LOCUS       sequence-203625-        5880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adeno-Associated viral (AAV) vector plasmid expressing mouse 
            Beclin-1 (BECN1) in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Pircs K, Petri R, Madsen S, Brattas PL, Vuono R, Ottosson DR, 
            St-Amour I, Hersbach BA, Matusiak-Bruckner M, Lundh SH, Petersen A, 
            Deglon N, Hebert SS, Parmar M, Barker RA, Jakobsson J
  TITLE     Huntingtin Aggregation Impairs Autophagy, Leading to Argonaute-2 
            Accumulation and Global MicroRNA Dysregulation.
  JOURNAL   Cell Rep. 2018 Aug 7;24(6):1397-1406. doi: 
            10.1016/j.celrep.2018.07.017.
  PUBMED    30089251
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.07"; journalName: "Cell Rep"; date: 
            "2018-08-7- 7"; volume: "24"; issue: "6"; pages: "1397-1406"
COMMENT     Sequence Label: pAAV2-SBECN1W
FEATURES             Location/Qualifiers
     source          1..5880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        223..670
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    2079..2667
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2132..2152)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2550..2561)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2715..2849
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2752..2771)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2806..2825
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3286..3874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3366..3385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4045..4836,4837..4905))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4668..4687
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4906..5010)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5078..5096
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5134..5156)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5256..5275
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5342..5797)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5479..5500)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5691..5710
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
       61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
      121 gcgcgcagag agggagtggc caactccatc actaggggtt cctcagatcg ggctgcagga
      181 attggccgct ctagactcta gctgcagagg gacctgcgta tgagtgcaag tgggttttag
      241 gaccaggatg aggcggggtg ggggtgccta cctgacgacc gaccccgacc cactggacaa
      301 gcacccaacc cccattcccc aaattgcgca tcccctatca gagaggggga ggggaaacag
      361 gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc cttcgccccc
      421 gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg
      481 tcccccgcaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc
      541 cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg
      601 cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct
      661 gagagcgcag tcgaattcaa gctgctagca aggatccacc ggtgaggcat ggaggggtct
      721 aaggcgtcca gcagcaccat gcaggtgagc ttcgtgtgcc agcgctgtag ccagcctctg
      781 aaactggaca cgagcttcaa gatcctggac cgggtcacca tccaggaact cacagctcca
      841 ttacttacca cagcccaggc gaaaccagga gagacccagg aggaagaggc taactcagga
      901 gaggagccat ttattgaaac tcgccaggat ggtgtctctc gaagattcat ccccccagcc
      961 aggatgatgt ctacagaaag tgctaatagc ttcactctga tcggggaggc atctgatggt
     1021 ggcaccatgg agaacctcag ccggagactc aaggtcactg gagacctgtt tgacatcatg
     1081 tcgggccaga ccgatgtgga tcacccgctg tgtgaggaat gcacagacac tcttttagac
     1141 cagctggaca ctcagctcaa tgtcactgag aatgaatgtc agaactacaa acgctgtttg
     1201 gagatcctag agcagatgaa tgaggatgac agtgagcagc tgcagaggga gctgaaggag
     1261 ctggccttgg aggaggagag gctgatccag gagctggaag atgtggaaaa gaaccgcaag
     1321 gtggtggcag agaacctgga gaaggtccag gctgaggcgg agagattgga ccaggaggaa
     1381 gctcagtacc agcgggagta tagtgagttt aaaaggcagc agctggagtt ggatgacgaa
     1441 ctcaagagtg tggagaacca ggtgcgctac gcccagatcc agctggacaa gctcaagaaa
     1501 accaatgtct tcaatgccac cttccacatc tggcacagcg gacagtttgg cacaatcaat
     1561 aatttcagac tgggtcgctt gcccagtgtt cctgtggagt ggaatgaaat caatgctgcc
     1621 tggggccaga cagtgctgct gctccatgct ttggccaata agatgggtct gaagtttcag
     1681 aggtaccgac ttgttcccta tggaaatcat tcctatctgg agtctctgac agacaaatct
     1741 aaggagttgc cgttatactg ttctgggggt ttgcggtttt tctgggacaa caagtttgac
     1801 catgcaatgg tagcttttct ggactgtgtg cagcagttca aagaagaggt ggaaaaagga
     1861 gagactcgat tttgtcttcc gtacaggatg gacgtggaga aaggcaagat tgaagacact
     1921 ggaggcagtg gcggctccta ttccatcaaa acccagttta actcggagga gcagtggaca
     1981 aaagcgctca agttcatgct gaccaatctc aagtggggtc ttgcctgggt gtcctcacag
     2041 ttctataaca agtaaagctt actagtggat ccgtcgacaa tcaacctctg gattacaaaa
     2101 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     2161 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     2221 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     2281 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     2341 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     2401 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     2461 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     2521 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     2581 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     2641 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacagc ttatcgatac
     2701 cgtcgacttc gagcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2761 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2821 tcatcaatgt atcttatcat gtctggatcg tctagcatcg aagatccccc gatctgagga
     2881 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgc
     2941 ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
     3001 gcgcagagag ggagtggcca accccccccc ccccccccct gcagcccagc tgcattaatg
     3061 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3481 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3721 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4021 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4081 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4141 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4201 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4261 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4321 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4381 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4441 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4501 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4561 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4621 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4681 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4741 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4801 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4861 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4921 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4981 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     5041 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     5101 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     5161 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     5221 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     5281 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     5341 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     5401 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     5461 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     5521 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     5581 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     5641 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     5701 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     5761 acacccgccg cgcttaatgc gccgctacag ggcgcgtcgc gccattcgcc attcaggcta
     5821 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggctgca
//
