LOCUS       sequence-203641-        8237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A Forster resonance energy transfer (FRET) biosensor for AMPK 
            activity with cyan and yellow fluorescent protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Konagaya Y, Terai K, Hirao Y, Takakura K, Imajo M, Kamioka Y, 
            Sasaoka N, Kakizuka A, Sumiyama K, Asano T, Matsuda M
  TITLE     A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous 
            AMPK Responses among Cells and Organs.
  JOURNAL   Cell Rep. 2017 Nov 28;21(9):2628-2638. doi: 
            10.1016/j.celrep.2017.10.113.
  PUBMED    29186696
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.10"; journalName: "Cell Rep"; date: 
            "2017-11-28- 28"; volume: "21"; issue: "9"; pages: "2628-2638"
COMMENT     Sequence Label: pPBbsr2-4031NES
FEATURES             Location/Qualifiers
     source          1..8237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     331..353
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     415..434
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(465..467,468..470,471..1178)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     CDS             1614..1961
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGXGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             join(2052..2054,2055..2057,2058..2570)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIGFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             2775..2801
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    2911..3460
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3055..3072)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3282..3301
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(3931..3948)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3937..4161
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4208..4224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4232..4248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4256..4262,4263..4280,4281..4286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4301..4322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   4556..4618
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4659..4679)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4659..4677)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4698..4714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4722..4738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4746..4752,4753..4770,4771..4776))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4791..4812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4929..4946)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5100..5688)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5180..5199)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5859..6650,6651..6719))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6482..6501
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6720..6824)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6850..7305)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6987..7008)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7199..7218
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7432..7454
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7446..7463
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7447..7463
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7473..7492
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7473..7491
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   7521..7555
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        7858..8237
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
       61 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
      121 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
      181 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
      241 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
      301 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
      361 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
      421 tgctggttat tgtgctgtct catcattttg gcaaagaatt cggcatggtg agcaagggcg
      481 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      541 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      601 agcttctatg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg
      661 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
      721 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      781 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      841 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      901 acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
      961 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
     1021 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     1081 ccgccctgtt caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcctga
     1141 ccgccgccgg gatcactgag ggcatgaacg agctgtacct cgagaagttt tctcaagaac
     1201 agatcggcga aaacattgtg tgcagggtca tttgtaccac gggtcaaatt cccatccgag
     1261 atttgtcagc tgatatttca caagtgctta aggaaaaacg atccataaag aaagtttgga
     1321 catttggtag aaacccagcc tgtgactatc atttaggaaa catttcaaga ctgtcaaata
     1381 agcatttcca aatactacta ggagaagacg gtaacctttt attgaatgac atttccacta
     1441 atgggacctg gttaaatggg caaaaagtcg agaagaacag caatcagtta ctgtcccaag
     1501 gtgatgaaat aaccgttggt gtaggcgtgg aatcagatat tttatctctg gtcattttca
     1561 taaacgacaa atttaagcag tgcctggagc agaacaaagt tgatcgcggt accagtgctg
     1621 gtggtagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggttccggca
     1681 gtgctggtgg tagtgctggt ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg
     1741 gtggtagtgc tggtggtagt gctggtggtt scggcagtgc tggtggtagt gctggtggta
     1801 gtaccagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg
     1861 gtggttccgg cagtgctggt ggtagtgctg gtggtagtac cagtgctggt ggtagtgctg
     1921 gtggtagtgc tggtggtagt gctggtggta gtgctggtgg ttccggaggc agcggcgagg
     1981 gcagcaccaa gatgaggagg gtggccaccc tggtggacct gggcaccggc ggcagcgagc
     2041 tgggcggccg catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2101 tcgagctgga cggcgacgta aacggccaca ggttcagcgt gtccggcgag ggcgagggcg
     2161 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2221 cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg
     2281 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2341 gtaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2401 gcgacaccct ggtgaaccgc atcgagctga agggcatcgg cttcaaggag gacggcaaca
     2461 tcctggggca caagctagag tacaactaca tcagccacaa cgtctatatc accgccgaca
     2521 agcagaagaa cggcatcaag gcccacttca agatccgcca caacatcgag gacggcggcg
     2581 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2641 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     2701 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2761 tgtctagact gcagctgcct cctctggaac gcctgactct ggattaagtc gagggccggc
     2821 gcgccgcggc cggccgcggt aacaattgtt aactaactta agctagcaac ggtttccctc
     2881 tagcgggatc aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag
     2941 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     3001 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     3061 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     3121 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     3181 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     3241 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     3301 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     3361 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     3421 accacgggga cgtggttttc ctttgaaaaa cacgataata ccatggtcat gaaaacattt
     3481 aacatttctc aacaagatct agaattagta gaagtagcga cagagaagat tacaatgctt
     3541 tatgaggata ataaacatca tgtgggagcg gcaattcgta cgaaaacagg agaaatcatt
     3601 tcggcagtac atattgaagc gtatatagga cgagtaactg tttgtgcaga agccattgcg
     3661 attggtagtg cagtttcgaa tggacaaaag gattttgaca cgattgtagc tgttagacac
     3721 ccttattctg acgaagtaga tagaagtatt cgagtggtaa gtccttgtgg tatgtgtagg
     3781 gagttgattt cagactatgc accagattgt tttgtgttaa tagaaatgaa tggcaagtta
     3841 gtcaaaacta cgattgaaga actcattcca ctcaaatata cccgaaatta aaagtcgaga
     3901 tgcatcgatg atctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     3961 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4021 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4081 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4141 gggatgcggt gggctctatg gcttctgagg cggaaagaac ctgcagccca agcttggcgt
     4201 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     4261 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     4321 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatct
     4381 taaaagtttt gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa
     4441 taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa
     4501 acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc
     4561 gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt
     4621 aatctagtat acgcgtttaa ttaatccagc ttttgttccc tttagtgagg gttaattgcg
     4681 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4741 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4801 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     4861 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     4921 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4981 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5041 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5101 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5161 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5221 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5281 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5341 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5401 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5461 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5521 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5581 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5641 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5701 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5761 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5821 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5881 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     5941 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6001 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6061 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6121 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6181 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6241 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6301 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6361 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6421 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6481 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6541 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6601 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6661 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6721 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6781 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6841 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     6901 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     6961 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     7021 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     7081 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     7141 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     7201 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     7261 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     7321 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     7381 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     7441 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
     7501 ggcgcgccat tctagattaa ccctagaaag atagtctgcg taaaattgac gcatgcattc
     7561 ttgaaatatt gctctctctt tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc
     7621 tcagtcgccg cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt
     7681 ttgaactata acgaccgcgt gagtcaaaat gacgcatgat tatcttttac gtgactttta
     7741 agatttaact catacgataa ttatattgtt atttcatgtt ctacttacgt gataacttat
     7801 tatatatata ttttcttgtt atagatatca actagaatgc tagcatgggc ccatctcgac
     7861 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     7921 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     7981 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     8041 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     8101 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     8161 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     8221 tcatcgctat taccatg
//
