LOCUS       sequence-203618-       11034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline regulated expression of Tbx20 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11034)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 11034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_Tbx20
FEATURES             Location/Qualifiers
     source          1..11034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    105..123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    142..160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    179..197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    253..271
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    290..308
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     330..354
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    427..451
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1821..1845)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1915..2376
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1971..1988)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             2381..3091
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2526..2544)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2794..2813
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3124..3712
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3177..3197)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3595..3606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3715..3731)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             4240..4420
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4446..4463)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4452..4676
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4722..5150
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4809..4828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5019..5040
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5159..5179)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5164..5493
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5344..5479
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5406..5425
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5541..5588
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5607..5981
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6111..6232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6148..6167)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6202..6221
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6281..6297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6281..6297)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6294..6316)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6305..6321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6329..6335,6336..6353,6354..6359))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6374..6395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6512..6529)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6683..7271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6763..6782)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7442..8233,8234..8302))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8065..8084
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8303..8407)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8482..8501)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8673..9052
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9054..9252
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9207..9227
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9270..9450
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9497..9622
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10119..10352
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10537..10581
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10879..10996
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acgcgtcccc gaaaagtgcc acctgacgtc tccctatcag tgatagagaa gtcgacacgt
       61 ctcgagctcc ctatcagtga tagagaaggt acgtctagaa cgtctcccta tcagtgatag
      121 agaagtcgac acgtctcgag ctccctatca gtgatagaga aggtacgtct agaacgtctc
      181 cctatcagtg atagagaagt cgacacgtct cgagctccct atcagtgata gagaaggtac
      241 gtctagaacg tctccctatc agtgatagag aagtcgacac gtctcgagct ccctatcagt
      301 gatagagaag gtacccccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      361 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctgcta
      421 gcatcaacaa gtttgtacaa aaaagcaggc tccgaattcg cccttatgga gttcacggcg
      481 tcgcccaagc cccagctctc ctctcgagcc aatgccttct ccatcgccgc gcttatgtcc
      541 agcggcggcc ccaaggagaa ggaggcagca gagaacacca tcaaacccct ggaacaattt
      601 gtggagaaat catcatgtgc ccagccactg ggtgagctga cgagtctgga tgctcacgcg
      661 gagtttggcg gcgggggcgg cagcccatcc tcatcctctc tgtgcacaga gccactgata
      721 cccaccaccc ccatcatccc cagcgaagag atggctaaaa tcgcctgcag cctggaaacg
      781 aaggagctct gggacaaatt ccatgaactg ggcacggaga tgataatcac caagtctggc
      841 aggaggatgt tccccaccat ccgcgtgtca ttttctggag tggatcctga gtccaagtat
      901 atagtcctga tggacatcgt cccggtggac aacaagagat accgctatgc ctaccaccgg
      961 tcatcctggc tggtggctgg caaagctgat cccccgctgc cagccaggct ctacgtgcac
     1021 ccagactccc cctttactgg cgagcagctc ctcaaacaga tggtgtcttt tgaaaaggtg
     1081 aagctcacca acaatgaact ggatcaacac ggccatataa ttttgaattc aatgcataag
     1141 taccagccac gggtgcacat cataaagaag aaagaccaca cggcctcctt gctcaatctg
     1201 aagtcagaag aattcaggac gttcatcttt ccagagacag ttttcacagc agtcacagcc
     1261 taccagaacc aactgataac caagctgaaa atagacagca atccgtttgc caaaggattc
     1321 cgggactcct ccaggctcac tgacattgag agggagagtg tggagagcct gatccagaag
     1381 cattcctatg cccggtcacc catccgcacc tatggggaag aggatgttct gggggaggag
     1441 agtcaaacaa ctcagagtcg aggatcagcc tttacaacat ctgacaattt gtctctcagt
     1501 tcctgggtat cgtcatcttc cagttttcct ggatttcagc atccacagcc cctgactgct
     1561 cttggtacca gtacagcatc catagcgaca ccgattcctc accctataca gggttctctg
     1621 ccaccatata gccgcctggg aatgcctctg accccatctg caatagccag ctccatgcag
     1681 ggaagtggtc ccacgtctcc ttcattccac atgcctagat accatcacta cttccagcag
     1741 gggccctacg ctgccatcca aggacttcgc cactcctccg ctgtgatgac gccatttgta
     1801 tgattccaag ggcgaattcg acccagcttt cttgtacaaa gtggttgatg cggccgcttc
     1861 gagcagacat gagtcgacgt tattttccac catattgccg tcttttggca atgtgagggc
     1921 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     1981 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     2041 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     2101 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     2161 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctcacctcaa gcgtattcaa
     2221 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     2281 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac
     2341 ggggacgtgg ttttcctttg aaaaacacga tgataatacc atggtgagca agggcgagga
     2401 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     2461 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2521 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     2581 ccctcagttc atgtacggct ccaaggccta cgggaagcac cccgccgaca tccccgacta
     2641 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2701 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     2761 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     2821 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     2881 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     2941 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     3001 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     3061 caccggcggc atggacgagc tgtacaagta agtcgaccat aattcgatat caagcttatc
     3121 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3181 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3241 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3301 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3361 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3421 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3481 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3541 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3601 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3661 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     3721 cgtcgacctc gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     3781 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     3841 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3901 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     3961 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     4021 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     4081 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     4141 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     4201 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     4261 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     4321 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     4381 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     4441 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4501 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4561 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4621 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4681 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4741 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4801 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4861 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4921 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4981 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5041 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5101 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     5161 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5221 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5281 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5341 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5401 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5461 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5521 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     5581 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     5641 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     5701 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     5761 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     5821 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     5881 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     5941 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     6001 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6061 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     6121 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6181 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6241 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6301 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6361 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6421 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6481 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6601 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6661 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6841 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7081 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7201 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7441 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7501 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7561 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7621 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7681 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7741 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7801 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7861 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7921 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7981 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8041 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8101 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8161 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8221 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8281 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8341 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8401 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     8461 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     8521 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     8581 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     8641 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     8701 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     8761 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     8821 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     8881 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     8941 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     9001 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     9061 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     9121 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     9181 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     9241 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     9301 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9361 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9421 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     9481 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     9541 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     9601 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     9661 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     9721 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     9781 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     9841 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     9901 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     9961 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    10021 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    10081 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    10141 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    10201 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    10261 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    10321 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    10381 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    10441 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    10501 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    10561 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    10621 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    10681 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    10741 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    10801 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
    10861 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    10921 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    10981 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggtt
//
