LOCUS       sequence-20348-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1105
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(258..276)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(308..328)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     526..545
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     720..743
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cgacatctag agtttaactt taattaagcc cgggctgcag cccgggggat ccactaggtt
       61 aacgcatgcg gcccggtacc aggcctagat agatagagag agagagagac tagtggagga
      121 agaaaaaatg gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat
      181 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      241 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
      301 ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga
      361 catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
      421 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggctgctt
      481 catctacaag gtgaagttca tcggcgtgaa cttcccctcc gacggccccg taatgcagaa
      541 gaagactatg ggctgggagc cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
      601 gggcgagatc cacaaggccc tgaagttgaa ggacggcggc cactacctgg tggagttcaa
      661 gtccatctac atggccaaga agcccgtgca gctgcccggc tactactacg tggactccaa
      721 gctggacatc acctcccaca acgaggacta caccat
//
