LOCUS       sequence-203615-        8499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Grefen C, Lalonde S, Obrdlik P
  TITLE     Split-ubiquitin system for identifying protein-protein interactions 
            in membrane and full-length proteins.
  JOURNAL   Curr Protoc Neurosci. 2007 Oct;Chapter 5:Unit 5.27. doi: 
            10.1002/0471142301.ns0527s41.
  PUBMED    18428659
REFERENCE   2  (bases 1 to 8499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Neurosci."; date: "2007-10"; volume: "Chapter 5:Unit 5.27. doi"; 
            pages: " 10.1002/0471142301.ns0527s41"
COMMENT     Sequence Label: pNX32-Dest
FEATURES             Location/Qualifiers
     source          1..8499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    483..607
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        781..883
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             884..1543
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(950..969)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1885..2190
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2069..2088
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2231..2355)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2369..2458
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      2491..2678
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2546..2570)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2705..2721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2705..2721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2718..2740)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2729..2745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2753..2759,2760..2777,2778..2783))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2798..2819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2936..2953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3107..3695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3187..3206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3866..4657,4658..4726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4489..4508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4727..4831)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4858..6200
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(5821..5868)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6241..6259
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6297..6319)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6419..6438
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6459..6740
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6741..7415
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7518..7973)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7655..7676)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7867..7886
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8099..8121
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8113..8130
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8114..8130
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(8153..8499,1..358)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 tgttgtctca ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt
       61 aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa
      121 gcacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat
      181 acggccttcc ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca
      241 agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt
      301 tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactcg
      361 agatgcagat tttcgtcaag actttgaccg gtaaaaccgg aacattggaa gttgaatctt
      421 ccgataccat cgacaacgtt aagtcgaaaa ttcaagacaa ggaaggaatc cctggtgggt
      481 cgacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
      541 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
      601 cactatggcg gccgcgggtg atgctgccaa cttagcggcc gctaagttgg cagcatcacc
      661 cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa
      721 tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt
      781 tgatcggcac gcaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      841 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
      901 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
      961 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1021 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1081 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1141 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1201 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1261 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1321 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1381 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1441 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     1501 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt
     1561 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     1621 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     1681 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     1741 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     1801 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     1861 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     1921 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     1981 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2041 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2101 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2161 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2221 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2281 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2341 cttgtacaaa gtggttggtg gtggcggata cccatacgat gttcctgact atgcgggcta
     2401 tccctatgac gtcccggact atgcaggatc ctatccatat gacgttccag attacgctgc
     2461 tcagtgctga ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt
     2521 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     2581 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     2641 tagtatgagg tcgctcttat tgaccacacc tctaccggca gcatgcaagc ttggcgtaat
     2701 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2761 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2821 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2881 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2941 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3001 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3061 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3121 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3181 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3241 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3301 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3361 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3421 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3481 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3541 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3601 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3661 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3721 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3781 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3841 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3901 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3961 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4021 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4081 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4141 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4201 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4261 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4321 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4381 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4441 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4501 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4561 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4621 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4681 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4741 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4801 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac
     4861 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
     4921 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
     4981 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt
     5041 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
     5101 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
     5161 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
     5221 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
     5281 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
     5341 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
     5401 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
     5461 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
     5521 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
     5581 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
     5641 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
     5701 ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg
     5761 tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct
     5821 gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg
     5881 aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc
     5941 gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt
     6001 gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt
     6061 acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc
     6121 tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc
     6181 atttcctttg atattggatc atactaagaa accattatta tcatgacatt aacctataaa
     6241 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6301 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     6361 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     6421 gcatcagagc agattgtact gagagtgcac catagatcaa cgacattact atatatataa
     6481 tataggaagc atttaataga acagcatcgt aatatatgtg tactttgcag ttatgacgcc
     6541 agatggcagt agtggaagat attctttatt gaaaaatagc ttgtcacctt acgtacaatc
     6601 ttgatccgga gcttttcttt ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag
     6661 gagagggcca agagggaggg cattggtgac tattgagcac gtgagtatac gtgattaagc
     6721 acacaaaggc agcttggagt atgtctgtta ttaatttcac aggtagttct ggtccattgg
     6781 tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctagat tccgatgctg
     6841 acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg gttattgcaa
     6901 ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg aaatacttgg
     6961 ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat gattacggca
     7021 ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag ttcctcggtt
     7081 tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc agtgcagctt
     7141 cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg acaggtgaac
     7201 ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc gaaagcttac
     7261 attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt agattaaatg
     7321 gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa gactctaaca
     7381 aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag tatttattta
     7441 agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat accgcacaga tgcgtaagga
     7501 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     7561 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     7621 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     7681 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     7741 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     7801 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     7861 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     7921 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
     7981 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     8041 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     8101 cagtcacgac gttgtaaaac gacggccagt gccaagcttt cgcgagctcg atatcctttt
     8161 gttgtttccg ggtgtacaat atggacttcc tcttttctgg caaccaaacc catacatcgg
     8221 gattcctata ataccttcgt tggtctccct aacatgtagg tggcggaggg gagatataca
     8281 atagaacaga taccagacaa gacataatgg gctaaacaag actacaccaa ttacactgcc
     8341 tcattgatgg tggtacataa cgaactaata ctgtagccct agacttgata gccatcatca
     8401 tatcgaagtt tcactaccct ttttccattt gccatctatt gaagtaataa taggcgcatg
     8461 caacttcttt tctttttttt tcttttctct ctcccccgt
//
