LOCUS       sequence-203597-       10353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock inducible transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C
  TITLE     Interkinetic nuclear migration is centrosome independent and ensures
            apical cell division to maintain tissue integrity.
  JOURNAL   Dev Cell. 2015 Jan 26;32(2):203-19. doi: 
            10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15.
  PUBMED    25600237
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.12.001"; journalName: "Dev Cell"; date: 
            "2015-01-26- 26"; volume: "32"; issue: "2"; pages: "203-19"
COMMENT     Sequence Label: HS-aPKC-CAAX-mKate2
FEATURES             Location/Qualifiers
     source          1..10353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2536..2547,2548..2710)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     3083..3099
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3119..3139
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4659..4683
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4684..5379
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(5381..5405)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7250..7270)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7278..7294)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7310..7444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7334..7353)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7388..7407
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7883..8070,8071..8082)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8116..8132)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8116..8132)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8169..8189)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8169..8187)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8208..8224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8221..8243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8232..8248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8256..8262,8263..8280,8281..8286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8301..8322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8439..8456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8610..9198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8690..8709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9369..10160,10161..10229))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9992..10011
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10230..10334)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgttgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggttgg ttggtagatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattgattga ttgattgatt gattgattga ttgattgatt ggtagtcaaa ataagaaata
     1561 atttccacag attcattaca gaaatgatta aatgcataca taaaaaactg gggggggggn
     1621 gggatacaac aacacactta agtcacattt gcctacgtaa agaaaagtaa agaaaatcaa
     1681 tagctatatt ttacatctcc tttttttgct gtcttttaat tagccttgtt ttgctgttat
     1741 cttgattgaa actgtaactt cttcacctgc ttcttttctt tgtaggtttt gccagcccag
     1801 aaacaggcat ggctgtgcaa ggccagagca ccatgcacaa tgcgctgcat gtcttcatga
     1861 acggctcaat gtcctcagtc cagggctcag ccaacgaccc catcttcctc cttcaccatg
     1921 ctttcattga caggtaacaa acacgtcatg acattagact gcacagtttt tgacaaagtt
     1981 catacaatct gttgtttata gctgctacaa ttagtgaagt ttgtgaatgt acttggatga
     2041 gcagcgaaag atcaattgag atcaattgtt agagtttggt tgccctgcag agcaaagaac
     2101 aaaaaataat ctggtggctt tactgcgtga ggttattatt ggtggaatag aaacacaaaa
     2161 cataattgca tttatttgtt taatttttta tcttatctta actttcatct tgcatatttg
     2221 tttcttacat catttctagc atctttgagc gctggctaag aactcatcag cctccccggt
     2281 ccatctaccc acgtaccaat gcaccaattg gccacaatga cggctactac atggtgccat
     2341 tccttcctct ttataggaat ggagactacc tcctgtccaa caatgctctt ggatacgagt
     2401 acgcctacct gttggaccca ggtcattgca caacaccaga aatgccctct gatctgcaaa
     2461 agacgtgaat atctgttcag acacccatat ccactctgtt ccacacaggt cagaggtttg
     2521 tccaggagtt cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac
     2581 aagtacttag ggaaaatttt actcaattaa aagtaaaagt atctggctag aatcttactt
     2641 gagtaaaagt aaaaaagtac tccattaaaa ttgtacttga gtattaagga agtaaaagta
     2701 aaagcaagaa agaaaactag agattcttgt ttaagctttt aatctcaaaa aacattaaat
     2761 gaaatgcata caaggtttta tcctgcttta gaactgtttg tatttaatta tcaaactata
     2821 agacagacaa tctaatgcca gtacacgcta ctcaaagttg taaaacctca gatttaactt
     2881 cagtagaagc tgattctcaa aattgttagt gtcaagccta gctcttttgg ggctgaaaag
     2941 caatcctgca gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag gtgtattagt
     3001 cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac
     3061 acaggccaga tgggccctcg agcaggaaac agctatgacc atgattacgc caagctatca
     3121 actttgtata gaaaagttgc ttcaggggtg tcgcttggtt atttccaaaa atcaaattaa
     3181 ttttattaaa ctattagaac gagcatgttt tgtctatatg ctacagaaga taaaaaataa
     3241 taggagttaa cagttataaa acaacacact ttgtttctat tgattgttga ccacactggg
     3301 gtctcattaa gttagattaa agacacacta actgggtcaa aagcagcaga ttgatttcat
     3361 agcaccaggg taaactttct aacactttta cggcaatcat atacattaaa attaaataca
     3421 gaccacgact gaacaaggag gatgatctcc aatattaaac aaagagactt gtgcctattt
     3481 ctctgagggt aaacatgacc tctcaagtta gcaagttggt tttaacacta caaaaatagt
     3541 taagactgca atcccagaat aaagtattgg ttttaaccaa tcaatatagt acagtaaaca
     3601 tccatttgtt ttgttgaaac gttaaacaaa tctgaccaaa gctattagct tatataaaac
     3661 aggtttgcct tctatgtagc tgaaaacacc acaggcccga ttttgctact gtgtaaaaca
     3721 tttcagcaag atttttttat tgcatttttt tttactgaat cgttcaaaca ttttatcatt
     3781 ttagtttgtt cattcattgc aactggaaaa acaacacatc acacaaccgc acatatttca
     3841 gcaataagta caataaaaca ctcaaataaa aaaaacattt taaatctctt tgtatttttg
     3901 accgctgttt cgcgtaattt cacggtaaaa ctctggaaat ctccactaca ttcctctcag
     3961 cggctcctct caatgacagc tgaagaagtg acgcggctgc ctgctgtgtt ttgattggtc
     4021 gaattcactg gaggcttcca gaacagtgta gagtctgaac gggtgcgcgc tctgctgtat
     4081 ttaaagggcg aaagagagac cgcagagaaa ctcaaccgaa gagaagcgac ttgacaaaga
     4141 agaaaagagc agcctgacag gactttttcc ccgacgaggt gtttattcgc tctatttaag
     4201 aatctactgt aaggtaagtc tcaatatatt gtactctatt ggctaatcag aattatatag
     4261 agattatatg tacttaatgt caaaaaatca actttgtata tgtaatcttt ttacatgtgg
     4321 actgcctatg ttcatcttat tttaggtcta ctagaaaatt atatttcccg ttttcacaat
     4381 aaggattttt aaaaaaagca atgaacagac gggcatttac tttatgttgc tgacattatt
     4441 ttatatgagc ataataacca taaatactag caaatgtcct aaatgaattt gtgttaatgt
     4501 tgtctacaaa agaaaattag cgttttactt gtacaactaa taataacttg gttattaaga
     4561 gaatttcact tgttgactag aaaaatcctt tcataatgaa acaattgcac cataaattgt
     4621 ataaatataa aattaattct aattgttttt ttttcctgcc aagtttgtac aaaaaagcag
     4681 gctatggtga gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
     4741 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
     4801 cagaccatga gaatcaaggc ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
     4861 gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc acacccaggg catccccgac
     4921 ttctttaagc agtccttccc cgagggcttc acatgggaga gagtcaccac atacgaagac
     4981 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
     5041 gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc
     5101 ggctgggagg cctccaccga gaccctgtac cccgctgacg gcggcctgga aggcagagcc
     5161 gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttgaa gaccacatac
     5221 agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt ggacagaaga
     5281 ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga ggtggctgtg
     5341 gccagatact gcgacctccc tagcaaactg gggcacagaa acccagcttt cttgtacaaa
     5401 gtggctatgc ccagcaggac ggaccccaag atggaccgga gcggcggccg cgtccgtctg
     5461 aaggcgcact acggcgggga catcctgatt accagcgtgg atgccatgac aacattcaag
     5521 gacctctgtg aggaagtgcg agacatgtgt ggcctgcacc agcagcaccc actcaccctc
     5581 aagtgggtgg acagtgaagg tgacccttgt actgtgtcct cacagatgga gctggaggcc
     5641 ttccgcctgg cctgtcaggg cagggacgaa gtgctcatca tccacgtttt cccaagcatc
     5701 ccagaacaac cgggcatgcc ttgtcctgga gaagacaagt ccatctaccg ccgtggagcc
     5761 agaagatgga ggaagctata ccgagccaac ggccacctct tccaagccaa gcgctttaac
     5821 aggagagcgt actgtggcca gtgcagcgaa aggatatggg gcctcgcgag gcaggggtac
     5881 aggtgcatca actgcaagct gcttgtccat aaacgctgcc acgtcctcgt cccgctgacc
     5941 tgcaggaggc atatggattc tgtcatgcct tcccaagagc ctccagtaga tgacaagaac
     6001 gatggtgtag accttccttc agaagaaact gatggaattg cttatatttc ttcatctcgg
     6061 aaacatgaca atatcaaaga tgattctgag gaccttaagc ctgtcatcga tggggtggat
     6121 gggatcaaaa tctctcaggg gctggggctg caagactttg acctcatcag agtcatcggg
     6181 cgtggaagct atgccaaggt cctcctggtg cggttgaaga aaaacgacca gatttacgcc
     6241 atgaaggtgg tgaagaagga gctcgtccac gacgatgagg atatcgactg ggtgcagaca
     6301 gagaagcacg tgttcgagca ggcatccagc aaccccttcc tggttggctt acactcctgc
     6361 ttccagacaa cgagccggtt gttcctggtc atcgagtatg tcaacggggg ggacctcatg
     6421 ttccacatgc agaggcagag gaagcttcca gaggaacacg ccaggttcta tgctgctgag
     6481 atctgtatcg ctctcaactt cctacatgag agagggatca tctaccggga cctaaaactg
     6541 gacaacgtcc tcctcgatgc cgatggacac attaagctga cggactacgg catgtgcaag
     6601 gaaggcctag gccccggcga cacaacaagc actttttgtg gaaccccgaa ctatatcgcc
     6661 cccgaaatcc tgcgaggaga agagtacggg ttcagcgtgg actggtgggc gctgggtgtc
     6721 cttatgtttg agatgatggc tgggcgctcc ccctttgaca tcatcacaga caaccctgac
     6781 atgaatactg aagactacct tttccaagtt atcctggaaa agccaattcg gattccccgt
     6841 ttcctgtctg tcaaggcctc acacgtcttg aaaggatttt taaataagga tcccaaagag
     6901 aggcttggct gccggccgca gactgggttt tccgacatca agtcccatgc cttcttccga
     6961 agcatagact gggacctgct tgaaaagaag cagaccctgc ctcccttcca gccccagatc
     7021 acagatgact atggcctgga caacttcgac acgcagttca ccagcgagcc cgtacagctg
     7081 accccagatg atgaggacgt cataaagagg atcgaccagt ccgagttcga aggcttcgag
     7141 tacatcaacc cgcttctgct gtctgctgag gagtccgtgc tcgagaagct gaaccctcct
     7201 gatgagagtg gccccggctg catgagctgc aagtgtgtgc tctcctaagc aactttatta
     7261 tacatagttg ataattcact ggccgtcgtt ttacggtacc atcgatgatg atccagacat
     7321 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     7381 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     7441 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     7501 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatca
     7561 gatctgcgaa gatacggcca cgggtgctct tgatcctgtg gctgattttg gactgtgctg
     7621 ctcgcagctg ctgatgaatc acatacttcc tccattttct tccactgatt gactgttata
     7681 atttccctaa tttccaggtc aaggtgctgt gcattgtggt aatagatgtg acatgacgtc
     7741 acttccaaag gaccaatgaa catgtctgac caatttcata taatgtgaaa acgattttca
     7801 taggcagaat aaataacatt taaattaaac tgggcatcag cgcaattcaa ttggtttggt
     7861 aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa
     7921 taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta
     7981 aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag
     8041 tacagtaatc aagtaaaatt actcaagtac tttacacctc tggttcttga ccccctacct
     8101 tcagcaagcc cagcagatcc actagttcta gagcggccgc caccgcggtg gagctccagc
     8161 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     8221 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8281 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8341 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8401 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8461 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8521 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8581 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8881 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8941 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9001 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9061 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9181 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9241 aacgaaaact cacgttaagg cattttggtc atgagattat caaaaaggat cttcacctag
     9301 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9361 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9421 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9481 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9541 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9601 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9661 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9721 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9781 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9841 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9901 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9961 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10021 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10081 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10141 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10201 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10261 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10321 ataggggttc cgcgcacatt tccccgaaaa gtg
//
