LOCUS       sequence-20335-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   You J, Croyle JL, Nishimura A, Ozato K, Howley PM
  TITLE     Interaction of the bovine papillomavirus E2 protein with Brd4 
            tethers the viral DNA to host mitotic chromosomes.
  JOURNAL   Cell. 2004 Apr 30. 117(3):349-60.
  PUBMED    15109495
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Apr 30. 117(3):349-60."
COMMENT     Sequence Label: p5078 pcDNA4c hBrd4 (1-470)
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    116..278
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             291..308
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             312..344
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(348..356,357..371)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctggntntta gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag ctggctagcg tttaaactta agcttagcgc
      121 agaggcttgg ggcagccgag cggcagccag gccccggccc gggcctcggt tccagaaggg
      181 agaggagccc gccaaggcgc gcaagagagc gggctgcctc gcagtccgag ccggagaggg
      241 agcgcgagcc gcgccggccc cggacggcct ccgaaaccat ggggggttct catcatcatc
      301 atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg
      361 atgacgataa ggtaccagga tccatgtctg cggagagcgg ccctgggacg agattgagaa
      421 atctgccagt aatgggggat ggactagaaa cttcccaaat gtctacaaca caggcccagg
      481 cccaacccca gccagccaac gcagccagca ccaacccccc gcccccagag acctccaacc
      541 ctaacaagcc caagaggcag accaaccaac tgcaatacct gctcagagtg gtgctcaaga
      601 cactatggaa acaccagttt gcatggcctt tccagcagcc tgtggatgcc gtcaagctga
      661 acctccctga ttactataag atcattaaaa cgcctatgga tatgggaaca ataaagaagc
      721 gcttggaaaa caactattac tggatgctca ggaatgtatc caggacttca acactatgtt
      781 tacaattgtt antctacaac aagcctggna tgantatnt
//
