LOCUS       sequence-203653-        9389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM
  TITLE     A unifying model for mTORC1-mediated regulation of mRNA translation.
  JOURNAL   Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
  PUBMED    22552098
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-2- 2"; volume: "485"; issue: "7396"; pages:
            "109-13"
COMMENT     Sequence Label: pLJM60-eIF4E
FEATURES             Location/Qualifiers
     source          1..9389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2833..2877
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3184..3387
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3338..3358
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3384..3408
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3618..3636
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3618..3635
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     3649..3668
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3727..3750
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4483..4600
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4649..5159
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5078..5096
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5181..5780
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5181..5200)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5677..5697
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5908..6141
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6213..6334
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6250..6269)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6304..6323
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6374..6509
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6529..6548)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6530..6548)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6558..6575)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6558..6574)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6567..6589)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6716..7171
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6803..6822)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7013..7034
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7197..7301
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7302..7370,7371..8162)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7520..7539)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8333..8921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8822..8841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9075..9092
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9209..9230
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9245..9250,9251..9268,9269..9275)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9283..9299
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9288..9310
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9307..9323
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9307..9323
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9342..9362
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9344..9362
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaatact gatgaatccc ctaatgattt ttatcaaaat cattaagtta
     1501 aggtagatac acatcttgtc atatgatcaa atggtttcgc caaaaatcaa taatcagaca
     1561 acaaaatgtg cgaactcgat attttacacg actctcttta ccaattctgc cccgaattac
     1621 acttaaaacg actcaacagc ttaacgttgg cttgccacgc cttacttgac tgtaaaactc
     1681 tcactcttac cgaacttggc cgtaacctgc caaccaaagc gagaacaaaa cataacatca
     1741 aacgaatcga ccgattgtta ggtaatcgtc acctccacaa agagcgactc gctgtatacc
     1801 gttggcatgc tagctttatc tgttcgggca atacgatgcc cattgtactt gttgactggt
     1861 ctgatatccg tgagcaaaaa cggcttatgg tattgcgagc ttcagtcgca ctacacggtc
     1921 gttctgttac tctttatgag aaagcgttcc cgctttcaga gcaatgttca aagaaagctc
     1981 atgaccaatt tctagccgac cttgcgagca ttctaccgag taacaccaca ccgctcattg
     2041 tcagtgatgc tggctttaaa gtgccatggt ataaatccgt tgagaagctg ggttggtact
     2101 ggttaagtcg agtaagagga aaagtacaat atgcagacct aggagcggaa aactggaaac
     2161 ctatcagcaa cttacatgat atgtcatcta gtcactcaaa gactttaggc tataagaggc
     2221 tgactaaaag caatccaatc tcatgccaaa ttctattgta taaatctcgc tctaaaggcc
     2281 gaaaaaatca gcgctcgaca cggactcatt gtcaccaccc gtcacctaaa atctactcag
     2341 cgtcggcaaa ggagccatgg attctagcaa ctaacttacc tgttgaaatt cgaacaccca
     2401 aacaacttgt taatatctat tcgaagcgaa tgcagattga agaaaccttc cgagacttga
     2461 aaagtcctgc ctacggacta ggcctacgcc atagccgaac gagcagctca gagcgttttg
     2521 atatcatgct gctaatcgcc ctgatgcttc aactaacatg ttggcttgcg ggcgttcatg
     2581 ctcagaaaca aggttgggac aagcacttcc aggctaacac agtcagaaat cgaaacgtac
     2641 tctcaacagt tcgcttaggc atggaagttt tgcggcattc tggctacaca ataacaaggg
     2701 aagacttact cgtggctgca accctactag ctcaaaattt attcacacat ggttacgctt
     2761 tggggaaatt atgaggggat ctctcagagc ttaatacact ccttaattga agaatcgcaa
     2821 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2881 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2941 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     3001 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc ccatatgcca
     3061 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     3121 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     3181 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     3241 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     3301 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     3361 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     3421 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg
     3481 gaacggtgca ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga
     3541 gtctataggc ccaccccctt ggcttcgtta gaacgcggct acaattaata cataacctta
     3601 tgtatcatac acatacgatt taggtgacac tatagaataa catccacttt gcctttctct
     3661 ccacaggtgt ccactcccag gtccaactgc acctcggttc tatcgattga attcaccatg
     3721 gaaatggact acaaggatga cgatgacaag ggtggtggtg gtggtgcgtc gacgatggcg
     3781 actgtggaac cggaaaccac ccctaccact aatcccccac ctgcagaaga ggaaaaaaca
     3841 gagtctaatc aggaggttgc taacccagag cactatatta aacaccctct acagaacagg
     3901 tgggcactct ggttttttaa aaatgataaa agcaaaactt ggcaagcaaa ccttcgattg
     3961 atctctaagt ttgatactgt tgaagacttt tgggctctat acaaccatat ccagttgtct
     4021 agtaatttaa tgcctggctg tgactactca ctttttaagg acgggattga gcctatgtgg
     4081 gaagatgaga aaaacaaacg aggaggacgg tggctgatca cactgaacaa gcagcagaga
     4141 cggagtgacc tcgatcgctt ctggctagag acactgctgt gccttattgg agaatctttc
     4201 gatgactaca gtgatgatgt gtgtggagct gttgttaatg ttagagctaa aggtgataag
     4261 atagcaatat ggactactga gtgtgaaaac agagatgcag tcacacacat agggagggta
     4321 tacaaggaaa ggttaggact tcctccgaag atagtgattg gttatcagtc ccacgcagac
     4381 acagctacaa agagcggctc taccactaaa aataggtttg ttgtttaagc ggccgcagtg
     4441 agaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     4501 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     4561 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     4621 cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc
     4681 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     4741 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     4801 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     4861 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     4921 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     4981 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     5041 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     5101 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     5161 gggatccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     5221 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     5281 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     5341 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     5401 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     5461 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     5521 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     5581 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     5641 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     5701 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     5761 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     5821 cacgacccca tgcatcggta cctttaagac caatgactta caaggcagct gtagatctta
     5881 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5941 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6001 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6061 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6121 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     6181 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     6241 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6301 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     6361 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6421 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6481 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     6541 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6601 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6661 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     6721 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6781 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6841 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6901 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6961 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7021 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7081 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7141 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     7201 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7261 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7321 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7381 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7441 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7501 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7561 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7621 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7681 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7741 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7801 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7861 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7921 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7981 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8041 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8101 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8161 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8221 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8281 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8341 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8401 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8461 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8521 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8581 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8641 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8701 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8761 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8821 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8881 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8941 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9001 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     9061 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     9121 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     9181 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9241 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9301 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     9361 gggaacaaaa gctggagctg caagcttaa
//
