LOCUS       sequence-20341-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1097
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             110..827
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSVMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMG*TIQM"
     primer_bind     complement(140..168)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     763..783
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttaattaag cccgggctgc agcccggggg atccactagg ttaacgcatg cggcccggta
       61 ccaggcctag atagatagag agagagagag actagtggag gaagaaaaaa tgcgtaaagg
      121 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      181 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      241 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      301 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      361 caagagtgtc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      421 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      481 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      541 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
      601 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
      661 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
      721 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
      781 aacagctgct gggattacac atggcatggg atgaactata caaatgagct ctacaaataa
      841 t
//
