LOCUS       sequence-203659-       12932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ratiometric BiFC analysis in plants (transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12932)
  AUTHORS   Grefen C, Blatt MR
  TITLE     A 2in1 cloning system enables ratiometric bimolecular fluorescence 
            complementation (rBiFC).
  JOURNAL   Biotechniques. 2012 Oct 2;53(5):311-14. doi: 10.2144/000113941.
  PUBMED    23066669
REFERENCE   2  (bases 1 to 12932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2012-10-2- 2"; volume: "53"; issue: "5"; 
            pages: "311-14"
COMMENT     Sequence Label: pBiFCt-2in1-CC
FEATURES             Location/Qualifiers
     source          1..12932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        93..437
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    532..655
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(690..695,696..713,714..720)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    728..744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     733..755
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     752..768
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     752..768
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(1029..1153)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1170..1196
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1230..1232,1233..1235,1236..1694)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(1275..1296)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1536..1555)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        2021..2365
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2453..3127
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2453..3127
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     2851..2870
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3045..3068
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        3482..3826
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    3920..4044
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4069..4074,4075..4092,4093..4099)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4153..4812
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4219..4238)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5154..5459
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5338..5357
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5500..5624)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5641..5670
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     5898..5919
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6282..6306
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6827..7618
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7864..8452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8353..8372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8606..8623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8638..8778
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8704..8723)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8864..8886
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9122..9316
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9382..10455)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10884..11513)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12844..12868
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 actagccctc tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc
       61 aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg
      121 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa
      181 aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
      241 gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa
      301 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
      361 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
      421 tttcatttgg agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca
      481 aacaacaaca acattacatt ttacattcta caactacatc tagtcaattg gcaactttgt
      541 ataataaagt tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
      601 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
      661 cgcgggctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      721 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacccc
      781 gtccctgcac gcctgtcgtt caaccctgga agatccgcgt gtcccgagtt ctaacagtct
      841 ggccgtcgtt ctgcagcgtc gcgattggga aaacccgggc gtgacccaac tgaatcgtct
      901 ggcggcccat ccgccgtttg cgtcgtggcg taactcagaa gaagcccgta ccgaccgtcc
      961 gtctcaacaa ctgcgctcct aacactataa aaagctgatg cagacggggc ttttttctgc
     1021 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     1081 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     1141 tgtacaaagt ggtgtctagg ttaaacatgt atccttatga tgttcctgat tatgctacta
     1201 gtggaggtgg atctggaggt ggaagtagaa tggtgagcaa gggcgaggag ctgttcaccg
     1261 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1321 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1381 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt
     1441 gcttcgccag gtaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1501 aaggctacgt ccaggagaga accatcttct tcaaggacga cggcaactac aagaccaggg
     1561 ccgaggtgaa gttcgagggc gacaccctgg tgaacagaat cgagctgaag ggcatcgact
     1621 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1681 tctatatcat ggcctgatta taagtaagcg gccatgctag agtccgcaaa aatcaccagt
     1741 ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca
     1801 gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     1861 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     1921 aaatccagtg acctaggagg gctgtcaaca tggtggagca cgacactctc gtctactcca
     1981 agaatatcaa agatacagtc tcagaagacc agagggctat tgagactttt caacaaaggg
     2041 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga
     2101 cagtagaaaa ggaagatggc ttctacaaat gccatcattg cgataaagga aaggctatcg
     2161 ttcaagatgc ctctaccgac agtggtccca aagatggacc cccacccacg aggaacatcg
     2221 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
     2281 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     2341 gaagttcatt tcatttggag aggacctcga gcgcgcgtat ttttaaacaa ttaccaacaa
     2401 caacaacaaa caacaacaac attacatttt acattctaca actacatcta gtatggcctc
     2461 ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa
     2521 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     2581 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     2641 tcagttccag tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     2701 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     2761 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     2821 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     2881 ggaggcctcc accgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagat
     2941 gaggctgaag ctgaaggacg gcggccacta cgacgccgag gtcaagacca cctacatggc
     3001 caagaagccc gtgcagctgc ccggcgccta caagaccgac atcaagctgg acatcacctc
     3061 ccacaacgag gactacacca tcgtggaaca gtacgagcgc gccgagggcc gccactccac
     3121 cggcgcccaa ttgttaatca caagtttgta caaagtggtg attaagcggc catgctagag
     3181 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata
     3241 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt
     3301 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     3361 ttctaattcc taaaaccaaa atccagtgac cttaccctct gcaggtcaac atggtggagc
     3421 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta
     3481 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
     3541 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt
     3601 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac
     3661 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     3721 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
     3781 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg agcgcgcgta
     3841 tttttaaaca attaccaaca acaacaacaa acaacaacaa cattacattt tacattctac
     3901 aactacatct agtcctagaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
     3961 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     4021 aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat
     4081 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
     4141 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     4201 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     4261 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     4321 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     4381 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     4441 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     4501 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     4561 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     4621 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     4681 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     4741 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     4801 ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     4861 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     4921 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     4981 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     5041 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     5101 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
     5161 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     5221 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     5281 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     5341 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     5401 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     5461 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     5521 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     5581 atatcatttt acgtttctcg ttcaactttt ctatacaaag ttggtcaatt gttaccaatg
     5641 gaacagaagc ttatctcaga ggaggacctg gccggcacta gtggaggtgg atctggaggt
     5701 ggaagtatgg acaagcagaa gaacggcatc aaggtgaact tcaagatcag gcacaacatc
     5761 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5821 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     5881 aacgagaaga gagatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5941 ggcatggacg agctgtacaa gtagttataa gcggccatgc tagagtccgc aaaaatcacc
     6001 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc
     6061 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
     6121 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     6181 ccaaaatcca gtgacctagg agggtaagtc ctcaattcgg cgttaattca gtacattaaa
     6241 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     6301 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     6361 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     6421 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     6481 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     6541 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     6601 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     6661 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     6721 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     6781 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
     6841 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     6901 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     6961 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     7021 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     7081 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     7141 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     7201 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     7261 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     7321 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     7381 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     7441 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     7501 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     7561 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     7621 tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     7681 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     7741 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat
     7801 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7861 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7921 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7981 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     8041 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8101 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8161 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8221 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8281 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8341 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8401 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8461 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8521 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8581 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     8641 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     8701 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     8761 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     8821 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     8881 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
     8941 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccctaggcc
     9001 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
     9061 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
     9121 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
     9181 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
     9241 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
     9301 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
     9361 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
     9421 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
     9481 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
     9541 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
     9601 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
     9661 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
     9721 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
     9781 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
     9841 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
     9901 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
     9961 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
    10021 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
    10081 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
    10141 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
    10201 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
    10261 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
    10321 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
    10381 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
    10441 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
    10501 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
    10561 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
    10621 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
    10681 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
    10741 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
    10801 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
    10861 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
    10921 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
    10981 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
    11041 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
    11101 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
    11161 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
    11221 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
    11281 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
    11341 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
    11401 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
    11461 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
    11521 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
    11581 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
    11641 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
    11701 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
    11761 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
    11821 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
    11881 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
    11941 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
    12001 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
    12061 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
    12121 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
    12181 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
    12241 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
    12301 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
    12361 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
    12421 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
    12481 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
    12541 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
    12601 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
    12661 gactgcacta tagcagcgga ggggttggat caaagtactt taaagtactt taaagtactt
    12721 taaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
    12781 ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct
    12841 taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac
    12901 gacaatctga tccaagctca agctaagcta gg
//
