LOCUS       sequence-203727-        8079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retrovirally express mouse MYB-del(TAD) with 3*FLAG tag at N 
            terminal and puro resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Xu Y, Milazzo JP, Somerville TDD, Tarumoto Y, Huang YH, Ostrander 
            EL, Wilkinson JE, Challen GA, Vakoc CR
  TITLE     A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute 
            Myeloid Leukemia.
  JOURNAL   Cancer Cell. 2018 Jan 8;33(1):13-28.e8. doi: 
            10.1016/j.ccell.2017.12.002.
  PUBMED    29316427
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2017.12"; journalName: "Cancer Cell"; date: 
            "2018-01-8- 8"; volume: "33"; issue: "1"; pages: "13-28.e8"
COMMENT     Sequence Label: MSCV-puro-N3FLAG-MYB-del(TAD)
FEATURES             Location/Qualifiers
     source          1..8079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             120..719
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(120..139)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     616..636
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             819..1333
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    1495..1511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1519..1525,1526..1543,1544..1549))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1564..1585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1702..1719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1873..2461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1953..1972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2632..3423,3424..3492))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3255..3274
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3493..3597)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3665..3683
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3721..3743)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3843..3862
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4056..4078
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4206..4225
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             4425..4941
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    5005..5346
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             5413..5829
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5759..5781
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5797..5813
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             5848..5868
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5869..5934
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(7741..7764)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
       61 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
      121 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
      181 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
      241 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
      301 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
      361 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
      421 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
      481 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
      541 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
      601 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
      661 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
      721 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgataa
      781 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
      841 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat
      901 agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata
      961 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     1021 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     1081 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     1141 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
     1201 ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     1261 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     1321 gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg agggtaggag
     1381 tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat actccaatac
     1441 tcctgaaata gttcattatg gacagcgcag aaagagctgg ggagaattgt gaaattgtta
     1501 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1561 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1621 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1681 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1741 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1801 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1861 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1921 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1981 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2041 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2101 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2161 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2221 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2281 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2341 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2401 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2461 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2521 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2581 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2641 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2701 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2761 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2821 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2881 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2941 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3001 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3061 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3121 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3181 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3241 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3301 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3361 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3421 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3481 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3541 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3601 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     3661 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     3721 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     3781 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     3841 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     3901 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     3961 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     4021 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     4081 ggcgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     4141 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     4201 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     4261 gccacgatgc gtccggcgta gaggcgatta gtccaatttg ttaaagacag gatatcagtg
     4321 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
     4381 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     4441 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac
     4501 tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa
     4561 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     4621 gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     4681 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     4741 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc
     4801 cagtcctccg atagactgcg tcgcccgggt acccgtattc ccaataaagc ctcttgctgt
     4861 ttgcatccga atcgtggact cgctgatcct tgggagggtc tcctcagatt gattgactgc
     4921 ccacctcggg ggtctttcat ttggaggttc caccgagatt tggagacccc tgcccaggga
     4981 ccaccgaccc ccccgccggg aggtaagctg gccagcggtc gtttcgtgtc tgtctctgtc
     5041 tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc gtctgtacta gttagctaac
     5101 tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc cggccgcaac
     5161 cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg aggaagggag
     5221 tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga gaacctaaaa
     5281 cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg cgcgtcttgt
     5341 ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg
     5401 tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa
     5461 gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc
     5521 ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac
     5581 cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca
     5641 gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc
     5701 aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc
     5761 cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct
     5821 ctaggcgccg gaattagatc tgccatgcca aagaagaagc gtaaggtaga ttacaaagac
     5881 catgacggtg attataaaga tcatgatatc gattacaagg atgacgatga caagggaggc
     5941 ctcgaggccc ggagaccccg acacagcatc tacagtagcg atgaagatga tgaagacatt
     6001 gagatgtgtg accatgacta cgatgggctg ctgcccaaat ctggaaagcg tcacttgggg
     6061 aaaactaggt ggacaaggga agaggatgag aagctgaaga agctggtgga acagaacgga
     6121 acagacgact ggaaagtcat tgccaattat ctgcccaacc ggacagatgt gcagtgccaa
     6181 caccggtggc agaaagtgct gaaccctgaa ctcatcaaag gtccctggac caaagaagaa
     6241 gatcagagag tcatagagct tgtccagaaa tatggtccga agcgttggtc tgttattgcc
     6301 aagcacttaa aagggagaat tggaaagcag tgtcgggaga ggtggcacaa ccatttgaat
     6361 ccagaagtta agaaaacctc ctggacagaa gaggaggaca gaatcattta ccaggcacac
     6421 aagcgtctgg ggaacagatg ggcagagatc gcaaagctgc tgcccggacg gactgataat
     6481 gctatcaaga accactggaa ttccaccatg cgtcgcaagg tggaacagga aggctacctg
     6541 caggagcctt ccaaagccag ccagacgcca gtggccacga gcttccagaa gaacaatcat
     6601 ttgatggggt ttgggcatgc ctcacctcca tctcagctct ctccaagtgg ccagtcctcc
     6661 gtcaacagcg aatatcccta ttaccacatc gccgaagcac aaaacatctc cagtcacgtt
     6721 ccctatcctg tcgcattgca tgttaatata gtcaacgtcc ctcagcacag cacctccatt
     6781 gtggaccaga ccagacctca tggggatagt gcacctgttt cctgtttggg agaacaccat
     6841 gccaccccat ctctgcctgc agatcccggc tccctacctg aagaaagtgc ctcaccagca
     6901 aggtgcatga tcgtccacca gggcaccatt ctggacaatg ttaagaacct cttagaattt
     6961 gcagaaacac tccagtttat agattctttc ttgaacactt ccagcaacca tgaaagctcg
     7021 ggcttagatg cacctacctt accctccact cctctcattg gtcacaaact gacaccatgt
     7081 cgagaccaga ctgtgaaaac ccagaaggaa aattccatct ttagaactcc agctatcaaa
     7141 aggtcaatcc tcgaaagctc tcctcgaact cccacaccat tcaaacatgc ccttgcagct
     7201 caagaaatta aatacggtcc cctgaagatg ctacctcaga ccccctccca tgcagtggag
     7261 gacctacaag atgtgattaa gcaggaatcg gatgaatctg gaattgtggc tgagtttcaa
     7321 gagagtggac caccgttact gaaaaaaatc aagcaggagg tggagtcgcc aactgagaaa
     7381 tcgggaaact tcttctgctc aaaccactgg gcagagaaca gcctgagcac ccagctgttc
     7441 tcgcaggcgt ctcctgtggc agatgcccca aatattctta caagctctgt tttaatgaca
     7501 cctgtatcag aagatgaaga caatgtcctc aaagccttta ccgtacctaa gaacaggccc
     7561 ctggtgggtc ccttgcagcc atgcagtggt gcctgggagc cagcatcctg tgggaagaca
     7621 gaggaccaga tgacggcctc cggtccggct cggaaatacg tgaacgcgtt ctcagctcga
     7681 actctggtca tgtgagaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     7741 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     7801 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     7861 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     7921 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     7981 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     8041 ttctgggctc agaggctggg aaggggtggg tccgggggc
//
