LOCUS       sequence-203755-        9400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock inducible transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Dzafic E, Strzyz PJ, Wilsch-Brauninger M, Norden C
  TITLE     Centriole Amplification in Zebrafish Affects Proliferation and 
            Survival but Not Differentiation of Neural Progenitor Cells.
  JOURNAL   Cell Rep. 2015 Oct 6;13(1):168-182. doi: 
            10.1016/j.celrep.2015.08.062. Epub 2015 Sep 24.
  PUBMED    26411683
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.08.062"; journalName: "Cell Rep"; date: 
            "2015-10-6- 6"; volume: "13"; issue: "1"; pages: "168-182"
COMMENT     Sequence Label: HS-DN-Ect2
FEATURES             Location/Qualifiers
     source          1..9400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2864..2888
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2891..2893,2894..2896,2897..3607)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2891..2893,2894..2896,2897..3607)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2936..2957)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3197..3216)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3544..3565
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4626..4650)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4655..4789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4679..4698)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4733..4752
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6930..7117,7118..7129)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7163..7179)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7163..7179)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7216..7236)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7216..7234)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7255..7271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7255..7271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7268..7290)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7279..7295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7303..7309,7310..7327,7328..7333))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7348..7369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7486..7503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7657..8245)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7737..7756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8416..9207,9208..9276))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9039..9058
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9277..9381)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttgcttcag gggtgtcgct tggttatttc caaaaatcaa
     1381 attaatttta ttaaactatt agaacgagca tgttttgtct atatgctaca gaagataaaa
     1441 aataatagga gttaacagtt ataaaacaac acactttgtt tctattgatt gttgaccaca
     1501 ctggggtctc attaagttag attaaagaca cactaactgg gtcaaaagca gcagattgat
     1561 ttcatagcac cagggtaaac tttctaacac ttttacggca atcatataca ttaaaattaa
     1621 atacagacca cgactgaaca aggaggatga tctccaatat taaacaaaga gacttgtgcc
     1681 tatttctctg agggtaaaca tgacctctca agttagcaag ttggttttaa cactacaaaa
     1741 atagttaaga ctgcaatccc agaataaagt attggtttta accaatcaat atagtacagt
     1801 aaacatccat ttgttttgtt gaaacgttaa acaaatctga ccaaagctat tagcttatat
     1861 aaaacaggtt tgccttctat gtagctgaaa acaccacagg cccgattttg ctactgtgta
     1921 aaacatttca gcaagatttt tttattgcat ttttttttac tgaatcgttc aaacatttta
     1981 tcattttagt ttgttcattc attgcaactg gaaaaacaac acatcacaca accgcacata
     2041 tttcagcaat aagtacaata aaacactcaa ataaaaaaaa cattttaaat ctctttgtat
     2101 ttttgaccgc tgtttcgcgt aatttcacgg taaaactctg gaaatctcca ctacattcct
     2161 ctcagcggct cctctcaatg acagctgaag aagtgacgcg gctgcctgct gtgttttgat
     2221 tggtcgaatt cactggaggc ttccagaaca gtgtagagtc tgaacgggtg cgcgctctgc
     2281 tgtatttaaa gggcgaaaga gagaccgcag agaaactcaa ccgaagagaa gcgacttgac
     2341 aaagaagaaa agagcagcct gacaggactt tttccccgac gaggtgttta ttcgctctat
     2401 ttaagaatct actgtaaggt aagtctcaat atattgtact ctattggcta atcagaatta
     2461 tatagagatt atatgtactt aatgtcaaaa aatcaacttt gtatatgtaa tctttttaca
     2521 tgtggactgc ctatgttcat cttattttag gtctactaga aaattatatt tcccgttttc
     2581 acaataagga tttttaaaaa aagcaatgaa cagacgggca tttactttat gttgctgaca
     2641 ttattttata tgagcataat aaccataaat actagcaaat gtcctaaatg aatttgtgtt
     2701 aatgttgtct acaaaagaaa attagcgttt tacttgtaca actaataata acttggttat
     2761 taagagaatt tcacttgttg actagaaaaa tcctttcata atgaaacaat tgcaccataa
     2821 attgtataaa tataaaatta attctaattg tttttttttc ctgccaagtt tgtacaaaaa
     2881 agcaggctgg atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2941 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     3001 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3061 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     3121 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3181 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3241 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3301 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3361 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3421 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3481 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     3541 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     3601 gtacaagtcc ggactcagat ccatggctga aaatagtgta ttaacatcca ctactgggag
     3661 gactagcttg gcagactctt ccatttttga ttctaaagtt actgagattt ccaaggaaaa
     3721 cttacttatt ggatctactt catatgtaga agagatgcct cagattgaaa caagagtgat
     3781 attggttcaa gaagctggaa aacaagaaga acttataaaa gccttaaagg acattaaagt
     3841 gggctttgta aagatggagt cagtggaaga atttgaaggt ttggattctc cggaatttga
     3901 aaatgtattt gtagtcacgg actttcagga ttctgtcttt aatgacctct acaaggctga
     3961 ttgtagagtt attggaccac cagttgtatt aaattgttca caaaaaggag agcctttgcc
     4021 attttcatgt cgcccgttgt attgtacaag tatgatgaat ctagtactat gctttactgg
     4081 atttaggaaa aaagaagaac tagtcaggtt ggtgacattg gtccatcaca tgggtggagt
     4141 tattcgaaaa gactttaatt caaaagttac acatttggtg gcaaattgta cacaaggaga
     4201 aaaattcagg gttgctgtga gtctaggtac tccaattatg aagccagaat ggatttataa
     4261 agcttgggaa aggcggaatg aacaggattt ctatgcagca gttgatgact ttagaaatga
     4321 atttaaagtt cctccatttc aagattgtat tttaagtttc ctgggatttt cagatgaaga
     4381 gaaaaccaat atggaagaaa tgactgaaat gcaaggaggt aaatatttac cgcttggaga
     4441 tgaaagatgc actcaccttg tagttgaaga gaatatagta aaagatcttc cctttgaacc
     4501 ttcaaagaaa ctttatgttg tcaagcaaga gtggttctgg ggaagcattc aaatggatgc
     4561 ccgagctgga gaaactatgt atttatatga aaaggcaaat actcctgagc tcaagaaatc
     4621 ataggaccca gctttcttgt acaaagttga tgtagatcca gacatgataa gatacattga
     4681 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     4741 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     4801 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc
     4861 cgcggcgcca atgcattggg ccctcgagca tatgagatct gtcccgatct ttctcttctg
     4921 tgctgtcaat ttagagtagt ttttgaggta atttgggtgc attctctgta aaaacaacaa
     4981 ataaatattt atattgtatg caataaatgt aacaattaca catacactct ctgattacag
     5041 cattaaaggg ttagttcacc caaaaatgaa aagatgtcat taatgactca ccctcatgtc
     5101 gttccaagcc cgtaacacct ccgttcatct tcggaacaca gtttaagata ttttagattt
     5161 agtccgagag ctttctgtcc ctccactgaa aatgtatgta cggtatactg tccatgtcca
     5221 gaaaggtaat aaaaacatca aagtagtcca tgtgacatca gtgggttagg tagaattatt
     5281 tgaagcatcg aaaatacatt ttggtccaaa aataacaaaa cctacgactt tattcagcat
     5341 tgtagcgtca ctgcgaagtc gtgaacgcgg attgacaaca gacccggaag agaatacaat
     5401 gccgaataaa gtcgtaggtt ttgttatttt tggaccaaaa tgtattcgat gcttcaaaaa
     5461 attctaacta acccactgat gtcacatgga ctactttgat gtttttatta cctttctgga
     5521 catggacagt ataccgtaca tacattctca atggaggcac agaaagctct cggactaaat
     5581 ctaaaatatc ttaaactgtg ttctgaagat gaacggaggt cttacgggct tggaacgaca
     5641 tgagggcgag tcattaatga cattattttc atttttgggt gaactaaccc tttaataaaa
     5701 ctgacatttg cagccaattg cacactttat aatgttaaat acttgttgat tttagaaata
     5761 cagtagcatt tagtgacaat caaacattaa ttttatttct gattatattg acaaaaacac
     5821 aggactgtaa ggctgacatg acagggtgaa ttgatttcat tacattagtt ttgatggtaa
     5881 cacttttaca ataaggttcg ttagttaact acattagtta acatgaacta ataatgaact
     5941 gcacgtatac atgttaattt caacatttac taatacttta tatcttgtta acattagtta
     6001 atgcactgtg aactaacatg aacaaacaat gaacatctgt atttctatta actaacgtta
     6061 acaaagatta acagatacag taacaaatgt attgctcatg gttatttaat gttagttaat
     6121 acatgaacta atcaacctca ttgtaaagtg ttaccgtttt gacaatacaa catgtttgga
     6181 aaaaaatttc tcgcttggtt ttcttgtgtt ttgcatgaat tcaaacacaa tgagagcacg
     6241 gaatgataat gattattcaa agctgcaata ggcgccaaat ttcccgtgga cctaacgtta
     6301 ccaattacaa atgcatttta gcattttatt acgtgagcct tatattgtca atcgcatgca
     6361 ttagtgaaaa taggcctact acacatataa aaggcgcgcc atcaacgtca aaacatgcac
     6421 acccaaaaaa aaaaaaaaaa aaaaaaaaaa aaactatcag taaaacaaaa tacttaatgt
     6481 acttacctca atatgcttcc ttaggtttga tggcgaactt ttgaaggccg atatttcttt
     6541 attaagcggg agacagagga cacatttcat cttgaatgaa tctttattta caccacttaa
     6601 agaaaagaat tcgcgaagat acggccacgg gtgctcttga tcctgtggct gattttggac
     6661 tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac
     6721 tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca
     6781 tgacgtcact tccaaaggac caatgaacat gtctgaccaa tttcatataa tgtgaaaacg
     6841 attttcatag gcagaataaa taacatttaa attaaactgg gcatcagcgc aattcaattg
     6901 gtttggtaat agcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga
     6961 ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt
     7021 aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat
     7081 acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg ttcttgaccc
     7141 cctaccttca gcaagcccag cagatccact agttctagag cggccgccac cgcggtggag
     7201 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     7261 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7321 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7381 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7441 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7501 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7561 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7621 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7681 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7741 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7801 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7861 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7921 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7981 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8041 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8101 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8161 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8221 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8281 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8341 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8401 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8461 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8521 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8581 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8641 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8701 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8761 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8821 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8881 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8941 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9001 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9061 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9121 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9181 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9241 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9301 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9361 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
//
