LOCUS       sequence-203598-        9179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock inducible transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C
  TITLE     Interkinetic nuclear migration is centrosome independent and ensures
            apical cell division to maintain tissue integrity.
  JOURNAL   Dev Cell. 2015 Jan 26;32(2):203-19. doi: 
            10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15.
  PUBMED    25600237
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.12.001"; journalName: "Dev Cell"; date: 
            "2015-01-26- 26"; volume: "32"; issue: "2"; pages: "203-19"
COMMENT     Sequence Label: HS-Utrophin-GFP
FEATURES             Location/Qualifiers
     source          1..9179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2864..2888
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2891..2893,2894..2896,2897..3607)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2891..2893,2894..2896,2897..3607)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2936..2957)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3197..3216)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3544..3565
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4404..4428)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4433..4567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4457..4476)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4511..4530
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6709..6896,6897..6908)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6942..6958)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6942..6958)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6995..7015)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6995..7013)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7034..7050)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7034..7050)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7047..7069)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7058..7074
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7082..7088,7089..7106,7107..7112))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7127..7148
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7265..7282)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7436..8024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7516..7535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8195..8986,8987..9055))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8818..8837
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9056..9160)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttgcttcag gggtgtcgct tggttatttc caaaaatcaa
     1381 attaatttta ttaaactatt agaacgagca tgttttgtct atatgctaca gaagataaaa
     1441 aataatagga gttaacagtt ataaaacaac acactttgtt tctattgatt gttgaccaca
     1501 ctggggtctc attaagttag attaaagaca cactaactgg gtcaaaagca gcagattgat
     1561 ttcatagcac cagggtaaac tttctaacac ttttacggca atcatataca ttaaaattaa
     1621 atacagacca cgactgaaca aggaggatga tctccaatat taaacaaaga gacttgtgcc
     1681 tatttctctg agggtaaaca tgacctctca agttagcaag ttggttttaa cactacaaaa
     1741 atagttaaga ctgcaatccc agaataaagt attggtttta accaatcaat atagtacagt
     1801 aaacatccat ttgttttgtt gaaacgttaa acaaatctga ccaaagctat tagcttatat
     1861 aaaacaggtt tgccttctat gtagctgaaa acaccacagg cccgattttg ctactgtgta
     1921 aaacatttca gcaagatttt tttattgcat ttttttttac tgaatcgttc aaacatttta
     1981 tcattttagt ttgttcattc attgcaactg gaaaaacaac acatcacaca accgcacata
     2041 tttcagcaat aagtacaata aaacactcaa ataaaaaaaa cattttaaat ctctttgtat
     2101 ttttgaccgc tgtttcgcgt aatttcacgg taaaactctg gaaatctcca ctacattcct
     2161 ctcagcggct cctctcaatg acagctgaag aagtgacgcg gctgcctgct gtgttttgat
     2221 tggtcgaatt cactggaggc ttccagaaca gtgtagagtc tgaacgggtg cgcgctctgc
     2281 tgtatttaaa gggcgaaaga gagaccgcag agaaactcaa ccgaagagaa gcgacttgac
     2341 aaagaagaaa agagcagcct gacaggactt tttccccgac gaggtgttta ttcgctctat
     2401 ttaagaatct actgtaaggt aagtctcaat atattgtact ctattggcta atcagaatta
     2461 tatagagatt atatgtactt aatgtcaaaa aatcaacttt gtatatgtaa tctttttaca
     2521 tgtggactgc ctatgttcat cttattttag gtctactaga aaattatatt tcccgttttc
     2581 acaataagga tttttaaaaa aagcaatgaa cagacgggca tttactttat gttgctgaca
     2641 ttattttata tgagcataat aaccataaat actagcaaat gtcctaaatg aatttgtgtt
     2701 aatgttgtct acaaaagaaa attagcgttt tacttgtaca actaataata acttggttat
     2761 taagagaatt tcacttgttg actagaaaaa tcctttcata atgaaacaat tgcaccataa
     2821 attgtataaa tataaaatta attctaattg tttttttttc ctgccaagtt tgtacaaaaa
     2881 agcaggctgg atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2941 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     3001 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3061 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     3121 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3181 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3241 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3301 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3361 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3421 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3481 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     3541 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     3601 gtacaagtcc ggaaccatgg ccaagtatgg agaacatgaa gccagtcctg acaatgggca
     3661 gaacgaattc agtgatatca ttaagtccag atctgatgaa cacaatgacg tacagaagaa
     3721 aacctttacc aaatggataa atgctcgatt ttcaaagagt gggaaaccac ccatcaatga
     3781 tatgttcaca gacctcaaag atggaaggaa gctattggat cttctagaag gcctcacagg
     3841 aacatcactg ccaaaggaac gtggttccac aagggtacat gccttaaata acgtcaacag
     3901 agtgctgcag gttttacatc agaacaatgt ggaattagtg aatatagggg gaaccgacat
     3961 tgtggatgga aatcacaaac tgactttggg gttactttgg agcatcattt tgcactggca
     4021 ggtgaaagat gtcatgaagg atgtcatgtc ggacctgcag cagacgaaca gtgagaagat
     4081 cctgctcagc tgggtgcgtc agaccaccag gccctacagc caagtcaacg tcctcaactt
     4141 caccaccagc tggacagatg gactcgcctt taatgctgtc ctccaccgac ataaacctga
     4201 tctcttcagc tgggataaag ttgtcaaaat gtcaccaatt gagagacttg aacatgcctt
     4261 cagcaaggct caaacttatt tgggaattga aaagctgtta gatcctgaag atgttgccgt
     4321 tcggcttcct gacaagaaat ccataattat gtatttaaca tctttgtttg aggtgctacc
     4381 tcagcaagtc accatagact aagacccagc tttcttgtac aaagttgatg tagatccaga
     4441 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4501 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4561 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     4621 ggttttttaa ttcgcggccg cggcgccaat gcattgggcc ctcgagcata tgagatctgt
     4681 cccgatcttt ctcttctgtg ctgtcaattt agagtagttt ttgaggtaat ttgggtgcat
     4741 tctctgtaaa aacaacaaat aaatatttat attgtatgca ataaatgtaa caattacaca
     4801 tacactctct gattacagca ttaaagggtt agttcaccca aaaatgaaaa gatgtcatta
     4861 atgactcacc ctcatgtcgt tccaagcccg taacacctcc gttcatcttc ggaacacagt
     4921 ttaagatatt ttagatttag tccgagagct ttctgtgcct ccattgagaa tgtatgtacg
     4981 gtatactgtc catgtccaga aaggtaataa aaacatcaaa gtagtccatg tgacatcagt
     5041 gggttaggta gaattatttg aagcatcgaa aatacatttt ggtccaaaaa taacaaaacc
     5101 tacgacttta ttcagcattg tagcgtcact gcgaagtcgt gaacgcggat tgacaacaga
     5161 cccggaagag aatacaatgc cgaataaagt cgtaggtttt gttatttttg gaccaaaatg
     5221 tattcgatgc ttcaaaaaat tctaactaac ccactgatgt cacatggact actttgatgt
     5281 ttttattacc tttctggaca tggacagtat accgtacata cattctcaat ggaggcacag
     5341 aaagctctcg gactaaatct aaaatatctt aaactgtgtt ctgaagatga acggaggtct
     5401 tacgggcttg gaacgacatg agggcgagtc attaatgaca ttattttcat ttttgggtga
     5461 actaaccctt taataaaact gacatttgca gccaattgca cactttataa tgttaaatac
     5521 ttgttgattt tagaaataca gtagcattta gtgacaatca aacattaatt ttatttctga
     5581 ttatattgac aaaaacacag gactgtaagg ctgacatgac agggtgaatt gatttcatta
     5641 cattagtttt gatggtaaca cttttacaat aaggttcgtt agttaactac attagttaac
     5701 atgaactaat aatgaactgc acgtatacat gttaatttca acatttacta atactttata
     5761 tcttgttaac attagttaat gcactgtgaa ctaacatgaa caaacaatga acatctgtat
     5821 ttctattaac taacgttaac aaagattaac agatacagta acaaatgtat tgctcatggt
     5881 tatttaatgt tagttaatac atgaactaat caacctcatt gtaaagtgtt accgttttga
     5941 caatacaaca tgtttggaaa aaaatttctc gcttggtttt cttgtgtttt gcatgaattc
     6001 aaacacaatg agagcacgga atgataatga ttattcaaag ctgcaatagg cgccaaattt
     6061 cccgtggacc taacgttacc aattacaaat gcattttagc attttattac gtgagcctta
     6121 tattgtcaat cgcatgcatt agtgaaaata ggcctactac acatataaaa ggcgcgccat
     6181 caacgtcaaa acatgcacac ccaaaaaaaa aaaaaaaaaa aaaaaaaaaa anctatcagt
     6241 aaaacaaaat acttaatgta cttacctcaa tatgcttcct taggtttgat ggcgaacttt
     6301 tgaaggccga tatttcttta ttaagcggga gacagaggac acatttcatc ttgaatgaat
     6361 ctttatttac accacttaaa gaaaagaatt cgcgaagata cggccacggg tgctcttgat
     6421 cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat acttcctcca
     6481 ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg tgctgtgcat
     6541 tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg tctgaccaat
     6601 ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa ttaaactggg
     6661 catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa gtgatctcca
     6721 aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta cttttttttc
     6781 taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact caagtaaagt
     6841 aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc aagtacttta
     6901 cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta gttctagagc
     6961 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct
     7021 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7081 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7141 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7201 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7261 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7321 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7381 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7441 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7501 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7561 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7621 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7681 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7741 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7801 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7861 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7921 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7981 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8041 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8101 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8161 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8221 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8281 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8341 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8401 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8461 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8521 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8581 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8641 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8701 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8761 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8821 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8881 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8941 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9001 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9061 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9121 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtg
//
