LOCUS       sequence-203490-       10285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Weber IP, Ramos AP, Strzyz PJ, Leung LC, Young S, Norden C
  TITLE     Mitotic position and morphology of committed precursor cells in the 
            zebrafish retina adapt to architectural changes upon tissue 
            maturation.
  JOURNAL   Cell Rep. 2014 Apr 24;7(2):386-97. doi: 
            10.1016/j.celrep.2014.03.014. Epub 2014 Apr 3.
  PUBMED    24703843
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.03.014"; journalName: "Cell Rep"; date: 
            "2014-04-24- 24"; volume: "7"; issue: "2"; pages: "386-97"
COMMENT     Sequence Label: Cx55.5:rasGFP
FEATURES             Location/Qualifiers
     source          1..10285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      504..513
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(510..512,513..515,516..1226)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(510..512,513..515,516..1226)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(555..576)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(816..835)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1163..1184
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1334..1468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1358..1377)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1412..1431
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(1597..1759,1760..1771)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(1822..1840)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1858..1874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1858..1874)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1871..1893)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1882..1898
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1906..1912,1913..1930,1931..1936))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1951..1972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2089..2106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2260..2848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2340..2359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3019..3810,3811..3879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3642..3661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3880..3984)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4062..4517)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4199..4220)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4643..4665
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4657..4674
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4658..4674
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4681..4699
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(4712..4723,4724..4911)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     promoter        5171..6155
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        6190..6208
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 atatatatat atatatatat atttaacaca gctcagattg cagaaagaat attacaattg
       61 tggttctatt taagtatttt aaaccatgtt agatacacag ctaatatgtg ggtggtgctt
      121 tattttaaaa tggtaattaa taactaattt atctagaaaa atggtggaaa gttttttttt
      181 acatgttggt ctgccattaa tctaaatttt gttggagaga ttgggttttt ttatacttga
      241 ctttcagctt ataaaatgtt ttgaaacata gggttgagac ttttgtctgg atttattgtt
      301 tgcatatttg tgtgaggtta ctgttggaaa acattgattt taaattaaag ctctcaaaca
      361 tttaaataac agttttatac tctggttgat ttaatttcaa tatattttca tatttaaaag
      421 caatttccca tggtctaggt tcatgtttct ttcttcttat tttgtcttta cagacacaaa
      481 aaagcccgag ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
      541 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      601 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      661 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      721 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      781 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      841 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      901 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      961 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1021 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1081 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1141 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1201 ctctcggcat ggacgagctg tacaagggag gaggaagatc taagctgaac cctcctgatg
     1261 agagtggccc cggctgcatg agctgcaagt gtgtgctctc ctgatctaga actatagtga
     1321 gtcgtattac gtagatccag acatgataag atacattgat gagtttggac aaaccacaac
     1381 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     1441 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     1501 ggttcagggg gaggtgtggg aggtttttta attcgcggcc gcgcgcacta gtgaattcca
     1561 tggatatcaa gcttaaacaa gaatctctag ttttctttct tgcttttact tttacttcct
     1621 taatactcaa gtacaatttt aatggagtac ttttttactt ttactcaagt aagattctag
     1681 ccagatactt ttacttttaa ttgagtaaaa ttttccctaa gtacttgtac tttcacttga
     1741 gtaaaatttt tgagtacttt ttacacctct gctcgaccat atgggagagc tcccaacgcg
     1801 ttggatgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt aatcatggtc
     1861 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     1921 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     1981 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2041 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2101 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2161 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2221 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2281 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2341 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2401 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2461 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2521 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2581 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2641 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2701 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2761 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2821 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2881 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2941 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3001 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3061 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3121 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3181 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3241 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3301 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3361 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3421 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3481 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3541 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3601 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3661 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3721 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3781 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3841 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3901 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3961 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg
     4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     4081 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4141 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4201 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4261 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4321 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4381 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4441 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4501 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     4561 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     4621 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
     4681 taatacgact cactataggg cgaattgggc ccagaggtgt aaagtacttg agtaatttta
     4741 cttgattact gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa
     4801 aaatcaatac ttttactttt acttaattac atttttttag aaaaaaaagt actttttact
     4861 ccttacaatt ttatttacag tcaaaaagta cttatttttt ggagatcact tcattctatt
     4921 ttcccttgct attaccaaac caattgaatt gcgctgatgc ccagtttaat ttggcgaacg
     4981 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     5041 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     5101 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     5161 tattacgcca gtcgaccgcc aattcaatat ggcgtatatg gactcatgcc aattcaatat
     5221 ggtggatctg gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat
     5281 ggtggatctg gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat
     5341 ggcggacctg gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag
     5401 gaggggtctt ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc
     5461 ggccatattg gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc
     5521 aatatggcta ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat
     5581 atatgggttt tcctattgac gtagatagcc cctcccaatg ggcggtccca tataccatat
     5641 atggggcttc ctaataccgc ccatagccac tcccccattg acgtcaatgg tctctatata
     5701 tggtctttcc tattgacgtc atatgggcgg tcctattgac gtatatggcg cctcccccat
     5761 tgacgtcaat tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc aataggacca
     5821 cccaccattg acgtcaatgg gatggctcat tgcccattca tatccgttct cacgccccct
     5881 attgacgtca atgacggtaa atggcccact tggcagtaca tcaatatcta ttaatagtaa
     5941 cttggcaagt acattactat tggaagtacg ccagggtaca ttggcagtac tcccattgac
     6001 gtcaatggcg gtaaatggcc cgcgatggct gccaagtaca tccccattga cgtcaatggg
     6061 gaggggcaat gacgcaaatg ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat
     6121 gggaggtcta tataagcaat gctcgtttag ggaaccgcca ttctgcctgg ggacgtcgga
     6181 gcaagcttga tttaggtgac actatagaat acaagctact tgttcttttt gcaggatccg
     6241 ctagcctttc caacagcaca caataataca cttgatatat atggctttga gtaatcaatg
     6301 tacatttctg aaatttacag aaatatccga cccctgatgt gacctgtaaa gggcatttga
     6361 ggatgaaaag agggacttct gctgaccaat cacatctgtt aggtaacagt ctagcagtac
     6421 tttccactac agagcagaat tggggttgtg atttaagtca atgtgtgatg cactaacccc
     6481 aggaataaaa cattaatcta aatttgggct attgatgata attgactgat gtctgggtct
     6541 gtgttttcaa ctaactgtta tttactttag ccatcattgc taacagacaa gtgttgatcg
     6601 gccaccaata ggacatttgc tcctgcaaac agataatttg tctatatcaa tttcagaaat
     6661 gcatctcctt tcgtaatatt tacaaaaatt ttagccctac tgtgaaatta atttattttc
     6721 agatatttcc cacatgctga aatagcatct tcttttttaa caatagtttt aataaataat
     6781 ttcttttgtc tttgctatga taattgcaca ttataccagc tattttgcaa gatattagta
     6841 ttcagtttaa agtgcaattt aaaagcttaa ctagtaggtt aattggagta catataggtt
     6901 tcataatatt aagcactaaa cattagaggt ttatggggcc tgttgaaaat atatttttaa
     6961 ataatatttt acaaacaaac taatagaaat gagaatttct ccagaataat aacataggaa
     7021 atactgttta ataaatgtgt taaacatcac tagggaaata tctgaaaaag aattaaatgt
     7081 ttatctgtgt aaatatttat atactgatgt agcatgacct gtaataattg ttctgtcatg
     7141 ctatatccat acagtgtagg gatgtatgtt gagactacta taaatgttta atcagacgca
     7201 gattaaaatt agtaagtgat cttgcggctc agtaacagag gtcacttcta aatccaacag
     7261 tgtttaaata cataaacatt cccttttatt aatatgtttg tgtaagatca tgatataatt
     7321 atcttaacaa gcataaattg cattcaagct ataatatcct aatatattct aatatcctta
     7381 tattgaatct gtaaaaaaaa tgttaattaa ccctagagtt caagaaaatg agtttttttt
     7441 tggacatttt agttgtttga aataatataa tgcaaaagaa ataatatata aagaaataag
     7501 tctgcagaat aacagattat acacgggtag gttacaaggt tttgtaccat aatatacaac
     7561 tccaacccag acgatttgtc actttaaact attcgaaaaa gtccctttgg gaacttaagg
     7621 ttaaaagttg ttggaagaca catcaaggtc tcataatgca attcaaaagc ataaataaac
     7681 attataaaat cttgaattat tcacagatga ttccttggac ttacaaataa ttttatgtta
     7741 agtggttgta aacaatgtat tttgcctgaa ttaaacaaag caattaagtt gaatattatt
     7801 gcatttaatt agtttgttta agttcaacac acataaattg tttgcaacaa cttttcagat
     7861 attgtttttt cttcagtgta tggtgaactg ctaatttatg gatatttttt aacagtggat
     7921 gcattaaaaa atttttagtt cttcaaatta agtgtcaaag tttgtgtatg atcatgctat
     7981 aattatctca acaaaaataa attgcattta agctatacta tcccacatgc actgtacaaa
     8041 atatctgtta attaacagta ttttgtgatt cacaagtgtt tcccctttat ttatggttgt
     8101 gaattgcatt aatggaactt tgatctctgt cgacttttga tgttgaaaat tcacctacaa
     8161 tttaacaaaa tgacttttat tgacatttta acaaagcata agaaattata tgtagagaaa
     8221 tatatctgta aaataaagga atgcatacgg gcagcttggt tttgtaccat attatacgac
     8281 accatcaacc cggacaattt gtcactttaa actattcgaa atgttagtaa gtcaattttt
     8341 tttaacttga aggataaaag tcaaaaacat caaggtctca taatgcaatt taaaagcatg
     8401 aataatcttt tttacggttg taaattggat tatgggacgt tggtctctgt tctgttgact
     8461 tttgatgttg aactttcaac attacagttt aaagtttaca gtgtcttaaa taacagaaaa
     8521 tgcatggcag ctttttaaac aagctgtttg tactgcaaga tacagcacca accctgacaa
     8581 tatgtcactt taatctattt aaaatgttca taaaagtcac tttgtcaaac atttcaaggt
     8641 taaaagatca agatatgcaa ttcaaaatca aaaataaacg gggaaaaata tcaaataaaa
     8701 acataaatca caaaaaatgg aaaactgcaa atttccggat atatttttac attgtggtct
     8761 gttttgtaga ataccaagct ttaaattaat tcattaaagc atctccagtc taaatctgac
     8821 tatgcagctc atttattata ttaaatatag gacttgacat agcccctaaa tatgtccaag
     8881 ccttgaatga aagcaaccaa ccaacagcat agttagtgag tgtaatgggg ttgcaccatt
     8941 gatttctttt aaatggaata aagtatcacc acaactagca gagacaactt aattacactg
     9001 aatccagcag agtatgaaat cggacatgac cgattgattc ttttctgaac tatcgactgc
     9061 taaaagtagt gccaacatgt ggccaaatgc cattagaaac ttagctttga taataacaac
     9121 tcccttcagc tttctggttt ttaagtatta atcaaattaa atgtgtaaat gttttagttt
     9181 tatgtcttaa aaaaagaacc aataggtctt cagttctgtt gaaaactaat aggcctagtg
     9241 tgtgtgttta tctcaagttg gcacccatgt tgggctagat ttgcagatca gctgaaaaaa
     9301 tcgttctgca atgggtgtta actagcaatc aaccacgcaa gactgtctaa cattacttta
     9361 atcgatagcg ggtgcacaat agcatgagga atcaagtgta cccaagcgta aattcataca
     9421 gcctacagaa gtttttatgt tttattttat cattgcaggg ttttacttac gcatggtata
     9481 cagtggcagt gttagcaagc tgaatcaatt cagttttccc ttcatgttga tagtggagca
     9541 ctgattattg acgctctcac atacagaagc agcgcgcttg ccaacaaagc cctgcgcact
     9601 cggaaacctt cttttggtgc caacaaagag gaaagagggg gtcacaaaag tttaggtgat
     9661 gtgctgcgtc agatgtgcag ggcaccaacg atttttaaat attttggtgg cctcgacgcg
     9721 cctctatgac ccgcggggat ttaaagcagc ggcagactgt ccatactgct gtaaggtaaa
     9781 tgggcacatt gatttctcct gcatgactat aagttggcac ctagacgacc tgcttgttat
     9841 gtgtgtagcc cagaccttta attataagcg ggattgcatg agtctagctt tttgtagcta
     9901 gtgtgtagat gcaggatggg cttgttacaa tgaagattta cttgatttcc ccaacttttg
     9961 tttaaggtac gttgatggtt tatagttgat ttatttgctt tctattaaag ataaagtcgc
    10021 atggagttag aactgtaaac acagcaattg atttcactta ttagcaccac atgcaatgtc
    10081 aattttttgt gacccattaa tagatgttaa ttaagcttgc tgtaattgta tttcagtaca
    10141 aacatgcttg tatttaagga tgtttatgac ctaatttctt ggaacatgtt aaaatatttt
    10201 aacaaatata tatttattat ctttaacgtt atattattat tatagttatt gttattaatt
    10261 tacatattta tctgtgacta cacat
//
