LOCUS       sequence-203650-       12924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ratiometric BiFC analysis in plants (transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12924)
  AUTHORS   Grefen C, Blatt MR
  TITLE     A 2in1 cloning system enables ratiometric bimolecular fluorescence 
            complementation (rBiFC).
  JOURNAL   Biotechniques. 2012 Oct 2;53(5):311-14. doi: 10.2144/000113941.
  PUBMED    23066669
REFERENCE   2  (bases 1 to 12924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2012-10-2- 2"; volume: "53"; issue: "5"; 
            pages: "311-14"
COMMENT     Sequence Label: pBiFCt-2in1-NN
FEATURES             Location/Qualifiers
     source          1..12924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        93..437
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(531..533,534..536,537..995)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(576..597)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(837..856)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1029..1055
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1066..1189
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1224..1229,1230..1247,1248..1254)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1262..1278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1267..1289
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1286..1302
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1286..1302
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(1563..1687)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2014..2358
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2446..3120
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2446..3120
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     2844..2863
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3038..3061
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        3474..3818
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4097..4118
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4197..4226
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    4245..4369
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4394..4399,4400..4417,4418..4424)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4478..5137
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4544..4563)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5479..5784
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5663..5682
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5825..5949)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    6274..6298
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6819..7610
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7856..8444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8345..8364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8598..8615
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8630..8770
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8696..8715)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8856..8878
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9114..9308
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9374..10447)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10876..11505)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12836..12860
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 actagccctc tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc
       61 aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg
      121 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa
      181 aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
      241 gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa
      301 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
      361 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
      421 tttcatttgg agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca
      481 aacaacaaca acattacatt ttacattcta caactacatc tagtcaattg atggtgagca
      541 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      601 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      661 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      721 ccttcggcta cggcctgcag tgcttcgcca ggtaccccga ccacatgaag cagcacgact
      781 tcttcaagtc cgccatgccc gaaggctacg tccaggagag aaccatcttc ttcaaggacg
      841 acggcaacta caagaccagg gccgaggtga agttcgaggg cgacaccctg gtgaacagaa
      901 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      961 acaactacaa cagccacaac gtctatatca tggccggagg tggatctgga ggtggaagtc
     1021 agaacatgta tccttatgat gttcctgatt atgctagtca attggcaact ttgtataata
     1081 aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     1141 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcggg
     1201 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1261 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta ccccgtccct
     1321 gcacgcctgt cgttcaaccc tggaagatcc gcgtgtcccg agttctaaca gtctggccgt
     1381 cgttctgcag cgtcgcgatt gggaaaaccc gggcgtgacc caactgaatc gtctggcggc
     1441 ccatccgccg tttgcgtcgt ggcgtaactc agaagaagcc cgtaccgacc gtccgtctca
     1501 acaactgcgc tcctaacact ataaaaagct gatgcagacg gggctttttt ctgcaggtcg
     1561 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     1621 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     1681 aagtggtgtc taggttataa gcggccatgc tagagtccgc aaaaatcacc agtctctctc
     1741 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     1801 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     1861 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     1921 gtgacctagg agggctgtca acatggtgga gcacgacact ctcgtctact ccaagaatat
     1981 caaagataca gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc
     2041 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     2101 aaaggaagat ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     2161 tgcctctacc gacagtggtc ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa
     2221 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
     2281 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     2341 atttcatttg gagaggacct cgagcgcgcg tatttttaaa caattaccaa caacaacaac
     2401 aaacaacaac aacattacat tttacattct acaactacat ctagtatggc ctcctccgag
     2461 gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac
     2521 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     2581 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     2641 cagtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     2701 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     2761 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     2821 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     2881 tccaccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gatgaggctg
     2941 aagctgaagg acggcggcca ctacgacgcc gaggtcaaga ccacctacat ggccaagaag
     3001 cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc tggacatcac ctcccacaac
     3061 gaggactaca ccatcgtgga acagtacgag cgcgccgagg gccgccactc caccggcgcc
     3121 caattgttaa tcacaagttt gtacaaagtg gtgattaagc ggccatgcta gagtccgcaa
     3181 aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg
     3241 agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata
     3301 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     3361 tcctaaaacc aaaatccagt gaccttacct ctgcaggtca acatggtgga gcacgacact
     3421 ctcgtctact ccaagaatat caaagataca gtctcagaag accagagggc tattgagact
     3481 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
     3541 ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca ttgcgataaa
     3601 ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg acccccaccc
     3661 acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
     3721 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     3781 tcctctatat aaggaagttc atttcatttg gagaggacct cgagcgcgcg tatttttaaa
     3841 caattaccaa caacaacaac aaacaacaac aacattacat tttacattct acaactacat
     3901 ctaggatgga caagcagaag aacggcatca aggtgaactt caagatcagg cacaacatcg
     3961 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     4021 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     4081 acgagaagag agatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     4141 gcatggacga gctgtacaag tctggaggtg gatctggagg tggaagtagg ccaatggaac
     4201 agaagcttat ctcagaggag gacctggccg gcactagtcc tagaacaagt ttgtacaaaa
     4261 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     4321 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt
     4381 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
     4441 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     4501 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     4561 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     4621 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     4681 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     4741 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     4801 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     4861 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     4921 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     4981 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     5041 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     5101 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa
     5161 agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga
     5221 tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca
     5281 gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg
     5341 taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa
     5401 atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg
     5461 agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat
     5521 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc
     5581 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
     5641 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
     5701 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
     5761 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt
     5821 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     5881 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa cttttctata
     5941 caaagttggt caattgttat aagcggccat gctagagtcc gcaaaaatca ccagtctctc
     6001 tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa
     6061 gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta
     6121 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
     6181 cagtgaccta ggagggtaag tcctcaattc ggcgttaatt cagtacatta aaaacgtccg
     6241 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc
     6301 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
     6361 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat
     6421 gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc
     6481 tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaattc
     6541 gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     6601 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     6661 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     6721 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     6781 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg gtgatcgccg
     6841 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     6901 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     6961 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     7021 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     7081 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     7141 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     7201 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     7261 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     7321 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     7381 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     7441 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     7501 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     7561 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctagcta
     7621 gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact
     7681 aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc
     7741 gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa atcccttaac
     7801 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7981 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     8041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8221 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8461 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8521 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8581 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     8641 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     8701 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     8761 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8821 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8881 ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga
     8941 gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccctagg ccagccattt
     9001 ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg
     9061 accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac
     9121 aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc
     9181 cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg
     9241 ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc
     9301 tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca
     9361 tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca
     9421 tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat
     9481 ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac
     9541 tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg
     9601 ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg
     9661 tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga
     9721 tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg
     9781 cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg
     9841 caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc
     9901 catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg
     9961 gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca
    10021 tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta
    10081 cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca
    10141 gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca
    10201 tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac
    10261 cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt
    10321 caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact
    10381 tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac
    10441 cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac
    10501 ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc
    10561 ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact
    10621 catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg
    10681 taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca
    10741 actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct
    10801 tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct
    10861 ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc
    10921 gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc
    10981 tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag
    11041 cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc
    11101 cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc
    11161 ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt
    11221 gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg
    11281 ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag
    11341 cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac
    11401 taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt
    11461 cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt
    11521 atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac
    11581 tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc
    11641 ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt
    11701 gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct
    11761 tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt
    11821 ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg
    11881 caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca
    11941 gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt
    12001 cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact
    12061 tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac
    12121 gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg
    12181 tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg
    12241 caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa
    12301 cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc
    12361 cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt
    12421 tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc
    12481 tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa
    12541 aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg
    12601 acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
    12661 tatagcagcg gaggggttgg atcaaagtac tttaaagtac tttaaagtac tttaaagtac
    12721 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
    12781 cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta
    12841 cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct
    12901 gatccaagct caagctaagc tagg
//
