LOCUS       sequence-20349-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1106
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(275..293)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(325..345)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     543..562
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     736..759
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      853..924
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tatcgattaa ttaagtcgac atctagagtt taaactttaa ttaagcccgg gctgcagccc
       61 gggggatcca ctaggttaac gcatgcggcc cggtaccagg cctagataga tagagagaga
      121 gagagactag tggaggaaga aaaaatggcc tcctccgagg acgtcatcaa ggagttcatg
      181 cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      241 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
      301 cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc caaggtgtac
      361 gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga gggcttcaag
      421 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      481 ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg gcgtgaactt cccctccgac
      541 ggccccgtaa tgcagaagaa gactatgggc tgggagccct ccaccgagcg cctgtacccc
      601 cgcgacggcg tgctgaaggc gagatccaca aggccctgaa gctgaaggac ggcggccact
      661 acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact
      721 actacgtgga ctccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc
      781 agtacgagcg cgccgagggc cgccaccacc tgttcctgta ggagctctac aaataatgaa
      841 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      901 tgttgtttgt cggtgaacgc tctcctgagt agga
//
