11821 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
    11881 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
    11941 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
    12001 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
    12061 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
    12121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    12181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    12241 cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt
    12301 accttaaccc tagaaagata gtctgcgtaa aattga
//
