LOCUS       sequence-203661-       13247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13247)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETtv-2in1-CC
FEATURES             Location/Qualifiers
     source          1..13247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        143..487
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    581..705
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(730..735,736..753,754..760)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             814..1473
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(880..899)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1815..2120
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1999..2018
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2161..2285)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2305..2307,2308..2310,2311..3024)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        3374..3718
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    3813..3936
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3971..3976,3977..3994,3995..4001)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4009..4025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4014..4036
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4033..4049
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4033..4049
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4310..4434)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(4454..4456,4457..4459,4460..5173)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        5499..5677
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6290..6542
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6647..6671
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7192..7983
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8229..8817
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8718..8737
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8971..8988
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9003..9143
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9069..9088)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9229..9251
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9487..9681
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9747..10820)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11249..11878)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13209..13233
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagccctc
       61 tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
      121 tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
      181 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg
      241 gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg
      301 acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc
      361 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      421 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      481 agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca aacaacaaca
      541 acattacatt ttacattcta caactacatc tagtcctaga acaagtttgt acaaaaaagc
      601 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
      661 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
      721 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
      781 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      841 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      901 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      961 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     1021 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     1081 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     1141 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     1201 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     1261 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     1321 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1381 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1441 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc
     1501 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     1561 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     1621 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     1681 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     1741 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     1801 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     1861 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     1921 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     1981 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     2041 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     2101 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     2161 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     2221 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt tctatacaaa
     2281 gttggtcaat tgttaggcac tagtatggtg agcaagggcg aggagctgtt caccggggtg
     2341 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     2401 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc
     2461 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc
     2521 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2581 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2641 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2701 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2761 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     2821 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2881 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag caaggatccc
     2941 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3001 ggcatggacg agctgtacaa gtaattataa gcggccatgc tagagtccgc aaaaatcacc
     3061 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc
     3121 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
     3181 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     3241 ccaaaatcca gtgacctagg agggctcgca cgtgcggagc tccgtaccct ctgcaggtca
     3301 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     3361 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     3421 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
     3481 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
     3541 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
     3601 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     3661 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacct
     3721 cgagcgcgcg tatttttaaa caattaccaa caacaacaac aaacaacaac aacattacat
     3781 tttacattct acaactacat ctagtcaatt ggcaactttg tataataaag ttgaacgaga
     3841 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     3901 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcgggctc actcattagg
     3961 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     4021 aacaatttca cacaggaaac agctatgacc atgattaccc cgtccctgca cgcctgtcgt
     4081 tcaaccctgg aagatccgcg tgtcccgagt tctaacagtc tggccgtcgt tctgcagcgt
     4141 cgcgattggg aaaacccggg cgtgacccaa ctgaatcgtc tggcggccca tccgccgttt
     4201 gcgtcgtggc gtaactcaga agaagcccgt accgaccgtc cgtctcaaca actgcgctcc
     4261 taacactata aaaagctgat gcagacgggg cttttttctg caggtcgacc atagtgactg
     4321 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     4381 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgtctag
     4441 gttaggcact agtatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4501 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     4561 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4621 gccctggccc accctcgtga ccaccctgtc ctggggcgtg cagtgcttcg cccgctaccc
     4681 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4741 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4801 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4861 catcctgggg cacaagctgg agtacaacta ctttagcgac aacgtctata tcaccgccga
     4921 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg
     4981 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     5041 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     5101 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     5161 gctgtacaag taattataag cggccatgct agagtccgca aaaatcacca gtctctctct
     5221 acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg
     5281 aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     5341 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     5401 tgacctagga gggtagatca tgagcggaga attaagggag tcacgttatg acccccgccg
     5461 atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc
     5521 actgagcgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc
     5581 gcgttcaaaa gtcgcctaag gtcactatca tctagcaaat atttcttgtc aaaaatgctc
     5641 cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc actctcaact
     5701 cgatcgaggg gatctaccat gagtccagaa aggagaccag cagatattag gagagcaacc
     5761 gaagcagata tgccagcagt ttgcaccatt gtgaaccatt acatcgagac ttctacagtt
     5821 aattttagga ctgaacctca agagccacag gaatggacag atgatctcgt gagattaagg
     5881 gaaagatacc cttggcttgt tgctgaggtg gatggagaag ttgcaggtat tgcttacgca
     5941 ggaccttgga aggctagaaa cgcttatgat tggactgctg agtctaccgt ttacgtgtca
     6001 cctagacatc aaagaaccgg acttggttca accttgtata ctcacctttt gaagtctctt
     6061 gaagctcagg gattcaaatc tgttgtggct gttatcggtt tgcctaatga tccaagtgtg
     6121 agaatgcatg aagctctcgg atacgcacca aggggtatgt taagagctgc tggatttaaa
     6181 catggtaact ggcacgatgt tggtttctgg cagttagatt tcagtttacc agttccacca
     6241 agaccagtgc ttccagtgac cgagatttga cccgggctag agtcaagcag atcgttcaaa
     6301 catttggcaa taaagtttct taatattgaa tcctgttgcc ggtcttgcga tgattatcat
     6361 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     6421 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     6481 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     6541 tcgaccggca tgaagctgag ttaacgatgt aagtcctcaa ttcggcgtta attcagtaca
     6601 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     6661 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     6721 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     6781 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     6841 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     6901 ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     6961 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     7021 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     7081 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     7141 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
     7201 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     7261 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     7321 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     7381 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     7441 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     7501 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     7561 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     7621 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     7681 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     7741 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     7801 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     7861 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     7921 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     7981 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     8041 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     8101 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc
     8161 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8221 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8281 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8341 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     8401 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8461 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8521 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8581 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8641 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8701 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8761 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8821 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8881 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8941 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9001 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     9061 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     9121 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     9181 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     9241 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
     9301 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccct
     9361 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
     9421 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
     9481 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
     9541 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
     9601 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
     9661 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca
     9721 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
     9781 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
     9841 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
     9901 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
     9961 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
    10021 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
    10081 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
    10141 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
    10201 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
    10261 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
    10321 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
    10381 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
    10441 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
    10501 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
    10561 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
    10621 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
    10681 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
    10741 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
    10801 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
    10861 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
    10921 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
    10981 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
    11041 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
    11101 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
    11161 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
    11221 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
    11281 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
    11341 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
    11401 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
    11461 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
    11521 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
    11581 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
    11641 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
    11701 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
    11761 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
    11821 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
    11881 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
    11941 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
    12001 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
    12061 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
    12121 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
    12181 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
    12241 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
    12301 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
    12361 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
    12421 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
    12481 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
    12541 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
    12601 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
    12661 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
    12721 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
    12781 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
    12841 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
    12901 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
    12961 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
    13021 aagccgactg cactatagca gcggaggggt tggatcaaag tactttaaag tactttaaag
    13081 tactttaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
    13141 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
    13201 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagttt
//
