LOCUS       sequence-203406-        3571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, 
            photoconvertible by primed conversion mechanism, bacterial 
            overexpression, sequence is codon optimised for E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Turkowyd B, Balinovic A, Virant D, Golz Carnero HG, Caldana F, 
            Endesfelder M, Bourgeois D, Endesfelder U
  TITLE     A general mechanism of photoconversion of green-to-red fluorescent 
            proteins based on blue and infrared light reduces phototoxicity in 
            live-cell single-molecule imaging.
  JOURNAL   Angew Chem Int Ed Engl. 2017 Jun 2. doi: 10.1002/anie.201702870.
  PUBMED    28574633
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2017 Jun 2. doi: 10.1002/anie.201702870."
COMMENT     Sequence Label: pRSETa-mKikGR-V69T
FEATURES             Location/Qualifiers
     source          1..3571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..84)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      80..127
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      224..679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(311..330)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     521..542
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        705..809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(810..878,879..1670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1028..1047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1841..2429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2330..2349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2583..2600
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2685..2704
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2685..2703
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2735..2757
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2777..2794
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2798..2830
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(2834..2842,2843..2857)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 taagaattcg aagcttgatc cggctgctaa caaagcccga aaggaagctg agttggctgc
       61 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      121 ttttttgctg aaaggaggaa ctatatccgg atctggcgta atagcgaaga ggcccgcacc
      181 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
      241 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      301 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      361 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      421 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      481 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      541 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
      601 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
      661 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1021 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1321 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2581 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2641 cgcgttggcc gattcattaa tgcaggatct cgatcccgcg aaattaatac gactcactat
     2701 agggagacca caacggtttc cctctagaaa taattttgtt taactttaag aaggagatat
     2761 acatatgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca
     2821 gcaaatgggt cgggatctgt acgacgatga cgataaggat cgatggggat ccatgagcgt
     2881 tatcaccagc gagatgaaga tcgagctgcg catggagggc agcgtgaacg gccacaagtt
     2941 cgtgatcacc ggcaaaggca gtggccgccc ttacgaaggc acccagaccg tggatctgac
     3001 cgtgatcgag ggcggtccgc tgcctttcgc cttcgatatc ctgaccaccg ccttccacta
     3061 cggtaaccgc gtgttcactg agtacccgga ggagatcgtg gactacttca agcagagctt
     3121 tcctgagggc tacagctggg agcgtagcat gagctatgag gacggcggca tctgcctggc
     3181 caccaataac atcaccatga agaaggacgg tagcaatacc ttcgtgaacg agatccgctt
     3241 cgacggcacc aacttccctg caaacggccc ggtgatgcag cgcaagaccg tgaagtggga
     3301 gccgagcacc gagaagatgt acgtgcgtga cggcgttctg aagggcgacg tggagatggc
     3361 cctgctgtta gagggcggtg gccattaccg ctgcgacttc cgcaccacct acaaggccaa
     3421 gaaggtggtg caactgccgg actaccacta tgtggaccac cagatggaga tcaccagcca
     3481 cgacaaggat tacaacaaag tgaaggccta cgagcacgcc aaggcctaca gcggcaccta
     3541 tcgcggcgcc aagtacgaat tcgaggccta a
//
