LOCUS       sequence-203591-       13371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitously expresses a secreted and fluorescently tagged Annexin5 
            for in-vivo apoptosis reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   Hsu AY, Gurol T, Sobreira TJP, Zhang S, Moore N, Cai C, Zhang ZY, 
            Deng Q
  TITLE     Development and Characterization of an Endotoxemia Model in Zebra 
            Fish.
  JOURNAL   Front Immunol. 2018 Mar 29;9:607. doi: 10.3389/fimmu.2018.00607. 
            eCollection 2018.
  PUBMED    29651289
REFERENCE   2  (bases 1 to 13371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2018-03-29"; volume: "9:607. doi"; pages: " 
            10.3389/fimmu.2018.00607. eCollection 2018"
COMMENT     Sequence Label: Tol2-BetaActin-SecA5-YFP
FEATURES             Location/Qualifiers
     source          1..13371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(22..450)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(132..153)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     344..363
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     577..599
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     592..608
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        618..636
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(766..777,778..940)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1312..1332
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6639..6663
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     6665..6681
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6691..6709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7745..7769)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7783..7792
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7789..7791,7792..7794,7795..8508)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7834..7855)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8095..8114)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8442..8463
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(8539..8557)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(8586..8610)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8615..8749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8639..8658)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8693..8712
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(10889..11076,11077..11088)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11122..11138)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11122..11138)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(11175..11193)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11214..11230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11214..11230)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11227..11249)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11238..11254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11262..11268,11269..11286,11287..11292))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11307..11328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11445..11462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11616..12204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11696..11715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12375..13166,13167..13235))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12998..13017
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13236..13340)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
       61 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
      121 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
      181 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
      241 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
      301 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
      361 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
      421 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
      481 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      541 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      601 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtaca ggtcattgca
      661 caacaccaga aatgccctct gatctgcaaa agacgtgaat atctgttcag acacccatat
      721 ccactctgtt ccacacaggt cagaggtttg tccaggagtt cttgacagag gtgtaaaaag
      781 tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt actcaattaa
      841 aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac tccattaaaa
      901 ttgtacttga gtattaagga agtaaaagta aaagcaagaa agaaaactag agattcttgt
      961 ttaagctttt aatctcaaaa aacattaaat gaaatgcata caaggtttta tcctgcttta
     1021 gaactgtttg tatttaatta tcaaactata agacagacaa tctaatgcca gtacacgcta
     1081 ctcaaagttg taaaacctca gatttaactt cagtagaagc tgattctcaa aattgttagt
     1141 gtcaagccta gctcttttgg ggctgaaaag caatcctgca gtgctgaaaa gcctctcaca
     1201 ggcagccgat gcgggaagag gtgtattagt cttgatagag aggctgcaaa tagcaggaaa
     1261 cgtgagcaga gactccctgg tgtctgaaac acaggccaga tgggccgata tcaactttgt
     1321 atagaaaagt tgaaaattcc agtttgaaga aacttttcaa gactcacctt ttcactgttg
     1381 gtcttaattt aggttgatta tttttgtcaa tttaataatt acattgtatt gttcatattt
     1441 tattgtctta ttgtggtgtt tttacagctt ttttattgtt ctgctttgtt actgtttgcc
     1501 ttgtatgcat tgtagcgctt tgggtctgag aaaagcgcac tataaataaa ctgtattatt
     1561 attattaggg atggacatca cggtggcgcg atgggtggca caattacctc acagcaagta
     1621 ggttgctgtt tagagccccg gctggatcag ttggcagttc tgtgtggagt ttgcatgttc
     1681 tccctgttca agtaggtttc ctcccggtgc tctggtttcc cctcacaagt ccaaagacat
     1741 gatgtatagg tgaatggggt aagctaaatt gaccatagtg agtgtgtgtg tgtgtgtgaa
     1801 tgagagtgta taggtgtttc ccagtgttag gttgcaactg gaaggacatc tgctgtgtaa
     1861 aacatatgca ggataagttg gcggttcatt cccctatggt gacccctggt taattaaagg
     1921 actaagttga aaatgaatga atgaatgaat gattagagat aaaccgatac cactttttta
     1981 caaaccgata tgagtacaag tataatagtt tctgttactc gacaatactg agtaccgatt
     2041 ctgatacttc ttggatttgg tttcaatgta attaatttga aagaaggttt tatttcttgc
     2101 ctgtcgcaag gatgaacaca aaaatcttta ccagaaggat attttaagct aagaatatac
     2161 gcacgttgtt gacaaccctg tatcgattac cgattaaacg tggacattta aaggataagt
     2221 tatgcttgcg ccatcacaac tttacacatt acatgatcaa agatcatatt gaatattcgg
     2281 tttattcgtc agaagcctat agatttgtta aaagtgtatt ttataaacgg atggtttata
     2341 aagactaagc tattaatata tcagacattc attaatacat caagacatcg atgtcaggta
     2401 ggacaacaaa tcacatccat gtaatgaaat cttgttgagt ggtattggtg cgtggtttca
     2461 gcatttcatg tgcaatacga gtaagaatat tttaaccaag tatcgaccca aaacctgata
     2521 ctggtatcgg tgcatccctt attattatta ttatcaaaaa cacacttgtt ttatttatgt
     2581 gggtgccaaa caacaaacta tgaaatgcag catgacacgc ccaaaaagtg ttcctttgag
     2641 aacaacttca taaatatgtt tcttatcatt atgcatgaat ggtgtactgg gagagctttg
     2701 gtatcagaat tgagataaga gactttggca ttctaaagga caatgacatt atcacactga
     2761 cagtcaacaa cactttattg ctctggaaat cttatgtcat ggggcaaagc tgaggttcat
     2821 taggacagta ccttatttag agatgaatgg gaaggctgta gtgggctgct atcagggaag
     2881 ggtggtggtc ctcgggggaa ctatggactc acaaaagcac atctgctgta acaagatccc
     2941 ctgtgcaatc tacaaaagtt acaaagagaa gcatggttgg ttaaaaaata gaattgactg
     3001 gatatgctga atgcattgtt tgttgtgtgt tacatcacat taaccacaga ttggtgctaa
     3061 atatttgctt atcttataaa aacctgtgtg tttataaagg ggggtgtgga tggatgcttg
     3121 gcattctttg aaaagatgtt gggggatgga aggaggagac cttattgatg agtatccagt
     3181 ttgcagataa caagttttta gacattcata ctatgatgct gtttatgtct gttttttctc
     3241 attttttcta tacacaaaca gctataaaac attcactttt atatctgatg ctgtatatta
     3301 aatttcgttg tatttattct gtttgctctg ttgggtcata gctagggcca gacagaatct
     3361 gctgacattt tttgcttttt ctgcagagaa ttttgtaaac aatctgtgat ttatgcgaga
     3421 ttttttcggg agtatcgtaa ctaaaaactt actatatgaa ataaaaaaaa ataatacctt
     3481 tttaactttt aataaatata tacaatgcaa gtccacctag atccaattat ttggtcgaca
     3541 aagcaagtct ctcacataat atatcttcta aaagacagaa aacattactt tcagaccata
     3601 ttgtaatcaa atgaacactt tcatattagt caataatatt acggaaatgt atttaaaaac
     3661 tgaataaaca tatatttaca cctttgtctt tatttctaga gtgcttttac aatgtagatt
     3721 gtgtcaaagc agcttaactt agaggattag tatagtaaat tgaaactgtg tcagtccatt
     3781 ttttaagagt ttaagttcag ttcagtttag ttcagtgtga gttaattttt actgctgaga
     3841 gtccaaacac taaagagcaa atccaaatac acaagtcaat aaataaactc aatgatgggc
     3901 taaagatctg gggaaatctg cagatttctg tgcgcacaga ttctgtgtgg gcctagtcat
     3961 tacctatact caacgtttac tctgagaaac ttccatattt tagaggtaaa atgctcagaa
     4021 ttgtaaaaag tcttgtcaaa atacttggaa tttgccaaaa acaaaataaa agtcacatca
     4081 cctcattgtc cttggtatca gcaagcactt ttaacacaat cagctgtttt tgtttataac
     4141 tcccactgaa ggccatgtgc attactccca agttacacag taaaaagtca catgtagttt
     4201 atcatgatca ctgctgctca agcacagctt tatctgttat tcagtgtggc aatatctgaa
     4261 atgtgtgcaa aatattattt attgtagctg atcaccaaca ctttcagtgt tttttttttc
     4321 tacttttaat gagaattatg ttcttaggat ttatccttat aaacctcttt aaaaagaaat
     4381 gttatgtctg catagacacg acatgaaatt atgaacagaa aatttaacta tttaagatca
     4441 agacttgaca tataacattt ctaatgaaca ttaaacctga ggtaggccca cttgcaaaaa
     4501 tgtaactgct atcactcact ttatacatca ttgtgtaact tgtacacaca aaaatagtga
     4561 agcaatgtgg catggaatgc aggtcccatt cattcaaagt tattaaagca ctttcttaaa
     4621 gtgcattact actgagctaa gaccagctag ttgctcatac aacatactac aagtagtgtc
     4681 atcctaattt ggataaaaaa aatgtccatt ttatacatgg aaaagtactc attagatccc
     4741 ccattgtttg tattacggta tttcgtgaac acaagaggta aatgacctac agagctgctg
     4801 ttgtgttaga ttgaaaacac aacacaggat catggaggca tccatcatta tgacgcattc
     4861 tgccacttgt aactctgcac agactttttg aaaagtttaa ttgagtcttg gcgttgtcgc
     4921 agaaaaatac gaaaaattct gtgttaaaat ttgcaaataa aaattccagt tttataggaa
     4981 atcgccatgg ccttgttctt taaccagtta agcttcccct tctttcactc tcaagttgca
     5041 agaagcaagt gtagcaatgt gcacgcgaca gccgggtgtg tgacgctgga ccaatcagag
     5101 cacagagctc cgaaagttta ccttttatgg ctagagccgg gtatgtgccg tcatataaaa
     5161 gagcgcgccc agcttttcag cctcactttg agctcctcca cacgcagcta gtgcggaata
     5221 tcatctgctt gtaacccatt ctcttaagtc gacaaccccc caaacccaag gtgagttggt
     5281 tttacagttt ttaagcgttt aattatagtt tctatctatt aatgttaagt aaaatgtgta
     5341 aatggataaa tatgatttga tgaagtcttt agacagttca taacggtctc tttgtagtta
     5401 tctactaggt ttgatttata agtggtggtt cttttgataa tctatgttac atttaataaa
     5461 tattggctgt atcttaactt ttttgtagcg ttagtggatg tggcaggtga gaatgtcggt
     5521 aatttaacgt gaccagtcgt aggcacgaca ttgaacattg aataggccgg tgtgaaatgg
     5581 gtgttaagtc ttaatataaa gttgtgttct ggctaatctc tctttaacag ccataaatgt
     5641 gatttttaac tacagttttg agggtgctgg tgagcatgtt gcacacttga tggatagccg
     5701 gcatgggaag ttctttgtgc aggcagtgct gcagcagggt gtgacctact ttagctagcc
     5761 ggctaaccag ctctcatctg atgtaacgta aaccccatca atctaacatc ggctcattcg
     5821 tgctttataa gatcgaagat cttgttatta gtaacgggta ttggcattta gttttaactt
     5881 ggttaatttt ctttttgtta tgtgttctat ttaaatgtag cctgcagccc cctctgtctt
     5941 taatgaagct gaagattaac gttaacgtta gtgaagtgtt gtatccgcta tgaaagattt
     6001 gaattttaga ggtgtggtcc actttaataa cggctgcgga gttctctggc tgcacttcag
     6061 ccgctgtgac tgtcgaagca gagcccatca gtttttcagg atctcggtga tttttgaggc
     6121 tagactatga actgaaccga ctgagagtga ccgtccagaa atgctttagt gtgaagtctc
     6181 cgctcacgag gagtccgtgg actgactgca gatctgtgac gcagtaacac ccggcggcag
     6241 acacccgttg aaatgcggtt gtgtaattga tcgcaggcga gggtggaaga ggatgtgaaa
     6301 cttcatttgt gtaaaattta gggagtggtc ctggtgtgat gaatgtcgaa atctgttcct
     6361 ttttactgag ccctacgact ctggctgagt gccacaccgc cggcagccgc aaagcggctc
     6421 aaaccattgc cttttatggt aataatgaga gaatgcagag ggacttcctt tgtctggcat
     6481 atctgaggcg cgcgctgtca ctagcgccca ccagcggtca gactgtagaa tgcagagcaa
     6541 aacaggaagt tgactccaga tggtcacatg cttgctgaaa cgcctctccc tggcagcagt
     6601 gcaattctaa cagtttccct ttcttttaca gttcagcctc aagtttgtac aaaaaagcag
     6661 gcttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
     6721 accgccacca tgcataaggt tttgctggca ctgttcttta tctttctggc accagcaggc
     6781 accatggcac aggttctcag aggcactgtg actgacttcc ctggatttga tgagcgggct
     6841 gatgcagaaa ctcttcggaa ggctatgaaa ggcttgggca cagatgagga gagcatcctg
     6901 actctgttga catcccgaag taatgctcag cgccaggaaa tctctgcagc ttttaagact
     6961 ctgtttggca gggatcttct ggatgacctg aaatcagaac taactggaaa atttgaaaaa
     7021 ttaattgtgg ctctgatgaa accctctcgg ctttatgatg cttatgaact gaaacatgcc
     7081 ttgaagggag ctggaacaaa tgaaaaagta ctgacagaaa ttattgcttc aaggacacct
     7141 gaagaactga gagccatcaa acaagtttat gaagaagaat atggctcaag cctggaagat
     7201 gacgtggtgg gggacacttc agggtactac cagcggatgt tggtggttct ccttcaggct
     7261 aacagagacc ctgatgctgg aatcgatgaa gctcaagttg aacaagatgc tcaggcttta
     7321 tttcaggctg gagaacttaa atgggggaca gatgaagaaa agtttatcac catctttgga
     7381 acacgaagtg tgtctcattt gagaaaggtg tttgacaagt acatgactat atcaggattt
     7441 caaattgagg aaaccattga ccgcgagact tctggcaatt tagagcaact actccttgct
     7501 gttgtgaaat ctattcgaag tatacctgcc taccttgcag agaccctcta ttatgctatg
     7561 aagggagctg ggacagatga tcataccctc atcagagtca tggtttccag gagtgagatt
     7621 gatctgttta acatcaggaa ggagtttagg aagaattttg ccacctctct ttattccatg
     7681 attaagggag atacatctgg ggactataag aaagctcttc tgctgctctg tggagaagat
     7741 gacgacccag ctttcttgta caaagtggtg atatcaccgg tcgccaccat ggtgagcaag
     7801 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     7861 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     7921 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     7981 ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     8041 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     8101 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     8161 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     8221 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     8281 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     8341 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     8401 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     8461 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc ggccgcggtg
     8521 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     8581 atagcaccca gctttcttgt acaaagttga tgtagatcca gacatgataa gatacattga
     8641 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     8701 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     8761 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc
     8821 cgcggcgcca atgcattggg ccctcgagca tatgagatct gtcccgatct ttctcttctg
     8881 tgctgtcaat ttagagtagt ttttgaggta atttgggtgc attctctgta aaaacaacaa
     8941 ataaatattt atattgtatg caataaatgt aacaattaca catacactct ctgattacag
     9001 cattaaaggg ttagttcacc caaaaatgaa aagatgtcat taatgactca ccctcatgtc
     9061 gttccaagcc cgtaacacct ccgttcatct tcggaacaca gtttaagata ttttagattt
     9121 agtccgagag ctttctgtcc ctccactgaa aatgtatgta cggtatactg tccatgtcca
     9181 gaaaggtaat aaaaacatca aagtagtcca tgtgacatca gtgggttagg tagaattatt
     9241 tgaagcatcg aaaatacatt ttggtccaaa aataacaaaa cctacgactt tattcagcat
     9301 tgtagcgtca ctgcgaagtc gtgaacgcgg attgacaaca gacccggaag agaatacaat
     9361 gccgaataaa gtcgtaggtt ttgttatttt tggaccaaaa tgtattcgat gcttcaaaaa
     9421 attctaacta acccactgat gtcacatgga ctactttgat gtttttatta cctttctgga
     9481 catggacagt ataccgtaca tacattctca atggaggcac agaaagctct cggactaaat
     9541 ctaaaatatc ttaaactgtg ttctgaagat gaacggaggt cttacgggct tggaacgaca
     9601 tgagggcgag tcattaatga cattattttc atttttgggt gaactaaccc tttaataaaa
     9661 ctgacatttg cagccaattg cacactttat aatgttaaat acttgttgat tttagaaata
     9721 cagtagcatt tagtgacaat caaacattaa ttttatttct gattatattg acaaaaacac
     9781 aggactgtaa ggctgacatg acagggtgaa ttgatttcat tacattagtt ttgatggtaa
     9841 cacttttaca ataaggttcg ttagttaact acattagtta acatgaacta ataatgaact
     9901 gcacgtatac atgttaattt caacatttac taatacttta tatcttgtta acattagtta
     9961 atgcactgtg aactaacatg aacaaacaat gaacatctgt atttctatta actaacgtta
    10021 acaaagatta acagatacag taacaaatgt attgctcatg gttatttaat gttagttaat
    10081 acatgaacta atcaacctca ttgtaaagtg ttaccgtttt gacaatacaa catgtttgga
    10141 aaaaaatttc tcgcttggtt ttcttgtgtt ttgcatgaat tcaaacacaa tgagagcacg
    10201 gaatgataat gattattcaa agctgcaata ggcgccaaat ttcccgtgga cctaacgtta
    10261 ccaattacaa atgcatttta gcattttatt acgtgagcct tatattgtca atcgcatgca
    10321 ttagtgaaaa taggcctact acacatataa aaggcgcgcc atcaacgtca aaacatgcac
    10381 acccaaaaaa aaaaaaaaaa aaaaaaaaaa aactatcagt aaaacaaaat acttaatgta
    10441 cttacctcaa tatgcttcct taggtttgat ggcgaacttt tgaaggccga tatttcttta
    10501 ttaagcggga gacagaggac acatttcatc ttgaatgaat ctttatttac accacttaaa
    10561 gaaaagaatt cgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact
    10621 gtgctgctcg cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact
    10681 gttataattt ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat
    10741 gacgtcactt ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga
    10801 ttttcatagg cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg
    10861 tttggtaata gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac
    10921 tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta
    10981 aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata
    11041 cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc
    11101 ctaccttcag caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc
    11161 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
    11221 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    11281 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    11341 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    11401 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    11461 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    11521 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    11581 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    11641 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    11701 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    11761 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    11821 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    11881 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    11941 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    12001 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    12061 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    12121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    12181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    12241 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    12301 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    12361 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    12421 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    12481 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    12541 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    12601 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    12661 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    12721 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    12781 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    12841 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    12901 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    12961 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    13021 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    13081 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    13141 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    13201 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    13261 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    13321 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt g
//
