LOCUS       sequence-203745-        9259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Controller plasmid [ca. 558.5 relative RBS strength at PLas81*].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Boehm CR, Grant PK, Haseloff J
  TITLE     Programmed hierarchical patterning of bacterial populations.
  JOURNAL   Nat Commun. 2018 Feb 22;9(1):776. doi: 10.1038/s41467-018-03069-3.
  PUBMED    29472537
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-02-22"; volume: "9(1):776. doi"; pages: " 
            10.1038/s41467-018-03069-3"
COMMENT     Sequence Label: pCRB DRT7VTPLux*500
FEATURES             Location/Qualifiers
     source          1..9259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      852..923
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      939..966
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    975..991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    998..1014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1806..1852
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1894..1921
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2575..2586
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2593..2595,2596..2598,2599..3312)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      3332..3403
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3419..3446
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3606..4421)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3606..4421)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4329..4348
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4413..4432
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    4608..4629
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      complement(4636..4663)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(4679..4750)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(4770..5483,5484..5486,5487..5489))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     RBS             5496..5507
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     rep_origin      complement(8158..8702)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8238..8257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9170..9188
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(9192..9235)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    9238..9259
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatacta
       61 gagtcacaca ggactactag atgaaaaaca tcaacgcgga cgacacctac cgtatcatta
      121 acaaaatcaa agcgtgtcgt tctaacaacg atattaacca atgcctgtct gatatgacca
      181 aaatggttca ctgtgaatat tatctgctgg ccatcattta tccgcacagc atggtaaaat
      241 ccgatatttc tattctggat aactacccaa aaaaatggcg tcagtactat gatgacgcca
      301 acctgatcaa gtacgatccg atcgtagatt actctaactc taaccattcc ccgattaact
      361 ggaatatctt tgagaacaac gcggttaaca aaaagtcccc aaacgttatc aaagaagcaa
      421 aaaccagcgg tctgatcacc ggtttcagct tcccgattca taccgcgaac aacggcttcg
      481 gtatgctgtc ttttgcgcac tccgaaaaag acaactacat cgattctctg ttcctgcacg
      541 cgtgcatgaa cattcctctg atcgttccgt ccctggttga taactatcgc aagattaaca
      601 ttgccaataa caaaagcaac aacgacctga ccaaacgcga gaaagaatgc ctggcatggg
      661 cgtgcgaagg taagtcctct tgggacatct ccaaaattct gggttgctct gaacgcaccg
      721 taactttcca tctgaccaac gcgcagatga aactgaacac cacgaatcgt tgtcaatcta
      781 tcagcaaagc tatcctgacc ggtgcgatcg attgcccgta tttcaaaaac taataactaa
      841 tggccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      901 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      961 tctgcgttta taaattgtga gcggataaca attgacattg tgagcggata acaagatact
     1021 gagcacatac tagagggata ttaactaatt taacggcatc acgagtaatg gctctggtgg
     1081 atggttttct ggaactggaa cgttcttctg gtaaactgga atggagcgct atcctgcaaa
     1141 aaatggcctc tgacctgggt ttctctaaaa tcctgttcgg cctgctgccg aaagattccc
     1201 aagactacga aaacgcattc atcgtaggca attacccggc cgcgtggcgt gaacattatg
     1261 atcgtgccgg ttatgcacgt gtagatccaa ccgtttccca ttgcacccag tccgtgctgc
     1321 cgattttctg ggaaccgagc atctaccaga cccgtaaaca gcacgagttc ttcgaagagg
     1381 cgtccgcggc tggtctggtt tacggcctga cgatgccgct gcatggtgct cgtggcgaac
     1441 tgggcgctct gtctctgtcc gtagaagcag aaaaccgcgc ggaggctaac cgtttcatcg
     1501 aatctgtact gccgaccctg tggatgctga aagattatgc actgcaatcc ggcgcgggtc
     1561 tggcgttcga acacccagtt tctaaaccgg tagttctgac cagccgtgaa aaagaggtcc
     1621 tgcaatggtg cgctatcggc aaaacctcct gggagatttc tgtaatttgc aactgctctg
     1681 aagccaacgt caacttccac atgggtaaca ttcgtcgtaa attcggtgtt acttctcgcc
     1741 gcgttgcagc gattatggcc gttaacctgg gcctgattac tctgtaataa gcatgcacca
     1801 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1861 tgtcggtgaa acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1921 gtttatatac tagagtgagc tcgacctata ggatcgtata ggtttacgca agaaaatggt
     1981 ttgttacttt cgaataaata ctagagtcac acaggactac tagatgaaca cgattaacat
     2041 cgctaagaac gacttctctg acatcgaact ggctgctatc ccgttcaaca ctctggctga
     2101 ccattacggt gagcgtttag ctcgcgaaca gttggccctt gagcatgagt cttacgagat
     2161 gggtgaagca cgcttccgca agatgtttga gcgtcaactt aaagctggtg aggttgcgga
     2221 taacgctgcc gccaagcctc tcatcactac cctactccct aagatgattg cacgcatcaa
     2281 cgactggttt gaggaagtga aagctaagcg cggcaagcgc ccgacagcct tccagttcct
     2341 gcaagaaatc aagccggaag ccgtagcgta catcaccatt aagaccactc tggcttgcct
     2401 aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc gcaatcggtc gggccattga
     2461 ggacgaggct cgcttcggtc gtatccgtga ccttgaagct aagcacttca agaaaaacgt
     2521 tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag taataatact agagaaagag
     2581 gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     2641 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     2701 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
     2761 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
     2821 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     2881 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2941 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3001 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     3061 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     3121 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     3181 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     3241 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     3301 ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     3361 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     3421 ctcaccttcg ggtgggcctt tctgcgttta tatactagag atgcaggctt cctcgctcac
     3481 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3541 aatctcgagt cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat
     3601 tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     3661 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     3721 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     3781 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     3841 acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca
     3901 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     3961 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     4021 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     4081 ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat
     4141 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     4201 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     4261 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     4321 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     4381 cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
     4441 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
     4501 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
     4561 aaggatcaga tcacctacgt aattagttag ttagccctta gtgactcgaa ttcgcggccg
     4621 cttctagagt ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga
     4681 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     4741 gttttatttg atgcctggct ctagtattat tacttgtaca gctcgtccat gccgagagtg
     4801 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     4861 ttgctcagct tggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     4921 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg
     4981 tggcggatct tgaagttggc cttgatgccg ttcttctgct tgtcggcggt gatatagacg
     5041 ttgtcgctaa agtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     5101 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     5161 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     5221 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac
     5281 tgcacgcccc aggacagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     5341 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
     5401 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     5461 gtgaacagct cctcgccctt gctcaccatc tagtatttct cctctttctc tagtattatt
     5521 acgcgaacgc gaagtccgac tctaagatgt cacggaggtt caagttacct ttagccggaa
     5581 gtgctggcat tttgtccaat tgagactcgt gcaactggtc agcgaactgg tcgtagaaat
     5641 cagccagtac atcacaagac tcatatgtgt caaccatagt ttcgcgcact gctttgaaca
     5701 ggttcgcagc gtcagccgga atggtaccga aggagtcgtg aatcagtgca aaagattcga
     5761 ttccgtactt ctcgtgtgcc cacactacag tcttacgaag gtggctaccg tcttggctgt
     5821 gtacaaagtt aggagcgata ccagactcct gtttgtgtgc atcaatctcg ctatctttgt
     5881 tggtgttaat ggtaggctgt aagcggaact gaccgaggaa catcaggttc aagcgcgtct
     5941 gaataggctt cttgtattcc tgccacacag ggaaaccatc aggagttacc caatgcacag
     6001 cgcaacgctt gcgaagaatc tctccagtct tcttatcttt gacctcagca gccagcagct
     6061 tagcagcaga cttaagccag ttcattgctt caaccgcagc taccaccgtc acgctcacag
     6121 attcccaaat cagcttagcc atgtatccag cagcctgatt cggctgagtg aacatcagac
     6181 ccttgccgga atcaatagct ggctgaatgg tatcttccag cacttgttga cggaagccga
     6241 actctttgga cccgtaagcc agcgtcatga ctgaacgctt agtcacactg cgagtaacac
     6301 cgtaagccag ccattgacca gccagtgcct tagtgcccag cttgactttc tcagagattt
     6361 caccagtgtt ctcatcggtc acggtaacta cttcgttatc ggtcccattg attgcgtctg
     6421 cttgtagaat ctcgttgact ttcttagcaa caatcccgta gatgtcctga acggtttcac
     6481 taggaagcaa gttaaccgcg cgaccaccta cctcatctcg gagcatcgcg gagaagtgct
     6541 ggatgccaga gcaagacccg tcaaacgcca gcggaaggga gcagttatag ctcaggccgt
     6601 ggtgctgtac cccagcgtac tcaaagcaga acgcaaggaa gcagaacgga gaatcttgct
     6661 cagcccacca agtgttctcc agtggagact tagcgcaagc catgatgttc tcgtggtttt
     6721 cctcaatgaa cttgatgcgc tcagggaacg gaaccttatc gacacccgca cagtttgcac
     6781 cgtggatttt cagccagtag taaccttcct taccgattgg tttacctttc gccagcgtaa
     6841 gcagtccttt ggtcatatcg ttaccttgcg ggttgaacat tgacacagcg taaacacgac
     6901 cgcgccagtc catgttgtaa gggaaccaga tggccttatg gttagcaaac ttattggctt
     6961 gctcaagcat gaactcaagg ctgatacggc gagacttgcg agccttgtcc ttgcggtaca
     7021 cagcagcggc agcacgtttc cacgcggtga gagcctcagg attcatgtcg atgtcttccg
     7081 gtttcatcgg gagttcttca cgctcaatcg cagggatgtc ctcgaccgga caatgcttcc
     7141 acttggtgat tacgttggcg accgctagga ctttcttgtt gattttccat gcggtgtttt
     7201 gcgcaatgtt aatcgctttg tacacctcag gcatgtaaac gtcttcgtag cgcatcagtg
     7261 ctttcttact gtgagtacgc accagcgcca gaggacgacg accgttagcc caatagccac
     7321 caccagtaat gccagtccac ggcttaggag gaactacgca aggttggaac atcggagaga
     7381 tgccagccag cgcacctgca cgggttgcga tagcctcagc gtattcaggt gcgagttcga
     7441 tagtctcaga gtcttgacct actacgccag cattttggcg gtgtaagcta accattccgg
     7501 ttgactcaat gagcatctcg atgcagcgta ctcctacatg aatagagtct tccttatgcc
     7561 acgaagacca cgcctcgcca ccgagtagac ccttagagag catgtcagcc tcgacaactt
     7621 gcataaatgc tttcatctag tagtcctgtg tgactctagt atttattcga aagtaacaaa
     7681 ccattttctt gcgtaaactt gtacgatcct acaggtgcat cttcccgaca acgcagaccg
     7741 ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat
     7801 caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcct ggtatgagtc
     7861 agcaacacct tcttcacgag gcagacctca gcgctagcgg agtgtatact ggcttactat
     7921 gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac
     7981 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
     8041 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
     8101 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
     8161 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     8221 aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct cgtgcgctct
     8281 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     8341 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     8401 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8461 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
     8521 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
     8581 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
     8641 cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc
     8701 aagaagatca tcttattaag gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8761 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8821 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8881 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8941 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9001 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9061 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attccatggt
     9121 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     9181 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
     9241 ttcgcggccg cttctagag
//
