LOCUS       sequence-203626-       10873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of ilux for bioluminescence imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Gregor C, Gwosch KC, Sahl SJ, Hell SW
  TITLE     Strongly enhanced bacterial bioluminescence with the ilux operon for
            single-cell imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 
            10.1073/pnas.1715946115. Epub 2018 Jan 16.
  PUBMED    29339494
REFERENCE   2  (bases 1 to 10873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1715946115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-01-30- 30"; volume: "115"; issue: "5"; pages: "962-967"
COMMENT     Sequence Label: ilux pGEX(-)
FEATURES             Location/Qualifiers
     source          1..10873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(183..188,189..204,205..211)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..1700
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIITGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHDIVNFLYTVGQRWKNDEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGPKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWTIIESDAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1713..2636
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2691..3773
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMERLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGADMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESAATTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3788..4771
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINPTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPAEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNEDFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4956..6068
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     primer_bind     complement(6923..6945)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7090..7108)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7176..7280
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7281..7349,7350..8141)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7499..7518)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8312..8900
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8801..8820
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9054..9071
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        9144..9221
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9222..10304
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9241..9260)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    10317..10338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10353..10358,10359..10376,10377..10383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10391..10407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10415..10431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10415..10431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(10427..10873,1..81)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGITLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(10447..10464)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10447..10463)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10456..10478)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac aggatccatg actaaaaaaa tttcattcat tattaccggc caggttgaaa
      301 tctttcccga aagcgatgat ttagtgcaat ccattaattt tggtgataat agtgtttacc
      361 tgccaatatt gaatgactct catgtaaaaa acattattga ttgtaatgga aataacgaat
      421 tacggttgca tgacattgtc aattttctct atacggtagg gcaaagatgg aaaaatgatg
      481 aatactcaag acgcaggaca tacattcgtg acttaaaaaa atatatggga tattcagaag
      541 aaatggctaa gctagaggcc aattggatat ctatgatatt atgttctaaa ggcggccttt
      601 atgatgttgt agaaaatgaa cttggttctc gccatatcat ggatgaatgg ctacctcagg
      661 atgaaagtta tgttcgggct tttccgaaag gtaaatctgt acatctgttg gcaggtaatg
      721 ttccattatc tgggatcatg tctatattac gcgcaatttt aactaagaat cagtgtatta
      781 taaaaacatc gtcaaccgat ccttttaccg ctaatgcatt agcgttaagt tttattgatg
      841 tagaccctaa tcatccgata acgcgctctt tatctgttat atattggccc caccaaggtg
      901 atacatcact cgcaaaagaa attatgcgac atgcggatgt tattgtcgct tggggagggc
      961 cagatgcgat taattgggcg gtagagcatg cgccatctta tgctgatgtg attaaatttg
     1021 gtcctaaaaa gagtctttgc attatcgata atcctgttga tttgacgtct gcagcgacag
     1081 gtgcggctca tgatgtttgt ttttacgatc agcgagcttg tttttctgcc caaaacatat
     1141 attatatggg aaatcattat gaggaattta agttagcgtt gatagaaaaa cttaatctat
     1201 atgcgcatat attaccgaat gcaaaaaaag attttgatga aaaggcggcc tattctttag
     1261 ttcaaaaaga aagcttgttt gctggattaa aagtagaggt ggatattcat caacgttgga
     1321 cgattattga gtcagatgca ggtgtggaat ttaatcaacc acttggcaga tgtgtgtacc
     1381 ttcatcacgt cgataatatt gagcaaatat tgccttatgt tcaaaaaaat aagacgcaaa
     1441 ccatatctat ttttccttgg gagtcatcat ttaaatatcg agatgcgtta gcattaaaag
     1501 gtgcggaaag gattgtagaa gcaggaatga ataacatatt tcgagttggt ggatctcatg
     1561 acggaatgag accgttgcaa cgattagtga catatatttc tcatgaaagg ccatctaact
     1621 atacggctaa ggatgttgcg gttgaaatag aacagactcg tttcctggaa gaagataagt
     1681 tccttgtatt tgtcccataa taggtaaaaa gtatggaaaa tgaatcaaaa tataaaacca
     1741 tcgaccacgt tatttgtgtt gaaggaaata aaaaaattca tgtttgggaa acgctgccag
     1801 aagaaaacag cccaaagaga aagaatgcca ttattattgc gtctggtttt gcccgcagga
     1861 tggatcattt tgctggtctg gcggaatatt tatcgcggaa tggatttcat gtgatccgct
     1921 atgattcgct tcaccacgtt ggattgagtt cagggacaat tgatgaattt acaatgtcta
     1981 taggaaagca gagcttgtta gcagtggttg attggttaac tacacgaaaa ataaataact
     2041 tcggtatgtt ggcttcaagc ttatctgcgc ggatagctta tgcaagccta tctgaaatca
     2101 atgcttcgtt tttaatcacc gcagtcggtg ttgttaactt aagatattct cttgaaagag
     2161 ctttagggtt tgattatctc agtctaccca ttaatgaatt gccgaataat ctagattttg
     2221 aaggccataa attgggtgct gaagtctttg cgagagattg tcttgatttt ggttgggaag
     2281 atttagcttc tacaattaat aacatgatgt atcttgatat accgtttatt gcttttactg
     2341 caaataacga taattgggtc aagcaagatg aagttatcac attgttatca aatattcgta
     2401 gtaatcgatg caagatatat tctttgttag gaagttcgca tgacttgagt gaaaatttag
     2461 tggtcctgcg caatttttat caatcggtta cgaaagccgc tatcgcgatg gataatgatc
     2521 atctggatat tgatgttgat attactgaac cgtcatttga acatttaact attgcgacag
     2581 tcaatgaacg ccgaatgaga attgagattg aaaatcaagc aatttctctg tcttaaaatc
     2641 tattgagata ttctatcact caaatagcaa tataaggact ctctgaattc atgaaatttg
     2701 gaaacttttt acttacatac caacctcccc aattttctca aacagaggta atggaacgtt
     2761 tggttaaatt aggtcgcatc tctgaggagt gtggttttga taccgtatgg ttactggagc
     2821 atcatttcac ggagtttggt ctacttggta acccttatgt cgctgctgca tatttacttg
     2881 gcgcgactaa aaaattgaat gtaggaaccg ccgctattgt tcttcccaca gcccatccag
     2941 tacgccaact tgaagatgtg aatttattgg atcaaatgtc aaaaggacga tttcggtttg
     3001 gtatttgccg agggctttac aacaaggact ttcgcgtatt cggcgcggat atgaataaca
     3061 gtcgcgcctt agcggaatgc tggtacgggc tgataaagaa tggcatgaca gagggatata
     3121 tggaagctga taatgaacat atcaagttcc ataaggtaaa agtaaacccc gcggcgtata
     3181 gcagaggtgg cgcaccggtt tatgtggtgg ctgaatcagc tgcgacgact gagtgggcag
     3241 ctcaatttgg cctaccgatg atattaagtt ggattataaa tactaacgaa aagaaagcac
     3301 aacttgagct ttataatgag gtggctcaag aatatgggca cgatattcat aatatcgacc
     3361 attgcttatc atatataaca tctgtagatc atgactcaat taaagcgaaa gagatttgcc
     3421 ggaaatttct ggggcattgg tatgattctt atgtgaatgc tacgactatt tttgatgatt
     3481 cagaccaaac aagaggttat gatttcaata aagggcagtg gcgtgacttt gtattaaaag
     3541 gacataaaga tactaatcgc cgtattgatt acagttacga aatcaatccc gtgggaacgc
     3601 cgcaggaatg tattgacata attcaaaaag acattgatgc tacaggaata tcaaatattt
     3661 gttgtggatt tgaagctaat ggaacagtag acgaaattat tgcttccatg aagctcttcc
     3721 agtctgatgt catgccattt cttaaagaaa aacaacgttc gctattatat tagctaagga
     3781 gaaagaaatg aaatttggat tgttcttcct taacttcatc aatccgacaa ctgttcaaga
     3841 acaaagtata gttcgcatgc aggaaataac ggagtatgtt gataagttga attttgaaca
     3901 gattttagtg tatgaaaatc atttttcaga taatggtgtt gtcggcgctc ctctgactgt
     3961 ttctggtttt ctgctcggtt taacagagaa aattaaaatt ggttcattaa atcacatcat
     4021 tacaactcat catcctgtcc gcatagcgga ggaagcttgc ttattggatc agttaagtga
     4081 agggagattt attttagggt ttagtgattg cgaaaaaaaa gatgaaatgc atttttttaa
     4141 tcgcccggct gaatatcaac agcaactatt tgaagagtgt tatgaaatca ttaacgatgc
     4201 tttaacaaca ggctattgta atccagataa cgatttttat agcttcccta aaatatctgt
     4261 aaatccccat gcttatacgc caggcggacc tcggaaatat gtaacagcaa ccagtcatca
     4321 tattgttgag tgggcggcca aaaaaggtat tcctctcatc tttaagtggg atgattctaa
     4381 tgatgttaga tatgaatatg ctgaaagata taaagccgtc gcggataaat atgacgttga
     4441 cctatcagag atagaccatc agttaatgat attagttaac tataacgaag atagtaataa
     4501 agctaaacaa gagacgcgtg catttattag tgattatgtt cttgaaatgc accctaatga
     4561 agatttcgaa aataaacttg aagaaataat tgcagaaaac gctgtcggaa attatacgga
     4621 gtgtataact gcggctaaat tggcaattga aaagtgtggt gcgaaaagtg tattgctgtc
     4681 ctttgaacca atgaatgatt tgatgagcca aaaaaatgta atcaatattg ttgatgataa
     4741 tattaagaag taccacatgg aatataccta accatggtag atttcgagtt gcagcgaggc
     4801 ggcaagtgaa cgaatcccca ggagcataga taactatgtg actggggtga gtgaaagcag
     4861 ccaacaaagc agcagcttga aagatgaagg gtataaaaga gtatgacagc agtgctgcca
     4921 tactttctaa tattatcttg aggagtaaaa caggtatgac ttcatatgtt gataaacaag
     4981 aaattacagc aagctcagaa attgatgatt tgattttttc gagcgatcca ttagtgtggt
     5041 cttacgacga gcaggaaaaa atcagaaaga aacttgtgct tgatgcattt cgtaatcatt
     5101 ataaacattg tcgagaatat cgtcactact gtcaggcaca caaagtagat gacaatatta
     5161 cggaaattga tgacatacct gtattcccaa catcggtttt taagtttact cgcttattaa
     5221 cttctcagga aaacgagatt gaaagttggt ttaccagtag cggcacgaat ggtttaaaaa
     5281 gtcaggtggc gcgtgacaga ttaagtattg agagactctt aggctctgtg agttatggca
     5341 tgaaatatgt tggtagttgg tttgatcatc aaatagaatt agtcaatttg ggaccagata
     5401 gatttaatgc tcataatatt tggtttaaat atgttatgag tttggtggaa ttgttatatc
     5461 ctacgacatt taccgtaaca gaagaacgaa tagattttgt taaaacattg aatagtcttg
     5521 aacgaataaa aaatcaaggg aaagatcttt gtcttattgg ttcgccatac tttatttatt
     5581 tactctgcca ttatatgaaa gataaaaaaa tctcattttc tggagataaa agcctttata
     5641 tcataaccgg aggcggctgg aaaagttacg aaaaagaatc tctgaaacgt gatgatttca
     5701 atcatctttt atttgatact ttcaatctca gtgatattag tcagatccga gatatattta
     5761 atcaagttga actcaacact tgtttctttg aggatgaaat gcagcgtaaa catgttccgc
     5821 cgtgggtata tgcgcgagcg cttgatcctg aaacgttgaa acctgtacct gatggaacgc
     5881 cggggttgat gagttatatg gatgcgtcag caaccagtta tccagcattt attgttaccg
     5941 atgatgtcgg gataattagc agagaatatg gtaagtatcc cggcgtgctc gttgaaattt
     6001 tacgtcgcgt caatacgagg acgcagaaag ggtgtgcttt aagcttaacc gaagcgtttg
     6061 atagttgagt cgacctaagg agaaagaaat ggtgaagata cagcccatcc ccacaactag
     6121 ccagggcagc ctttttataa tgaatagcac catagagaca atcctgggcc atagatccat
     6181 taggaagttc acatctgaac ctattgctag tgagcagctg caaacgattc ttcagtctgg
     6241 gctcgctgct tcaagctcat ccatgctgca ggttgtgagt ataattcggg ttacagacac
     6301 ggaaaagaga aaattgctcg ctcaatatgc cggcaaccag acgtatgtgg aatccgctgc
     6361 tgagttcctg gtcttttgta tagactacca gcgacacgct actatcaacc ccgatgtcca
     6421 agctgacttt accgagctga ccctgattgg tgcagtggat tccggcataa tggcccagaa
     6481 ttgcctcctg gcagcagaat caatgggtct tggcggagtc tatatcggag gacttcggaa
     6541 ctcagctgcc caagtggatg agttgctcgg actgcccaag aacacagcta tcctcttcgg
     6601 aatgtgcttg gggcaccccg atcagagccc tgagacaaag cctagactgc ccgctcatgt
     6661 gatcgtgcac gagaaccaat atcaagctct gaacattgac gacgtacagg cgtatgacaa
     6721 aacattgcag gagtattatg ccagcagaac cagcaaccag aagcagagtg tctggtccca
     6781 ggaaactgca ggcaagctgg ccggagaatc cctcccacac atcctgccat acctgaactc
     6841 caagggcctt gccagaaggt aagcggccgc atcgtgactg actgacgatc tgcctcgcgc
     6901 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     6961 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7021 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat aattcttgaa
     7081 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7141 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7201 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7261 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7321 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7381 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7441 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7501 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     7561 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7621 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7681 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7741 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7801 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     7861 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7921 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7981 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8041 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8101 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8161 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8221 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8281 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8341 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8401 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     8461 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8521 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8581 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8641 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8701 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8761 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8821 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8881 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8941 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9001 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9061 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     9121 gtatttcaca ccgcataaat tccgacacca tcgaatggtg caaaaccttt cgcggtatgg
     9181 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat
     9241 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
     9301 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
     9361 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
     9421 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
     9481 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
     9541 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
     9601 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
     9661 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
     9721 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
     9781 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
     9841 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
     9901 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg
     9961 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
    10021 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgtcaa
    10081 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
    10141 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
    10201 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    10261 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    10321 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    10381 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
    10441 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
    10501 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    10561 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
    10621 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt
    10681 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac
    10741 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc
    10801 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga
    10861 tggcgttgga att
//
