LOCUS       sequence-203344-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knock out of EB3 in human cells by CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   van Haren J, Charafeddine RA, Ettinger A, Wang H, Hahn KM, Wittmann 
            T
  TITLE     Local control of intracellular microtubule dynamics by EB1 
            photodissociation.
  JOURNAL   Nat Cell Biol. 2018 Jan 29. pii: 10.1038/s41556-017-0028-5. doi: 
            10.1038/s41556-017-0028-5.
  PUBMED    29379139
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2018 Jan 29. pii: 10.1038/s41556-017-0028-5. doi: 
            10.1038/s41556-017-0028-5."
COMMENT     Sequence Label: pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     106..125
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        205..280
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        377..662
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gatgagatac ttgactgtaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
       61 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
      121 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
      181 accgtcgcca tgatatgctt gcatgtttta gagctagaaa tagcaagtta aaataaggct
      241 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt tagagctaga
      301 aatagcaagt taaaataagg ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa
      361 tggctctaga ggtacccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      421 acgacccccg cccattgacg tcaatagtaa cgccaatagg gactttccat tgacgtcaat
      481 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      541 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattgt gcccagtaca
      601 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      661 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
      721 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
      781 gggggggccc
//
