LOCUS       sequence-20334-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   You J, Croyle JL, Nishimura A, Ozato K, Howley PM
  TITLE     Interaction of the bovine papillomavirus E2 protein with Brd4 
            tethers the viral DNA to host mitotic chromosomes.
  JOURNAL   Cell. 2004 Apr 30. 117(3):349-60.
  PUBMED    15109495
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Apr 30. 117(3):349-60."
COMMENT     Sequence Label: p5077 pcDNA4c hBrd4 (1-594)
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    100..262
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             275..292
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             296..328
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(332..340,341..355)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagctta gcgcagaggc ttggggcagc
      121 cgagcggcag ccaggccccg gcccgggcct cggttccaga agggagagga gcccgccaag
      181 gcgcgcaaga gagcgggctg cctcgcagtc cgagccggag agggagcgcg agccgcgccg
      241 gccccggacg gcctccgaaa ccatgggggg ttctcatcat catcatcatc atggtatggc
      301 tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggtacc
      361 aggatccatg tctgcggaga gcggccctgg gacgagattg agaaatctgc cagtaatggg
      421 ggatggacta gaaacttccc aaatgtctac aacacaggcc caggcccaac cccagccagc
      481 caacgcagcc agcaccaacc ccccgccccc agagacctcc aaccctaaca agcccaagag
      541 gcagaccaac caactgcaat acctgctcag agtggtgctc aagacactat ggaaacacca
      601 gtttgcatgg cctttccagc agcctgtgga tgccgtcaag ctgaacctcc ctgattacta
      661 taagatcatt aaaacgccta tggatatggg aacaataaag aagcgcttgg aaaacaacta
      721 ttactggaat gctcaggaat gtatccagga cttcaacact atgtttacaa attgttacat
      781 ctacaacaag cctggagatg acatagtctt aatggcagaa gctctggaaa agctcttctt
      841 gcaaaaaata aatgagctac ccacaga
//
