LOCUS       sequence-203341-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knock out of EB3 in human cells by CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   van Haren J, Charafeddine RA, Ettinger A, Wang H, Hahn KM, Wittmann 
            T
  TITLE     Local control of intracellular microtubule dynamics by EB1 
            photodissociation.
  JOURNAL   Nat Cell Biol. 2018 Jan 29. pii: 10.1038/s41556-017-0028-5. doi: 
            10.1038/s41556-017-0028-5.
  PUBMED    29379139
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2018 Jan 29. pii: 10.1038/s41556-017-0028-5. doi: 
            10.1038/s41556-017-0028-5."
COMMENT     Sequence Label: pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     104..123
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        203..278
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        375..661
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 acggggatat tcgactgtac accagatatt agtacaaaat acgtgacgta gaaagtaata
       61 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
      121 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
      181 cggtcgccat gatatgcttg cagttttaga gctagaaata gcaagttaaa ataaggctag
      241 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta gagctagaaa
      301 tagcaagtta aaataaggct agtccgtttt tagcgcgtgc gccaattctg cagacaaatg
      361 gctctagagg tacccgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      421 gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg acgtcaatgg
      481 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      541 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct gggcattgtg cccagtacat
      601 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      661 ggtcgaggtg agcccccacg ttctgcttca ctctccccat ctcccccctc tccccacc
//
