LOCUS       sequence-203500-        3977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the main olfactory epithelium of
            the mouse.
  JOURNAL   Cell Rep. 2014 Jul 24;8(2):583-95. doi: 
            10.1016/j.celrep.2014.06.010. Epub 2014 Jul 4.
  PUBMED    25001287
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.06.010"; journalName: "Cell Rep"; date: 
            "2014-07-24- 24"; volume: "8"; issue: "2"; pages: "583-95"
COMMENT     Sequence Label: pISH-Taar2
FEATURES             Location/Qualifiers
     source          1..3977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1142..1160)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1143..1160)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1178..1194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1178..1194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1191..1213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1202..1218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1226..1232,1233..1250,1251..1256))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1271..1292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1409..1426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1580..2168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1660..1679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2339..3130,3131..3199))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2962..2981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3200..3304)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3382..3837)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3519..3540)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3731..3750
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3963..3977,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3977,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tatggcatct tttgaagccc
      121 agcaggaaac ctttgattgt tcagagtacg gaaatggatc ttgcccagag aatgaaagat
      181 ctctcggcgt ccgagctgcc atgtactcgt tgatggcctg cgccatattc atcacgatct
      241 ttggcaacct ggccatgatc atttccattt cctacttcaa acagcttcac acaccgacca
      301 acctcctcat tctctccatg gccgtcaccg acttccttct cggattcacc atcatgccat
      361 atagtatggt caggtcagtg gaaaactgct ggtatttcgg acttacgttt tgcaagatcc
      421 attatagctt cgacctgatg cttagcataa cgtccatttt ccacctttgc tcggtggccg
      481 tcgatagatt ttatgccatc tgtcaccctt tgcattattg caccaaaatg actatcccgg
      541 tcgttaggcg gctgctgctc gtctgctggt cagtccccgg ggcgtttgcc tttggggtgg
      601 tcttctccga ggcttacgct gatggaattg aaggctatga cattttggtt gcatgttcca
      661 gttcctgccc agtcatgttc aacaagctat gggggaccac cttgtttgtg gcaggctttt
      721 tcactcctag ctcgatgatg gtggggattt acggcaaaat tttcgctgta tccaaaaaac
      781 acgctcgcgt aattgacaac ttgccagaaa atcaaaacaa tcaaatgagg aaggacaaaa
      841 aagcagccaa aactttaggg atagtgatgg gtgttttctt attgtgttgg tttccatgtt
      901 tcttcaccat cctgttagat ccgtttctga atttctctac gcctgcagtt ctgtttgatg
      961 ccttaacatg gttcggttat tttaattcca catgtaatcc cttaatttat ggtttcttct
     1021 acccttggtt tcgcagagca ctcaagtaca ttttactggg taatcactag tgaattcgcg
     1081 gccgcctgca ggtcgaccat atgggagagc tcccaacgcg ttggatgcat agcttgagta
     1141 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     1201 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1261 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1321 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1381 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1441 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1501 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1561 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1621 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1681 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1741 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1801 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1861 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1921 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1981 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2041 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2101 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2161 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2221 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2281 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2641 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2941 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3301 cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta
     3361 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     3421 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     3481 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     3541 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     3601 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     3661 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     3721 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     3781 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca
     3841 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     3901 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     3961 ttcccagtca cgacgtt
//
