LOCUS       sequence-20332-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   You J, Croyle JL, Nishimura A, Ozato K, Howley PM
  TITLE     Interaction of the bovine papillomavirus E2 protein with Brd4 
            tethers the viral DNA to host mitotic chromosomes.
  JOURNAL   Cell. 2004 Apr 30. 117(3):349-60.
  PUBMED    15109495
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Apr 30. 117(3):349-60."
COMMENT     Sequence Label: p5072 pcDNA4c hBrd4 (1224-1362)
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    104..266
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             279..296
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             300..332
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(336..344,345..359)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttagcgcag aggcttgggg
      121 cagccgagcg gcagccaggc cccggcccgg gcctcggttc cagaagggag aggagcccgc
      181 caaggcgcgc aagagagcgg gctgcctcgc agtccgagcc ggagagggag cgcgagccgc
      241 gccggccccg gacggcctcc gaaaccatgg ggggttctca tcatcatcat catcatggta
      301 tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg
      361 taccaggatc cttcgagcag ttccgccgcg ccgctcggga gaaagaggag cgtgagaagg
      421 ccctgaaggc tcaggccgag cacgctgaga aggagaagga gcggctgcgg caggagcgca
      481 tgaggagccg agaggacgag gatgcgctgg agcaggcccg gcgggcccat gaggaggcac
      541 gtcggcgcca ggagcagcag cagcagcagc gccaggagca acagcagcag cagcaacagc
      601 aagcagctgc ggtggctgcc gccgccaccc cacaggccca gagctcccag ccccagtcca
      661 tgctggacca gcagagggag ttggcccgga agcgggagca ggagcgaaga cgccgggaag
      721 ccatggcagc taccattgac atgaatttcc agagtgatct attgtcaata tttgaagaaa
      781 atcttc
//
