LOCUS       sequence-203596-       10320 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  heat shock inducible transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10320)
  AUTHORS   Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C
  TITLE     Interkinetic nuclear migration is centrosome independent and ensures
            apical cell division to maintain tissue integrity.
  JOURNAL   Dev Cell. 2015 Jan 26;32(2):203-19. doi: 
            10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15.
  PUBMED    25600237
REFERENCE   2  (bases 1 to 10320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.12.001"; journalName: "Dev Cell"; date: 
            "2015-01-26- 26"; volume: "32"; issue: "2"; pages: "203-19"
COMMENT     Sequence Label: HS-aPKC-CAAX-mKate2
FEATURES             Location/Qualifiers
     source          1..10320
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(1076..1087,1088..1250)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1623..1639
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1659..1679
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3199..3223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3224..3919
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(3921..3945)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5790..5810)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5818..5834)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5850..5984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5874..5893)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5928..5947
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6423..6610,6611..6622)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6656..6672)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6656..6672)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6709..6729)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6709..6727)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6748..6764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6761..6783)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6772..6788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6796..6802,6803..6820,6821..6826))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6841..6862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6979..6996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7150..7738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7230..7249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7909..8700,8701..8769))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8532..8551
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8770..8874)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8900..9355)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9037..9058)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9249..9268
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9482..9504
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9497..9513
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9523..9542
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9523..9541
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
       61 gattgattga ttgattgatt ggtagtcaaa ataagaaata atttccacag attcattaca
      121 gaaatgatta aatgcataca taaaaaactg gggggggggn gggatacaac aacacactta
      181 agtcacattt gcctacgtaa agaaaagtaa agaaaatcaa tagctatatt ttacatctcc
      241 tttttttgct gtcttttaat tagccttgtt ttgctgttat cttgattgaa actgtaactt
      301 cttcacctgc ttcttttctt tgtaggtttt gccagcccag aaacaggcat ggctgtgcaa
      361 ggccagagca ccatgcacaa tgcgctgcat gtcttcatga acggctcaat gtcctcagtc
      421 cagggctcag ccaacgaccc catcttcctc cttcaccatg ctttcattga caggtaacaa
      481 acacgtcatg acattagact gcacagtttt tgacaaagtt catacaatct gttgtttata
      541 gctgctacaa ttagtgaagt ttgtgaatgt acttggatga gcagcgaaag atcaattgag
      601 atcaattgtt agagtttggt tgccctgcag agcaaagaac aaaaaataat ctggtggctt
      661 tactgcgtga ggttattatt ggtggaatag aaacacaaaa cataattgca tttatttgtt
      721 taatttttta tcttatctta actttcatct tgcatatttg tttcttacat catttctagc
      781 atctttgagc gctggctaag aactcatcag cctccccggt ccatctaccc acgtaccaat
      841 gcaccaattg gccacaatga cggctactac atggtgccat tccttcctct ttataggaat
      901 ggagactacc tcctgtccaa caatgctctt ggatacgagt acgcctacct gttggaccca
      961 ggtcattgca caacaccaga aatgccctct gatctgcaaa agacgtgaat atctgttcag
     1021 acacccatat ccactctgtt ccacacaggt cagaggtttg tccaggagtt cttgacagag
     1081 gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt
     1141 actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac
     1201 tccattaaaa ttgtacttga gtattaagga agtaaaagta aaagcaagaa agaaaactag
     1261 agattcttgt ttaagctttt aatctcaaaa aacattaaat gaaatgcata caaggtttta
     1321 tcctgcttta gaactgtttg tatttaatta tcaaactata agacagacaa tctaatgcca
     1381 gtacacgcta ctcaaagttg taaaacctca gatttaactt cagtagaagc tgattctcaa
     1441 aattgttagt gtcaagccta gctcttttgg ggctgaaaag caatcctgca gtgctgaaaa
     1501 gcctctcaca ggcagccgat gcgggaagag gtgtattagt cttgatagag aggctgcaaa
     1561 tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac acaggccaga tgggccctcg
     1621 agcaggaaac agctatgacc atgattacgc caagctatca actttgtata gaaaagttgc
     1681 ttcaggggtg tcgcttggtt atttccaaaa atcaaattaa ttttattaaa ctattagaac
     1741 gagcatgttt tgtctatatg ctacagaaga taaaaaataa taggagttaa cagttataaa
     1801 acaacacact ttgtttctat tgattgttga ccacactggg gtctcattaa gttagattaa
     1861 agacacacta actgggtcaa aagcagcaga ttgatttcat agcaccaggg taaactttct
     1921 aacactttta cggcaatcat atacattaaa attaaataca gaccacgact gaacaaggag
     1981 gatgatctcc aatattaaac aaagagactt gtgcctattt ctctgagggt aaacatgacc
     2041 tctcaagtta gcaagttggt tttaacacta caaaaatagt taagactgca atcccagaat
     2101 aaagtattgg ttttaaccaa tcaatatagt acagtaaaca tccatttgtt ttgttgaaac
     2161 gttaaacaaa tctgaccaaa gctattagct tatataaaac aggtttgcct tctatgtagc
     2221 tgaaaacacc acaggcccga ttttgctact gtgtaaaaca tttcagcaag atttttttat
     2281 tgcatttttt tttactgaat cgttcaaaca ttttatcatt ttagtttgtt cattcattgc
     2341 aactggaaaa acaacacatc acacaaccgc acatatttca gcaataagta caataaaaca
     2401 ctcaaataaa aaaaacattt taaatctctt tgtatttttg accgctgttt cgcgtaattt
     2461 cacggtaaaa ctctggaaat ctccactaca ttcctctcag cggctcctct caatgacagc
     2521 tgaagaagtg acgcggctgc ctgctgtgtt ttgattggtc gaattcactg gaggcttcca
     2581 gaacagtgta gagtctgaac gggtgcgcgc tctgctgtat ttaaagggcg aaagagagac
     2641 cgcagagaaa ctcaaccgaa gagaagcgac ttgacaaaga agaaaagagc agcctgacag
     2701 gactttttcc ccgacgaggt gtttattcgc tctatttaag aatctactgt aaggtaagtc
     2761 tcaatatatt gtactctatt ggctaatcag aattatatag agattatatg tacttaatgt
     2821 caaaaaatca actttgtata tgtaatcttt ttacatgtgg actgcctatg ttcatcttat
     2881 tttaggtcta ctagaaaatt atatttcccg ttttcacaat aaggattttt aaaaaaagca
     2941 atgaacagac gggcatttac tttatgttgc tgacattatt ttatatgagc ataataacca
     3001 taaatactag caaatgtcct aaatgaattt gtgttaatgt tgtctacaaa agaaaattag
     3061 cgttttactt gtacaactaa taataacttg gttattaaga gaatttcact tgttgactag
     3121 aaaaatcctt tcataatgaa acaattgcac cataaattgt ataaatataa aattaattct
     3181 aattgttttt ttttcctgcc aagtttgtac aaaaaagcag gctatggtga gcgagctgat
     3241 taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
     3301 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggc
     3361 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
     3421 cagcaaaacc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
     3481 cgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     3541 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     3601 cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg cctccaccga
     3661 gaccctgtac cccgctgacg gcggcctgga aggcagagcc gacatggccc tgaagctcgt
     3721 gggcgggggc cacctgatct gcaacttgaa gaccacatac agatccaaga aacccgctaa
     3781 gaacctcaag atgcccggcg tctactatgt ggacagaaga ctggaaagaa tcaaggaggc
     3841 cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
     3901 tagcaaactg gggcacagaa acccagcttt cttgtacaaa gtggctatgc ccagcaggac
     3961 ggaccccaag atggaccgga gcggcggccg cgtccgtctg aaggcgcact acggcgggga
     4021 catcctgatt accagcgtgg atgccatgac aacattcaag gacctctgtg aggaagtgcg
     4081 agacatgtgt ggcctgcacc agcagcaccc actcaccctc aagtgggtgg acagtgaagg
     4141 tgacccttgt actgtgtcct cacagatgga gctggaggcc ttccgcctgg cctgtcaggg
     4201 cagggacgaa gtgctcatca tccacgtttt cccaagcatc ccagaacaac cgggcatgcc
     4261 ttgtcctgga gaagacaagt ccatctaccg ccgtggagcc agaagatgga ggaagctata
     4321 ccgagccaac ggccacctct tccaagccaa gcgctttaac aggagagcgt actgtggcca
     4381 gtgcagcgaa aggatatggg gcctcgcgag gcaggggtac aggtgcatca actgcaagct
     4441 gcttgtccat aaacgctgcc acgtcctcgt cccgctgacc tgcaggaggc atatggattc
     4501 tgtcatgcct tcccaagagc ctccagtaga tgacaagaac gatggtgtag accttccttc
     4561 agaagaaact gatggaattg cttatatttc ttcatctcgg aaacatgaca atatcaaaga
     4621 tgattctgag gaccttaagc ctgtcatcga tggggtggat gggatcaaaa tctctcaggg
     4681 gctggggctg caagactttg acctcatcag agtcatcggg cgtggaagct atgccaaggt
     4741 cctcctggtg cggttgaaga aaaacgacca gatttacgcc atgaaggtgg tgaagaagga
     4801 gctcgtccac gacgatgagg atatcgactg ggtgcagaca gagaagcacg tgttcgagca
     4861 ggcatccagc aaccccttcc tggttggctt acactcctgc ttccagacaa cgagccggtt
     4921 gttcctggtc atcgagtatg tcaacggggg ggacctcatg ttccacatgc agaggcagag
     4981 gaagcttcca gaggaacacg ccaggttcta tgctgctgag atctgtatcg ctctcaactt
     5041 cctacatgag agagggatca tctaccggga cctaaaactg gacaacgtcc tcctcgatgc
     5101 cgatggacac attaagctga cggactacgg catgtgcaag gaaggcctag gccccggcga
     5161 cacaacaagc actttttgtg gaaccccgaa ctatatcgcc cccgaaatcc tgcgaggaga
     5221 agagtacggg ttcagcgtgg actggtgggc gctgggtgtc cttatgtttg agatgatggc
     5281 tgggcgctcc ccctttgaca tcatcacaga caaccctgac atgaatactg aagactacct
     5341 tttccaagtt atcctggaaa agccaattcg gattccccgt ttcctgtctg tcaaggcctc
     5401 acacgtcttg aaaggatttt taaataagga tcccaaagag aggcttggct gccggccgca
     5461 gactgggttt tccgacatca agtcccatgc cttcttccga agcatagact gggacctgct
     5521 tgaaaagaag cagaccctgc ctcccttcca gccccagatc acagatgact atggcctgga
     5581 caacttcgac acgcagttca ccagcgagcc cgtacagctg accccagatg atgaggacgt
     5641 cataaagagg atcgaccagt ccgagttcga aggcttcgag tacatcaacc cgcttctgct
     5701 gtctgctgag gagtccgtgc tcgagaagct gaaccctcct gatgagagtg gccccggctg
     5761 catgagctgc aagtgtgtgc tctcctaagc aactttatta tacatagttg ataattcact
     5821 ggccgtcgtt ttacggtacc atcgatgatg atccagacat gataagatac attgatgagt
     5881 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     5941 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     6001 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     6061 tctacaaatg tggtatggct gattatgatc ctctagatca gatctgcgaa gatacggcca
     6121 cgggtgctct tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc
     6181 acatacttcc tccattttct tccactgatt gactgttata atttccctaa tttccaggtc
     6241 aaggtgctgt gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa
     6301 catgtctgac caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt
     6361 taaattaaac tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa
     6421 tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa
     6481 agtacttttt tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg
     6541 tactcaagta aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt
     6601 actcaagtac tttacacctc tggttcttga ccccctacct tcagcaagcc cagcagatcc
     6661 actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg
     6721 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6781 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6841 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6901 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6961 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7021 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7081 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7141 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7201 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7261 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7321 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7381 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7441 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7501 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7561 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7621 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7681 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7741 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7801 cattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7861 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7921 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7981 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8041 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8101 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8161 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8221 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8281 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8341 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8401 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8461 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8521 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8581 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8641 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8701 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8761 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8821 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8881 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     8941 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     9001 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     9061 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     9121 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     9181 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     9241 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     9301 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     9361 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     9421 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     9481 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     9541 ggcgaattgg gtaccaaata gtaggaatta cccacctgta caagtgctga aaacttggat
     9601 gaataagccc gtttgccatt ttctactgct attttaaatc ttttctgtat ttgtctgcat
     9661 ttgtctttac ccttgaataa atgttttagt tgtttttttt caattatgac tgtgtttaac
     9721 agacattatt acaataatat gtaatagtag tacacactat tattatgtaa tagtacttgt
     9781 tgactgtatt tgagaactgg accgagtgag tgttacgtca cccattcaaa atgacttact
     9841 tctggctcca acaaaatgaa gttaattcag ttgccatttt tcactgtatg gacatcgccg
     9901 tgttggagct agacgttgcc aataagcaag aaagagccga gatgcgtcga gtctgagtca
     9961 ccgttcctat ggcaacccct ctaaccaatc agaagtaagc ttgttggaag tccacagcct
    10021 accacttgaa agcgggctgc acaaaatctg tcaaacgttt tgaacgttgg atgtgagagc
    10081 acatactttt attaaggcat ctggttggtc agtttataat atcaacaact tgggctacag
    10141 aaaagaaaag ttattacaga aattatgatt aacaagtaca tgttaaataa agattttaat
    10201 atgaatgcca ccactggagc attcatgcca tttggagctt cttcctgttt ggatcactag
    10261 aaggaggagg tcactcatta cagttctcat atacagtcgt tggttggttg gttggttggt
//
