LOCUS       sequence-203588-         509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  an ampR plasmid built from pBluescript: the multiple cloning site 
            (mcs) flanked by inverted head-to-head oriented I-SceI sites. 
            Genbank accession number is KU144822..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Hoshijima K, Jurynec MJ, Grunwald DJ
  TITLE     Precise Editing of the Zebrafish Genome Made Simple and Efficient.
  JOURNAL   Dev Cell. 2016 Mar 21;36(6):654-67. doi: 
            10.1016/j.devcel.2016.02.015.
  PUBMED    27003937
REFERENCE   2  (bases 1 to 509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2016.02"; journalName: "Dev Cell"; date: 
            "2016-03-21- 21"; volume: "36"; issue: "6"; pages: "654-67"
COMMENT     Sequence Label: pKHR4
FEATURES             Location/Qualifiers
     source          1..509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    338..359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(374..379,380..397,398..404)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    412..428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     417..439
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     436..452
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     436..452
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        473..491
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      241 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      301 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      361 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      421 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc gaaattaacc
      481 ctcactaaag ggaacaaaag ctggtaccg
//
