LOCUS       sequence-203469-        3572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of cherry-NanoLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Botero S, Chiaroni-Clarke R, Simon SM
  TITLE     Escherichia coli as a platform for the study of phosphoinositide 
            biology.
  JOURNAL   Sci Adv. 2019 Mar 27;5(3):eaat4872. doi: 10.1126/sciadv.aat4872. 
            eCollection 2019 Mar.
  PUBMED    30944849
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2019-03-27"; volume: "5(3):eaat4872. doi"; pages: " 
            10.1126/sciadv.aat4872. eCollection 2019 Mar"
COMMENT     Sequence Label: pClodANL-Cherry
FEATURES             Location/Qualifiers
     source          1..3572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1017..1039
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1579..1590
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2374..2392)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2460..2564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2565..2633,2634..3425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2783..2802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagatctagc gctgcggaca catacaaagt tacccacaga ttccgtggat aagcagggga
       61 ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg tttttccata ggctccgccc
      121 tcctgccaga gttcacataa acagacgctt ttccggtgca tctgtgggag ccgtgaggct
      181 caaccatgaa tctgacagta cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc
      241 cggcgggtcg ctccctcttg cgctctcctg ttccgaccct gccgtttacc ggatacctgt
      301 tccgcctttc tcccttacgg gaagtgtggc gctttctcat agctcacaca ctggtatctc
      361 ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc aagaactccc cgttcagccc
      421 gactgctgcg ccttatccgg taactgttca cttgagtcca acccggaaaa gcacggtaaa
      481 acgccactgg cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca
      541 cgcggttgct cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg
      601 ctgtgctctg cgaaagccag ttaccacggt taagcagttc cccaactgac ttaaccttcg
      661 atcaaaccac ctccccaggt ggttttttcg aaggttaagt cagttgggga actgcttaac
      721 cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca
      781 gaaaaaaagg atctcaagaa gatcctttga tcagcggccg caaaaaaggt acctgccatg
      841 tatactagac cttaattaac acagctaaca ccacgtcgtc cctatctgct gccctaggtc
      901 tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtataat atattcaggg
      961 agaccacaac ggtttccctc tacaaataat tttgtttaac tttgagggac aataattttg
     1021 tttaacttta agaaggagat atacattact agtatcgatg tctagagggg tctcaaaggg
     1081 tgaagaggac aacatggcaa taattaaaga gttcatgaga tttaaagtcc atatggaggg
     1141 ttcagtcaac ggacacgagt tcgagattga gggtgaggga gagggaaggc catacgaggg
     1201 aactcagact gcaaagttga aggtcacaaa aggtggacct ttaccattcg catgggacat
     1261 attatcacca cagtttatgt atggttctaa ggcatacgtc aagcacccag ctgatattcc
     1321 agactacttg aagttgtctt ttcctgaagg tttcaaatgg gagagggtca tgaacttcga
     1381 ggacggagga gtcgtcactg tcactcagga ctcatctttg caggatggag agtttatata
     1441 caaagttaaa ttgaggggaa caaacttccc atcagacgga ccagtcatgc aaaagaagac
     1501 tatgggttgg gaggcttctt cagagaggat gtacccagag gacggagctt tgaagggtga
     1561 gataaaacaa aggttgaaat tgaaggacgg tggtcactac gacgctgaag ttaagacaac
     1621 atacaaggct aagaagcctg tccaattgcc tggtgcttat aacgtcaaca ttaaattgga
     1681 cattacttct cacaacgagg attacactat tgtcgagcaa tacgagaggg ctgagggaag
     1741 acactctact ggaggtggca gtgatgggac tagaggagtt tttactctgg aagattttgt
     1801 tggcgattgg cgtcagaccg cgggttataa tttggatcaa gtcctggaac agggtggcgt
     1861 aagctctctg ttccagaacc tgggtgtgag cgtgacgccg attcagcgca tcgttctgtc
     1921 cggcgagaac ggtctgaaaa ttgatattca tgtgatcatc ccgtacgaag gcctgagcgg
     1981 tgaccaaatg ggtcaaatcg agaaaatctt taaagtcgtc tacccagttg acgatcacca
     2041 cttcaaggtt atcttgcatt acggtacgct ggtgattgat ggtgtgaccc cgaatatgat
     2101 tgactatttc ggccgtccgt atgaaggcat tgccgttttt gacggtaaaa agatcaccgt
     2161 caccggtacc ctgtggaatg gcaataagat tattgacgag cgtctgatta acccggacgg
     2221 cagcctgctg ttccgcgtga ccatcaacgg tgtcacgggt tggcgtctgt gcgagcgcat
     2281 cctggcataa taagtcgacg ttaataacag gcctgctggt aatcgcaggc ctttttattt
     2341 ttttttttgc tagcaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     2401 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     2461 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2521 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2581 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2641 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2701 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2761 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     2821 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2881 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2941 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3001 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3061 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     3121 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3181 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3241 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3301 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3361 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3421 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3481 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3541 gtgagttttc gttccactga gcgtcagacc cc
//
