LOCUS       sequence-203497-        3687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Saraiva LR, Ibarra-Soria X, Khan M, Omura M, Scialdone A, Mombaerts 
            P, Marioni JC, Logan DW
  TITLE     Hierarchical deconstruction of mouse olfactory sensory neurons: from
            whole mucosa to single-cell RNA-seq.
  JOURNAL   Sci Rep. 2015 Dec 16;5:18178. doi: 10.1038/srep18178.
  PUBMED    26670777
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-12-16"; volume: "5:18178. doi"; pages: " 
            10.1038/srep18178"
COMMENT     Sequence Label: pISH-Sncg
FEATURES             Location/Qualifiers
     source          1..3687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(852..870)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(853..870)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(888..904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(888..904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(901..923)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    912..928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(936..942,943..960,961..966))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    981..1002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1119..1136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1290..1878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1370..1389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2049..2840,2841..2909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2672..2691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2910..3014)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3092..3547)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3229..3250)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3441..3460
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3673..3687,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3687,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggccgcggga tttcacagca gcatctgatt ggtgatcaga
      121 ggaccatagg gtaaaaggag ccagcagggg gtgctggcgc tggcagtagc gtgtgtggac
      181 ttgtggcact gggctaggga cagagaactt gggcaacctg cagggcactc taggcagggc
      241 attcagtgtg ccaggttgag acagggtcca aggcagaggg gtgtcccttg agcctctgtg
      301 gttggctggc agccttctag tcttctccac tcttggcctc ttcattctcc tcttgctctt
      361 tggcttcttg gtcctgtgca gggggttcca agtcctcctt gcgcaccacc ccggtggtga
      421 ccacgatgtt ttcagcctcc tccacggtct tgttggccac tgtgttgacg ctgctgacca
      481 cagcttcact cacggcattg gcctgctcct tggtcttctc agccactgag gtgacacttt
      541 gtaccacgtt ctccttggtt ttggtgccca catacataac cccctccttg gtcttctcag
      601 ctgcctccgt tactccctgc ttggtctttt ctacagcacc cacaacacct tccttggcaa
      661 tggagaagcc tttcttgaag acgtccatgg tgtttgcaag gatggaacct ggctgctgca
      721 agctatggac tggagtgctg ccgcagccgc tgtctctgct gctactgcct cttattgaat
      781 cactagtgcg gccgcctgca ggtcgaccat atgggagagc tcccaacgcg ttggatgcat
      841 agcttgagta ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt
      901 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      961 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1021 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1081 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1141 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1201 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1261 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1321 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1381 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1441 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1501 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1561 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1621 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1681 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1741 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1801 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1861 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1921 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1981 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2041 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2101 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2161 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2221 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2281 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2341 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2401 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2461 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2521 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2581 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2641 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2701 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2761 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2821 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2881 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2941 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3001 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca
     3061 cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa
     3121 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     3181 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     3241 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     3301 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     3361 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     3421 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     3481 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     3541 ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     3601 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     3661 cgccagggtt ttcccagtca cgacgtt
//
