LOCUS       sequence-20345-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1101
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             142..855
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             142..855
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(172..200)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     795..815
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgattaatta agtcgacatc tagagtttaa actttaatta agcccgggct gcagcccggg
       61 ggatccacta ggttaacgca tgcggcccgg taccaggcct agatagatag agagagagag
      121 agactagtgg aggaagaaaa aatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      181 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      241 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      301 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      361 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      421 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      481 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      541 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      601 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      661 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      721 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      781 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      841 gatgaactat acaaaaggcc tgcagcaaac gacgaaaact acgctgcatc agtttaatga
      901 gctctacaaa taatgaattc cccaggcatc aaataaaacg aaaggctcag tc
//
