LOCUS       sequence-203515-        8242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  W2.1 plasmid. Contains PTetO-SD8-BE2, PLacO-sgRNA1 and is part of 
            CAMERA 2.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Tang W, Liu DR
  TITLE     Rewritable multi-event analog recording in bacterial and mammalian 
            cells.
  JOURNAL   Science. 2018 Feb 15. pii: science.aap8992. doi: 
            10.1126/science.aap8992.
  PUBMED    29449507
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992."
COMMENT     Sequence Label: pWT037a
FEATURES             Location/Qualifiers
     source          1..8242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..951)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      999..1221
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      1716..1762
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    1796..1814
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1815..1869
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    1820..1838
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    1850..1868
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1931..2617
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(2666..2689,2690..2692,2693..5179,5180..5182,
                     5183..6766)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6779..7033
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     misc_RNA        complement(7147..7222)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7255..7271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    7278..7294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(7352..8143)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(8144..8235)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
     1741 aagactgggc ctttcgtttt attctagagc acagctccat cgaatggcca gatgatccct
     1801 atcagtgata gagattgaca ctctatcatt gatagagtta ttttaccact ccctatcagt
     1861 gatagagaaa agtgaaatga atagttcgac aaaaatctag acctgcaggt gcagtaagga
     1921 ggaaaaaaaa atgtcttctg aaaccggtcc ggttgcggtt gacccgaccc tgcgtcgtcg
     1981 tatcgaaccg cacgaattcg aagttttctt cgacccgcgt gaactgcgta aagaaacctg
     2041 cctgctgtac gaaatcaact ggggtggtcg tcactctatc tggcgtcaca cctctcagaa
     2101 caccaacaaa cacgttgaag ttaacttcat cgaaaaattc accaccgaac gttacttctg
     2161 cccgaacacc cgttgctcta tcacctggtt cctgtcttgg tctccgtgcg gtgaatgctc
     2221 tcgtgcgatc accgaattcc tgtctcgtta cccgcacgtt accctgttca tctacatcgc
     2281 gcgtctgtac caccacgcgg acccgcgtaa ccgtcagggt ctgcgtgacc tgatctcttc
     2341 tggtgttacc atccagatca tgaccgaaca ggaatctggt tactgctggc gtaacttcgt
     2401 taactactct ccgtctaacg aagcgcactg gccgcgttac ccgcacctgt gggttcgtct
     2461 gtacgttctg gaactgtact gcatcatcct gggtctgccg ccgtgcctga acatcctgcg
     2521 tcgtaaacag ccgcagctga ccttcttcac catcgcgctg cagtcttgcc actaccagcg
     2581 tctgccgccg cacatcctgt gggcgaccgg tctgaaatcc ggtagcgaaa caccggggac
     2641 ttcagaatcg gccaccccgg agtctgataa gaaatactca ataggcttag ctatcggcac
     2701 aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa
     2761 ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt
     2821 tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac
     2881 acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt
     2941 agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca
     3001 tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc
     3061 aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt
     3121 aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga
     3181 tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa
     3241 tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc
     3301 tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa
     3361 gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa
     3421 atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga
     3481 tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc
     3541 taagaattta tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac
     3601 taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac
     3661 tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga
     3721 tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag aagaatttta
     3781 taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact
     3841 aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca
     3901 aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt
     3961 aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg
     4021 tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat
     4081 taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga
     4141 acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt
     4201 gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg
     4261 aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt
     4321 caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga
     4381 atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac
     4441 ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga
     4501 agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga
     4561 ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg
     4621 tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa
     4681 gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt
     4741 tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt
     4801 gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat
     4861 taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg
     4921 gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg
     4981 ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag
     5041 tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct
     5101 ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt
     5161 aagtgattat gatgtcgatg ccattgttcc acaaagtttc cttaaagacg attcaataga
     5221 caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga
     5281 agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac
     5341 tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa
     5401 agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca
     5461 aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt
     5521 taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta
     5581 taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt
     5641 tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta
     5701 taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac
     5761 cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc
     5821 aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt
     5881 ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa
     5941 tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa
     6001 aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg
     6061 ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa
     6121 atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc
     6181 ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga
     6241 cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat
     6301 gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt
     6361 gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga
     6421 acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat
     6481 cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc
     6541 atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt
     6601 tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg
     6661 taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac
     6721 tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgacagcg gcggttcaat
     6781 gacaaatctg tcggatatca tcgaaaaaga aaccggtaaa cagctggtta tccaagaaag
     6841 tattttaatg cttcccgaag aagtcgaaga ggtgattggc aacaaacccg aaagtgatat
     6901 tctcgtgcat actgcgtatg atgagtcgac cgatgaaaac gtcatgctgc tgacctccga
     6961 cgcgcccgag tataaaccgt gggctttggt tatccaggat agcaacggtg aaaataagat
     7021 taaaatgtta taaacggagc caatgtacgc aaaaaccccg cttcagcggg gttttttcgc
     7081 caaaaaaaac cccgccctgt caggggcggg gttttttttt atgttgaaaa tctccttcta
     7141 aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac
     7201 ttgctatttc tagctctaaa acccggcaag ctgcccgtgc ccgtgctcag tatcttgtta
     7261 tccgctcaca atgtcaattg ttatccgctc acaattaaat ataatgaccc tcttgataac
     7321 ccaagagggc attttttaat gcccatggcg tttatttgcc gactaccttg gtgatctcgc
     7381 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     7441 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     7501 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     7561 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     7621 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     7681 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     7741 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     7801 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
     7861 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
     7921 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     7981 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
     8041 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
     8101 ctgatagttg agtcgatact tcggcgatca ccgcttccct catactcttc ctttttcaat
     8161 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8221 agaaaaatag gccaaatgcc gt
//
