LOCUS       sequence-203338-        1715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes half of a protein delivery sensor ( NLS-FKBP-VN-T2A-RFP) for
            expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Chiu HY, Bates JA, Helma J, Engelke H, Harz H, Bein T, Leonhardt H
  TITLE     Nanoparticle Mediated Delivery and Small Molecule Triggered 
            Activation of Proteins in the Nucleus.
  JOURNAL   Nucleus. 2018 Sep 14. doi: 10.1080/19491034.2018.1523665.
  PUBMED    30217128
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleus. 
            2018 Sep 14. doi: 10.1080/19491034.2018.1523665."
COMMENT     Sequence Label: pCAG-c-Myc NLS-FKBP-Venus N-T2A-mRFP
FEATURES             Location/Qualifiers
     source          1..1715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             81..107
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             114..434
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             114..434
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(489..491,492..950)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(531..552)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(792..811)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             960..1013
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1014..1688
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1014..1688
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1144..1162)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1412..1431
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1606..1629
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcggatcc
       61 accggccggt cgccaccatg cctgctgcta agagagtgaa actggatctc gagggcgtgc
      121 aggtggagac tatctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg
      181 tggtgcacta caccgggatg cttgaagatg gaaagaaatt tgattcctcc cgggacagaa
      241 acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg
      301 ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat tatgcctatg
      361 gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc gatgtggagc
      421 ttctaaaact ggaagtcgac ggaggaggca gcggtggagg tagcggcggt ggaagcggtg
      481 gcggaagcgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      541 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      601 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc
      661 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
      721 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      781 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
      841 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
      901 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc ggcagcggcg
      961 agggcagggg ctccctgctg acctgcggcg atgtcgaaga aaatcccggt cccatggcct
     1021 cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
     1081 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1141 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     1201 ctcagttcca gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     1261 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     1321 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     1381 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     1441 gggaggcctc caccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga
     1501 tgaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacatgg
     1561 ccaagaagcc cgtgcagctg cccggcgcct acaagaccga catcaagctg gacatcacct
     1621 cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc cgccactcca
     1681 ccggcgccgg cctgtacaag taagcggccg cagtt
//
