LOCUS       sequence-203467-        3338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of FGF4-NanoLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Botero S, Chiaroni-Clarke R, Simon SM
  TITLE     Escherichia coli as a platform for the study of phosphoinositide 
            biology.
  JOURNAL   Sci Adv. 2019 Mar 27;5(3):eaat4872. doi: 10.1126/sciadv.aat4872. 
            eCollection 2019 Mar.
  PUBMED    30944849
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2019-03-27"; volume: "5(3):eaat4872. doi"; pages: " 
            10.1126/sciadv.aat4872. eCollection 2019 Mar"
COMMENT     Sequence Label: pClodANL-FGF4
FEATURES             Location/Qualifiers
     source          1..3338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1017..1039
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(2140..2158)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2226..2330
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2331..2399,2400..3191)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2549..2568)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagatctagc gctgcggaca catacaaagt tacccacaga ttccgtggat aagcagggga
       61 ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg tttttccata ggctccgccc
      121 tcctgccaga gttcacataa acagacgctt ttccggtgca tctgtgggag ccgtgaggct
      181 caaccatgaa tctgacagta cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc
      241 cggcgggtcg ctccctcttg cgctctcctg ttccgaccct gccgtttacc ggatacctgt
      301 tccgcctttc tcccttacgg gaagtgtggc gctttctcat agctcacaca ctggtatctc
      361 ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc aagaactccc cgttcagccc
      421 gactgctgcg ccttatccgg taactgttca cttgagtcca acccggaaaa gcacggtaaa
      481 acgccactgg cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca
      541 cgcggttgct cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg
      601 ctgtgctctg cgaaagccag ttaccacggt taagcagttc cccaactgac ttaaccttcg
      661 atcaaaccac ctccccaggt ggttttttcg aaggttaagt cagttgggga actgcttaac
      721 cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca
      781 gaaaaaaagg atctcaagaa gatcctttga tcagcggccg caaaaaaggt acctgccatg
      841 tatactagac cttaattaac acagctaaca ccacgtcgtc cctatctgct gccctaggtc
      901 tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtataat atattcaggg
      961 agaccacaac ggtttccctc tacaaataat tttgtttaac tttgagggac aataattttg
     1021 tttaacttta agaaggagat atacattact agtatcgatg gttgcactga gcctggcacg
     1081 tctgcctgtg gccgcacaac cgaaagaagc cgccgtgcag agcggtgccg gtgattacct
     1141 gctgggcatt aaacgcctgc gccgcctgta ttgcaacgtg ggcatcggtt tccatctgca
     1201 ggccctgccg gatggtcgca ttggtggtgc ccatgcagat acccgtgata gcctgctgga
     1261 actgagcccg gttgaacgcg gcgtggtgag catctttggt gtggcaagcc gcttctttgt
     1321 ggccatgagt agcaaaggca agctgtatgg cagcccgttt ttcaccgatg aatgcacctt
     1381 taaagaaatt ctgctgccga ataattacaa tgcctatgaa agctacaaat atccgggcat
     1441 gttcatcgcc ctgagcaaaa acggcaagac caaaaaaggc aatcgcgtga gcccgaccat
     1501 gaaagtgaca cactttctgc cgcgcctggg tgcatctaga ggagttttta ctctggaaga
     1561 ttttgttggc gattggcgtc agaccgcggg ttataatttg gatcaagtcc tggaacaggg
     1621 tggcgtaagc tctctgttcc agaacctggg tgtgagcgtg acgccgattc agcgcatcgt
     1681 tctgtccggc gagaacggtc tgaaaattga tattcatgtg atcatcccgt acgaaggcct
     1741 gagcggtgac caaatgggtc aaatcgagaa aatctttaaa gtcgtctacc cagttgacga
     1801 tcaccacttc aaggttatct tgcattacgg tacgctggtg attgatggtg tgaccccgaa
     1861 tatgattgac tatttcggcc gtccgtatga aggcattgcc gtttttgacg gtaaaaagat
     1921 caccgtcacc ggtaccctgt ggaatggcaa taagattatt gacgagcgtc tgattaaccc
     1981 ggacggcagc ctgctgttcc gcgtgaccat caacggtgtc acgggttggc gtctgtgcga
     2041 gcgcatcctg gcataataag tcgacgttaa taacaggcct gctggtaatc gcaggccttt
     2101 ttattttttt ttttgctagc aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     2161 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     2221 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2281 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2341 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2401 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2461 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2521 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     2581 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2641 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2701 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2761 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2821 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     2881 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2941 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3001 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3061 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3121 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3181 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3241 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3301 cttaacgtga gttttcgttc cactgagcgt cagacccc
//
