LOCUS       sequence-20343-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1099
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             141..854
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             141..854
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(171..199)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     794..814
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gattaattaa gtcgacatct agagtttaaa ctttaattaa gcccgggctg cagcccgggg
       61 gatccactag gttaacgcat gcggcccggt accaggccta gatagataga gagagagaga
      121 gactagtgga ggaagaaaaa atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      181 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      241 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      301 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      361 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      421 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      481 ttgaaggtgg tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      541 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      601 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      661 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      721 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      781 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      841 atgaactata caaaaggcct gcagcagttt
//
