LOCUS       sequence-203501-        4027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the main olfactory epithelium of
            the mouse.
  JOURNAL   Cell Rep. 2014 Jul 24;8(2):583-95. doi: 
            10.1016/j.celrep.2014.06.010. Epub 2014 Jul 4.
  PUBMED    25001287
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.06.010"; journalName: "Cell Rep"; date: 
            "2014-07-24- 24"; volume: "8"; issue: "2"; pages: "583-95"
COMMENT     Sequence Label: pISH-Taar3
FEATURES             Location/Qualifiers
     source          1..4027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1192..1210)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1193..1210)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1228..1244)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1228..1244)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1241..1263)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1252..1268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1276..1282,1283..1300,1301..1306))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1321..1342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1459..1476)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1630..2218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1710..1729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2389..3180,3181..3249))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3012..3031
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3250..3354)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3432..3887)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3569..3590)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3781..3800
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(4013..4027,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4027,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat ttttgcagtg tctgaattag
      121 agcgaaatat ttttcctgac actataaact gaagagcttt ccgaaaccac gggtaaaaaa
      181 agccatggat gagggggttg caagtagagt tgaagtaccc gagccatacc agaagatcca
      241 gcactatgat gggcgtggag tagtctaaat atggatcaat caaaacagcc aggaagcaag
      301 gcagccagca cgctagaaac acccccatga cgatgcccag ggtcttagct gctttcctgt
      361 cctttttctt agacaggttt ttccctactg ctccttttgt gttcgcaggc atatcgctga
      421 gggctcgagc atgtcgtcta gaaacaataa aaattttacc ataaatacca accatgatgg
      481 agccaggagt gaagaagcag gtagtgaaca gtatggtccc ccaaaacttg ttgaacgtaa
      541 gcgcacagaa attgaagcaa gcaacaagaa tctcgtagct ctgcatacca gaaacattgg
      601 cctctgacag aactaaacca aacgaaaaga gagctggggc tgcccagcaa aaagccagca
      661 gtctcttgat catggatacc gtcatggtgg tggtgtagtg caaagggtca cacactgcat
      721 aaaaccggtc gatcgcgatg gagcacaggt ggaatatgga cgtcaggctt agcatcatgt
      781 cgaagctcgc gtggaatttg caaaagctgt ctccgaaata ccagcagctc tccactgatc
      841 tcaccatact gtaaggcatg ataacaaaac ccagcaggaa gtccgtggtt gccatggaga
      901 ggatcagaaa gttggtggga gaatgtagct gtttgaaatg cgatatggaa atgattatca
      961 ctaagtttcc aaagatggtg ataaccatgg ctccagtcat aaacaggtac attatcatgc
     1021 ggacacggaa agagcgattg gtgggagggc aggatttatt tccaaatttt ggacagctgg
     1081 ataagtcttc gggaatgtat attagatcca taatcactag tgaattcgcg gccgcctgca
     1141 ggtcgaccat atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg
     1201 tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1261 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1321 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1381 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1441 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1501 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1561 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1621 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1681 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1741 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1801 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1861 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1921 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1981 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2041 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2101 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2161 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2221 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2281 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2341 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2401 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2461 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2521 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2581 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2641 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2701 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2761 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2821 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2881 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2941 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3001 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3061 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3361 tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     3421 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     3481 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3541 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3601 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3661 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3721 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3781 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3841 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     3901 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     3961 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     4021 cgacgtt
//
