LOCUS       sequence-203619-       11249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline regulated expression of Tbx5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 11249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_Tbx5
FEATURES             Location/Qualifiers
     source          1..11249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..25
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    44..62
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    118..136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    155..173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    279..396
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(694..738)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    923..1156
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1653..1778
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1825..2005
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2023..2221)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2048..2068)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2223..2602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2774..2793
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2868..2972
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2973..3041,3042..3833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3191..3210)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4004..4592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4493..4512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4746..4763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4880..4901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4916..4921,4922..4939,4940..4946)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4954..4970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4959..4981
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4978..4994
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4978..4994
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5043..5164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5054..5073)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5108..5127
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5294..5668)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(5687..5734)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5782..6111)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5796..5931
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5850..5869)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6096..6116
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(6125..6553)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6235..6256)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6447..6466
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    6599..6823
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6812..6829
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             6855..7035
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     7544..7560
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7563..8151
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7669..7680
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8078..8098
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8184..8894)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8462..8481)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8731..8749
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    8899..9360
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     9287..9304
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    9430..9454
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11039..11063)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11136..11160)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    11182..11200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11219..11237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtaccttctc tatcactgat agggagctcg agacgtgtcg acttctctat cactgatagg
       61 gagacgttct agacgtacct tctctatcac tgatagggag ctcgagacgt gtcgacttct
      121 ctatcactga tagggagacg ttctagacgt accttctcta tcactgatag ggagctcgag
      181 acgtgtcgac ttctctatca ctgataggga gacgtcaggt ggcacttttc ggggacgcgt
      241 aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
      301 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
      361 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
      421 ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc tgtctctgtc
      481 tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag
      541 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
      601 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
      661 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
      721 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
      781 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
      841 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
      901 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag
      961 gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc
     1021 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc
     1081 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
     1141 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
     1201 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
     1261 tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc
     1321 tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg
     1381 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat
     1441 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag
     1501 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc
     1561 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
     1621 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
     1681 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
     1741 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
     1801 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
     1861 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     1921 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     1981 agatctggtc taaccagaga gacccagtac aggcaaaacg cgctgcttat atagacctcc
     2041 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
     2101 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
     2161 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
     2221 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     2281 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     2341 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     2401 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     2461 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     2521 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     2581 taactagtca ataatcaatg tcaacgcgta tatctggccc gtacatcgcg aagcagcgca
     2641 aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta
     2701 gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac
     2761 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatag gggatcggga
     2821 gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2881 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2941 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3001 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3061 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3121 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3181 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3241 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3301 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3361 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3421 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3481 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3541 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3601 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3661 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3721 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3781 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3841 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3901 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3961 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4021 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4081 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4141 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4201 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4261 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4321 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4381 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4441 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4501 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4561 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4621 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4681 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4741 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4801 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4861 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     4921 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     4981 gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgacggt atacagacat
     5041 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     5101 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     5161 agttggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
     5221 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat
     5281 ctcgtagcac gtgtcagtcc tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt
     5341 cgcgcagggc gaactcccgc ccccacggct gctcgccgat ctcggtcatg gccggcccgg
     5401 aggcgtcccg gaagttcgtg gacacgacct ccgaccactc ggcgtacagc tcgtccaggc
     5461 cgcgcaccca cacccaggcc agggtgttgt ccggcaccac ctggtcctgg accgcgctga
     5521 tgaacagggt cacgtcgtcc cggaccacac cggcgaagtc gtcctccacg aagtcccggg
     5581 agaacccgag ccggtcggtc cagaactcga ccgctccggc gacgtcgcgc gcggtgagca
     5641 ccggaacggc actggtcaac ttggccatgg tttagttcct caccttgtcg tattatacta
     5701 tgccgatata ctatgccgat gattaattgt caacacgtgc tgatcagatc cgaaaatgga
     5761 tatacaagct cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     5821 cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag
     5881 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     5941 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     6001 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     6061 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     6121 aattaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     6181 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     6241 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     6301 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     6361 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     6421 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     6481 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     6541 ctacagggcg cgtggggata ccccctagag ccccagctgg ttctttccgc ctcagaagcc
     6601 atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg
     6661 tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt
     6721 tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg
     6781 ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcgg
     6841 gtttaaacgg gccctgctag agattttcca cactgactaa aagggtctga gggatctcta
     6901 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
     6961 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
     7021 taaccagaga gacccagtac agtccggatg cagctctcgg gccatgtgat gaaatgctag
     7081 gcggctgtca aacctccact ctaatacttc tctctccggg tcatccatcc catgcaggct
     7141 cacagggtgt aacaagcggg tgttctctcc ttcattggct tcttctacct tctcttgctc
     7201 aactggtact agcttgtagc accatccaaa ggtcagtgga tatctgatcc ctggccctgg
     7261 tgtgtagttc tgccaatcag ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat
     7321 atcttgtctt cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg
     7381 gctaagatct acagctgcct tgtaagtcat tggtcttaaa ggtacctgag gtgtgactgg
     7441 aaaacccacc tcctcctcct cttgtgcttc tagccaggca caatcagcat tggtagctgc
     7501 tgtattgcta cttgtgattg ctccatgttt ttctaggtct cgacgaggtc gacggtatcg
     7561 atgcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
     7621 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
     7681 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
     7741 aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
     7801 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
     7861 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
     7921 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
     7981 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
     8041 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
     8101 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tatcgataag
     8161 cttgatatcg aattatggtc gacttacttg tacagctcgt ccatgccgcc ggtggagtgg
     8221 cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
     8281 tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag
     8341 gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc
     8401 tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg
     8461 gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg
     8521 tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc
     8581 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg
     8641 gggatgtcgg cggggtgctt cccgtaggcc ttggagccgt acatgaactg aggggacagg
     8701 atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg
     8761 ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag
     8821 ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg
     8881 cccttgctca ccatggtatt atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt
     8941 cggggggcct agacgttttt taacctcgac taaacacatg taaagcatgt gcaccgaggc
     9001 cccagatcag atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat
     9061 acgcttgagg tgagccattt gactctttcc acaactatcc aactcacaac gtggcactgg
     9121 ggttgtgccg cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg
     9181 tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa
     9241 gaagcttcca gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg
     9301 agaggggaaa gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc
     9361 acattgccaa aagacggcaa tatggtggaa aataacgtcg actcatgtct gctcgaagcg
     9421 gccgcatcaa ccactttgta caagaaagct gggtcgaatt cgccctttta gctattctca
     9481 ctccactctg gcaccatgcc gacgccgtgt accgagtgat actgatgggg ggacagggtc
     9541 ctgggcacgc cgtgagtgta gagaaactct gggggctgga ggccgcccgg agactgaagg
     9601 ccagtctgag gcccgcactg cctgaccaca ggctgatggg ccactgaggt ctggtgctga
     9661 aacatccctt cgccgagctg gggagaacca tggttggcca tgccagccaa ccgagggacc
     9721 aggggccccg aggtgaaatg agcggagaag tgctggtagg gaagacggtc catgggctgc
     9781 acggtggtga cggtacagct gctataggag ggcatgctgg gccatgtgtt acagctgatg
     9841 tcctccaggc taggcacggg ctcgctgggg ggagcggagc tggcatacat gcaggcctgc
     9901 cgctgggccg actctgtcct gtaagaggta ctcaggccct gctgctgggg gtagcccgag
     9961 cgatagaagg tgtcttcctc gctgggggat gtctccatgt acggcttctt ataggggtgc
    10021 tccgtgctgg aacattcctc atctttcctc ttggtacagt ggtaaatttg gctgtgctcc
    10081 tgggccagtg ggtatgggtt aggtgggggc agaaggtcct gggaggggcc agagacacca
    10141 ttctcacact ggtactggga ccctaaattg gatgaggtgg agagagctcg ggtctcgctg
    10201 ctgaaggggc tgtggttgga ggtgactttg tgcctcattg tgctcctggg aaccacagga
    10261 tactctttac tttgcatccg agacatcctg tgtaactcca ggtcatcact gccccgaaag
    10321 cctttggcga aggggttgtt ctcaattttc agctgtgtga tcttgtgatt ctggtacgaa
    10381 gtcacagcga taaaagctgt ctccgggaag acgtgggtgc aaaacgcagt gttctttgaa
    10441 ccgaacccat tattttcgtc tgctttcacg atgtgtaatc ggggctggta tttgtgcatg
    10501 gagttcagga taatgtgtcc aaacgggtcc aggtggttgt tggtgagttt gagcttctgg
    10561 aaggagacaa gttgtcgcat ccagtgggct ccggttgctg gggagtccgg gtgcacgtaa
    10621 aggcgccccg gcatggcagg ctctgctttg ccagttacgg accatttgtt atcagcaaat
    10681 ttatatctgt ggtcgtctgc gggaacaata tccatgagaa gaatatactt cgttttggga
    10741 ttaaggccag tcaccttcac tttgtaacta ggaaacattc tcctccctgc cttggtgatg
    10801 atcatctctg tgcccacttc gtggaacttc agccacagtt cacgttcatg aagaaacacc
    10861 ttgattcctt ccatgccctg ctgggtgaag gcagcctgcg gggatgatgg agacttgctg
    10921 ggagccccca gagcactctc aggtttcgaa tcgcaagacc tgtctttgga atcaggctcc
    10981 agaggcgtgc gcgccaggcc aaagccctca tctgtatcgg ccataagggc gaattcggag
    11041 cctgcttttt tgtacaaact tgttgatgct agcaggctgg atcggtcccg gtgtcttcta
    11101 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct
    11161 ctgcttatat agggggtacc ttctctatca ctgataggga gctcgagacg tgtcgacttc
    11221 tctatcactg atagggagac gttctagac
//
