LOCUS       sequence-203600-        9119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock inducible transgenesis, PACT is a centriol marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C
  TITLE     Interkinetic nuclear migration is centrosome independent and ensures
            apical cell division to maintain tissue integrity.
  JOURNAL   Dev Cell. 2015 Jan 26;32(2):203-19. doi: 
            10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15.
  PUBMED    25600237
REFERENCE   2  (bases 1 to 9119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.12.001"; journalName: "Dev Cell"; date: 
            "2015-01-26- 26"; volume: "32"; issue: "2"; pages: "203-19"
COMMENT     Sequence Label: HS-GFP-PACT
FEATURES             Location/Qualifiers
     source          1..9119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2536..2547,2548..2710)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     3083..3099
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3119..3139
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4659..4683
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4686..4695
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4692..4694,4695..4697,4698..5408)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4692..4694,4695..4697,4698..5408)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4737..4758)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4998..5017)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5345..5366
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5409..5474
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(5488..5512)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6016..6036)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6044..6060)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6076..6210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6100..6119)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6154..6173
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6649..6836,6837..6848)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6882..6898)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6882..6898)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6935..6955)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6935..6953)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6974..6990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6987..7009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6998..7014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7022..7028,7029..7046,7047..7052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7067..7088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7205..7222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7376..7964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7456..7475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8135..8926,8927..8995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8758..8777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8996..9100)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgttgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggttgg ttggtagatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattgattga ttgattgatt gattgattga ttgattgatt ggtagtcaaa ataagaaata
     1561 atttccacag attcattaca gaaatgatta aatgcataca taaaaaactn gggggggggg
     1621 gggatacaac aacacactta agtcacattt gcctacgtaa agaaaagtaa agaaaatcaa
     1681 tagctatatt ttacatctcc tttttttgct gtcttttaat tagccttgtt ttgctgttat
     1741 cttgattgaa actgtaactt cttcacctgc ttcttttctt tgtaggtttt gccagcccag
     1801 aaacaggcat ggctgtgcaa ggccagagca ccatgcacaa tgcgctgcat gtcttcatga
     1861 acggctcaat gtcctcagtc cagggctcag ccaacgaccc catcttcctc cttcaccatg
     1921 ctttcattga caggtaacaa acacgtcatg acattagact gcacagtttt tgacaaagtt
     1981 catacaatct gttgtttata gctgctacaa ttagtgaagt ttgtgaatgt acttggatga
     2041 gcagcgaaag atcaattgag atcaattgtt agagtttggt tgccctgcag agcaaagaac
     2101 aaaaaataat ctggtggctt tactgcgtga ggttattatt ggtggaatag aaacacaaaa
     2161 cataattgca tttatttgtt taatttttta tcttatctta actttcatct tgcatatttg
     2221 tttcttacat catttctagc atctttgagc gctggctaag aactcatcag cctccccggt
     2281 ccatctaccc acgtaccaat gcaccaattg gccacaatga cggctactac atggtgccat
     2341 tccttcctct ttataggaat ggagactacc tcctgtccaa caatgctctt ggatacgagt
     2401 acgcctacct gttggaccca ggtcattgca caacaccaga aatgccctct gatctgcaaa
     2461 agacgtgaat atctgttcag acacccatat ccactctgtt ccacacaggt cagaggtttg
     2521 tccaggagtt cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac
     2581 aagtacttag ggaaaatttt actcaattaa aagtaaaagt atctggctag aatcttactt
     2641 gagtaaaagt aaaaaagtac tccattaaaa ttgtacttga gtattaagga agtaaaagta
     2701 aaagcaagaa agaaaactag agattcttgt ttaagctttt aatctcaaaa aacattaaat
     2761 gaaatgcata caaggtttta tcctgcttta gaactgtttg tatttaatta tcaaactata
     2821 agacagacaa tctaatgcca gtacacgcta ctcaaagttg taaaacctca gatttaactt
     2881 cagtagaagc tgattctcaa aattgttagt gtcaagccta gctcttttgg ggctgaaaag
     2941 caatcctgca gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag gtgtattagt
     3001 cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac
     3061 acaggccaga tgggccctcg agcaggaaac agctatgacc atgattacgc caagctatca
     3121 actttgtata gaaaagttgc ttcaggggtg tcgcttggtt atttccaaaa atcaaattaa
     3181 ttttattaaa ctattagaac gagcatgttt tgtctatatg ctacagaaga taaaaaataa
     3241 taggagttaa cagttataaa acaacacact ttgtttctat tgattgttga ccacactggg
     3301 gtctcattaa gttagattaa agacacacta actgggtcaa aagcagcaga ttgatttcat
     3361 agcaccaggg taaactttct aacactttta cggcaatcat atacattaaa attaaataca
     3421 gaccacgact gaacaaggag gatgatctcc aatattaaac aaagagactt gtgcctattt
     3481 ctctgagggt aaacatgacc tctcaagtta gcaagttggt tttaacacta caaaaatagt
     3541 taagactgca atcccagaat aaagtattgg ttttaaccaa tcaatatagt acagtaaaca
     3601 tccatttgtt ttgttgaaac gttaaacaaa tctgaccaaa gctattagct tatataaaac
     3661 aggtttgcct tctatgtagc tgaaaacacc acaggcccga ttttgctact gtgtaaaaca
     3721 tttcagcaag atttttttat tgcatttttt tttactgaat cgttcaaaca ttttatcatt
     3781 ttagtttgtt cattcattgc aactggaaaa acaacacatc acacaaccgc acatatttca
     3841 gcaataagta caataaaaca ctcaaataaa aaaaacattt taaatctctt tgtatttttg
     3901 accgctgttt cgcgtaattt cacggtaaaa ctctggaaat ctccactaca ttcctctcag
     3961 cggctcctct caatgacagc tgaagaagtg acgcggctgc ctgctgtgtt ttgattggtc
     4021 gaattcactg gaggcttcca gaacagtgta gagtctgaac gggtgcgcgc tctgctgtat
     4081 ttaaagggcg aaagagagac cgcagagaaa ctcaaccgaa gagaagcgac ttgacaaaga
     4141 agaaaagagc agcctgacag gactttttcc ccgacgaggt gtttattcgc tctatttaag
     4201 aatctactgt aaggtaagtc tcaatatatt gtactctatt ggctaatcag aattatatag
     4261 agattatatg tacttaatgt caaaaaatca actttgtata tgtaatcttt ttacatgtgg
     4321 actgcctatg ttcatcttat tttaggtcta ctagaaaatt atatttcccg ttttcacaat
     4381 aaggattttt aaaaaaagca atgaacagac gggcatttac tttatgttgc tgacattatt
     4441 ttatatgagc ataataacca taaatactag caaatgtcct aaatgaattt gtgttaatgt
     4501 tgtctacaaa agaaaattag cgttttactt gtacaactaa taataacttg gttattaaga
     4561 gaatttcact tgttgactag aaaaatcctt tcataatgaa acaattgcac cataaattgt
     4621 ataaatataa aattaattct aattgttttt ttttcctgcc aagtttgtac aaaaaagcag
     4681 gctgggccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4741 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4801 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     4861 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     4921 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4981 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     5041 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     5101 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     5161 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     5221 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5281 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     5341 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     5401 tgtacaagtc cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
     5461 cgggcccggg atccaccgga tctagacacc cagctttctt gtacaaagtg gctgccaaca
     5521 ttgaagccat cattgcctct gaaaaagaag tatggaacag agaaaaattg actctccaga
     5581 aatctttgaa aagggcagag gctgaagtat acaaactgaa agctgaacta agaaatgact
     5641 ctttacttca aactctgagc cctgattctg aacatgtcac tttaaagaga atttatggta
     5701 aatacttgag ggcagaaagt tttcgaaagg ctctcattta ccagaagaaa tacctgctgc
     5761 tgttactggg tgggttccag gaatgtgaag atgccacctt ggccctgctt gcccggatgg
     5821 gggggcagcc agctttcacg gatctagagg tgatcaccaa tcgcccaaag ggcttcacca
     5881 ggtttcggtc ggccgtcaga gtatccattg caatttccag aatgaaattt ttggttcgac
     5941 ggtggcatcg agtcacaggt tctgtttcca tcaatattaa cagagatggc tttggactga
     6001 atcaaggtgc ataggcaact ttattataca tagttgataa ttcactggcc gtcgttttac
     6061 ggtaccatcg atgatgatcc agacatgata agatacattg atgagtttgg acaaaccaca
     6121 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     6181 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     6241 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     6301 atggctgatt atgatcctct agatcagatc tgcgaagata cggccacggg tgctcttgat
     6361 cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat acttcctcca
     6421 ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg tgctgtgcat
     6481 tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg tctgaccaat
     6541 ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa ttaaactggg
     6601 catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa gtgatctcca
     6661 aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta cttttttttc
     6721 taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact caagtaaagt
     6781 aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc aagtacttta
     6841 cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta gttctagagc
     6901 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct
     6961 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7021 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7081 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7141 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7201 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7261 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7321 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7381 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7441 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7501 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7561 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7621 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7681 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7741 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7801 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7861 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7921 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7981 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaaggcatt ttggtcatga
     8041 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8101 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9001 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9061 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtg
//
