LOCUS       sequence-203614-       10309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Xing S, Wallmeroth N, Berendzen KW, Grefen C
  TITLE     Techniques for the Analysis of Protein-Protein Interactions in Vivo.
  JOURNAL   Plant Physiol. 2016 Jun;171(2):727-58. doi: 10.1104/pp.16.00470. 
            Epub 2016 Apr 25.
  PUBMED    27208310
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.00470"; journalName: "Plant Physiol"; date: 
            "2016-06"; volume: "171"; issue: "2"; pages: "727-58"
COMMENT     Sequence Label: pMetOYC-Dest
FEATURES             Location/Qualifiers
     source          1..10309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..382
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     protein_bind    509..633
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(658..663,664..681,682..688)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             742..1401
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(808..827)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1743..2048
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1927..1946
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2089..2213)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2428..2601
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2602..2775
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             join(2794..3045,3046..3399)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     3337..3358
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             3418..3651
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3803..3822)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3847..3895
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(4197..4216)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4198..4216)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4223..4240)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4223..4239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4232..4254)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4996..6158
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      5321..6158
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(6520..7614)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7615..8020)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(8040..8058)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8126..8230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8231..8299,8300..9091)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8449..8468)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9262..9850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9751..9770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10004..10021
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10138..10159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10174..10179,10180..10197,10198..10204)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10212..10228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10217..10239
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10236..10252
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10236..10252
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        10273..10291
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggatgcaagg gttcgaatcc cttagctctc attatttttt gctttttctc ttgaggtcac
       61 atgatcgcaa aatggcaaat ggcacgtgaa gctgtcgata ttggggaact gtggtggttg
      121 gcaaatgact aattaagtta gtcaaggcgc catcctcatg aaaactgtgt aacataataa
      181 ccgaagtgtc gaaaaggtgg caccttgtcc aattgaacac gctcgatgaa aaaaataaga
      241 tatatataag gttaagtaaa gcgtctgtta gaaaggaagt ttttcctttt tcttgctctc
      301 ttgtcttttc atctactatt tccttcgtgt aatacagggt cgtcagatac atagatacaa
      361 ttctattacc cccatccata cactcgagat gatctctgat gaacagttga actccttggc
      421 catcaccttc ggtattgtga tgatgacttt aattgtcatt taccatgctg ttgactccac
      481 catgtctcct aagaacggtg gatctagaac aagtttgtac aaaaaagctg aacgagaaac
      541 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
      601 atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt
      661 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt
      721 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
      781 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
      841 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
      901 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
      961 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     1021 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     1081 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     1141 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     1201 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     1261 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     1321 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     1381 gagtggcagg gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat
     1441 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     1501 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct
     1561 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     1621 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     1681 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg
     1741 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     1801 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     1861 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     1921 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     1981 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     2041 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     2101 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     2161 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgattggt
     2221 ggtggcggat ccggtggagg tggatcaggt atccctccag atcaacaaag attgatcttt
     2281 gccggtaagc agctagaaga cggtagaacg ctgtctgatt acaacattca gaaggagtcc
     2341 accttacatc ttgtgctaag gctaagaggt ggtatgcaca gatcagcttg cggccgcatg
     2401 gcaggccttg cgcaacacga tgaagccgtg gacaacaaat tcaacaaaga acaacaaaac
     2461 gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc
     2521 caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta
     2581 aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca aaacgcgttc
     2641 tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt catccaaagt
     2701 ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa gctaaatgat
     2761 gctcaggcgc cgaaagtaga cgcgagcggc cgcatgaaag cgttaacggc caggcaacaa
     2821 gaggtgtttg atctcatccg tgatcacatc agccagacag gtatgccgcc gacgcgtgcg
     2881 gaaatcgcgc agcgtttggg gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg
     2941 ctggcacgca aaggcgttat tgaaattgtt tccggcgcat cacgcgggat ccgtctgttg
     3001 caggaagagg aagaagggtt gccgctggta ggtcgtgtgg ctgccggtga accacttctg
     3061 gcgcaacagc atattgaagg tcattatcag gtcgaccctt ccttattcaa gccgaatgct
     3121 gatttcctgc tgcgcgtcag cgggatgtcg atgaaagata tcggcattat ggatggtgac
     3181 ttgctggcag tgcataaaac tcaggatgta cgtaacggtc aggtcgttgt cgcacgtatt
     3241 gatgacgagg ttaccgttaa gcgcctgaaa aaacagggca ataaagtcga actgttgcca
     3301 gaaaatagcg agtttaaacc aattgtcgta gatcttcgtc agcagagctt caccattgaa
     3361 gggctggcgg ttggggttat tcgcaacggc gactggctgg aattcccggg gatctgggcc
     3421 cccccgaccg atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg
     3481 gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg
     3541 ggtccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc
     3601 gagtttgagc agatgtttac cgatgccctt ggaattgacg agtacggtgg gtagggggcg
     3661 cgaccggacc cgcatccccc gtctgggttt tcccctcccg tccccggttc gtatccacaa
     3721 taaacacgag cacatacatt acaaaaacct gcggttgtcg tctgattatt tggtggatcc
     3781 gggggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     3841 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
     3901 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     3961 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     4021 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag
     4081 ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg
     4141 cgtggggtca ggtccacgac ccaagctggc cgccaccgcg gtggagctcc aattcgccct
     4201 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4261 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4321 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     4381 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataatcgctg
     4441 ggccattctc atgaagaata tcttgaattt attgtcatat tactagttgg tgtggaagtc
     4501 ctaatatcgg tgatcaatat agtggttgac atgctggcta gtcaacattg agccttttga
     4561 tcatgcaaat atattacggt attttacaat caaatatcaa acttaactat tgactttata
     4621 acttatttag gtggtaacat tcttataaaa aagaaaaaaa ttactgcaaa acagtactag
     4681 cttttaactt gtatcctagg ttatctatgc tgtctcacca tagagaatat tacctatttc
     4741 agaatgtatg tccatgattc gccgggtaaa tacatataat acacaaatct ggcttaataa
     4801 agtctataat atatctcata aagaagtgct aaattggcta gtgctatata tttttaagaa
     4861 aatttctttt gactaagtcc atatcgactt tgtaaaagtt cacattagca tacatatatt
     4921 acacgagcca gaaatagtaa cttttgccta aatcacaaat tgcaaaattt aattgcttgc
     4981 aaaaggtcac atgcttataa tcaacttttt taaaaattta aaatactttt ttatttttta
     5041 tttttaaaca taaatgaaat aatttattta ttgtttatga ttaccgaaac ataaaacctg
     5101 ctcaagaaaa agaaactgtt ttgtccttgg aaaaaaagca ctacctagga gcggccaaaa
     5161 tgccgaggct ttcatagctt aaactcttta cagaaaatag gcattataga tcagttcgag
     5221 ttttcttatt cttccttccg gttttatcgt cacagtttta cagtaaataa gtatcacctc
     5281 ttagagttcg atgataagct gtcaaacatg agaattaatt ccacatgtta aaatagtgaa
     5341 ggagcatgtt cggcacacag tggaccgaac gtggggtaag tgcactaggg tccggttaaa
     5401 cggatctcgc attgatgagg caacgctaat tatcaacata tagattgtta tctatctgca
     5461 tgaacacgaa atctttactt gacgacttga ggctgatggt gtttatgcaa agaaaccact
     5521 gtgtttaata tgtgtcactg tttgatatta ctgtcagcgt agaagataat agtaaaagcg
     5581 gttaataagt gtatttgaga taagtgtgat aaagttttta cagcgaaaag acgataaata
     5641 caagaaaatg attacgagga tacggagaga ggtatgtaca tgtgtattta tatactaagc
     5701 tgccggcggt tgtttgcaag accgagaaaa ggctagcaag aatcgggtca ttgtagcgta
     5761 tgcgcctgtg aacattctct tcaacaagtt tgattccatt gcggtgaaat ggtaaaagtc
     5821 aaccccctgc gatgtatatt ttcctgtaca atcaatcaaa aagccaaatg atttagcatt
     5881 atctttacat cttgttattt tacagatttt atgtttagat cttttatgct tgcttttcaa
     5941 aaggcttgca ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat
     6001 ttcttagcat ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc
     6061 acacctccgc ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt
     6121 tctggcgtca gtccaccagc taacataaaa tgtaagctct cggggctctc ttgccttcca
     6181 acccagtcag aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca
     6241 aacaagggaa taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct
     6301 tttggaaata cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac
     6361 gactcatctc catgcagttg gacgatcgat gataagctgt caaacatgag aattaattct
     6421 accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat
     6481 aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac
     6541 ttcttcggcg acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt
     6601 tctgatacct gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg
     6661 caagttcaat gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac
     6721 cttattcttt ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt
     6781 gttcttgtct ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat
     6841 aacggaggct tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt
     6901 taggtgggtt gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac
     6961 cttcaatgta ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga
     7021 agaggccaaa acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag
     7081 ggccatgaaa gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact
     7141 atcccaagcg acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac
     7201 taattctctg acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa
     7261 gtctaaaaga gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc
     7321 tttacggatt tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc
     7381 acccacagca cctaacaaaa cggcatcagc cttcttggag gcttccagcg cctcatctgg
     7441 aagtggaaca cctgtagcat cgatagcagc accaccaatt aaatgatttt cgaaatcgaa
     7501 cttgacattg gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat
     7561 ttcttgacca acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat
     7621 ggtatatcct tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa
     7681 agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca
     7741 acttcaagta ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag
     7801 ccggttccgg cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat
     7861 gcgtcaggcg acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg
     7921 atagcgcccc tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc
     7981 gaactcttgc atcttacgat acctgagtat tcccacagtt aattcttgaa gacgaaaggg
     8041 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     8101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8161 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8221 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8281 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8341 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8401 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8461 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8521 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8581 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8641 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8701 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8761 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8821 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8881 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8941 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9001 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9061 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9121 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9181 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9241 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9301 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9361 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9421 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9481 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9541 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9601 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9661 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9721 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9781 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9841 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9901 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9961 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10021 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    10081 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    10141 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    10201 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    10261 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctgggtacc
//
