LOCUS       sequence-203760-       13151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transgenesis, doublecortin DCX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13151)
  AUTHORS   Icha J, Kunath C, Rocha-Martins M, Norden C
  TITLE     Independent modes of ganglion cell translocation ensure correct 
            lamination of the zebrafish retina.
  JOURNAL   J Cell Biol. 2016 Oct 24;215(2):259-275.
  PUBMED    27810916
REFERENCE   2  (bases 1 to 13151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-10-24"; volume: "215(2)"; pages: "259-275"
COMMENT     Sequence Label: ath5:GFP-DCX
FEATURES             Location/Qualifiers
     source          1..13151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1346..1364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1389..1405
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1390..1406
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1440..1456)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6387..6403)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6440..6458)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    6458..6482
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(6485..6487,6488..6490,6491..7201)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6485..6487,6488..6490,6491..7201)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6530..6551)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6791..6810)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7138..7159
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7229..7246
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7256..7273
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(8376..8400)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8405..8539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8429..8448)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8483..8502
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(10681..10868,10869..10880)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10914..10930)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(10967..10985)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11006..11022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11006..11022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11019..11041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11030..11046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11054..11060,11061..11078,11079..11084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11099..11120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11237..11254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11408..11996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11488..11507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12167..12958,12959..13027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12790..12809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13028..13132)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttggtaata cgactcacta tagggcgaat tgggtaccgg
     1381 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     1441 atccactagt tctagaattt aaatatttta aaaatgtata tatataataa atgtatatat
     1501 atataaatgt ataatgagca aaattctaag ttttatgcaa ataaaaaaag tattatggaa
     1561 aatgtattat attgcagttc taaattattt aagaaaacgt ggctgacttg ccaaaatgat
     1621 ggtaaactgt gcaactctgc ttccaagatg ttagaaacag acgtttttct tttagcctca
     1681 taatttaatg ctgttgcgat gttaccgtct cttcgctgta ctggttgtta ccaggttacg
     1741 gaaaaatata acatgctcac aacatccaat cagattaaat gaactgaaaa gcaatatggg
     1801 ttttaagaca tcacagagaa ggtaacattt aaaggcttaa aagcacaaac tgtttctcgc
     1861 ttctaagact aagtaagtaa gtttttgcac acaattaaca aatagttgca ggcaaattaa
     1921 actttagtga caaggcagta ctggcccgat caggccagta atgattttgt ctactgtcca
     1981 aagcatctct cacgctggcc ctgggtcatc aagcagttct tattgttgag ccctgcatta
     2041 taattatata caattttttt tttattattt aattttttta aattaaaatg ttatgtaatg
     2101 ttatgttaat aaatgtttaa ataagaatat taaaaaataa tttgatttaa tacaaataca
     2161 gggaagcacg ttttaaaaag taaataaatt ttgttaaatt ttatataagg aaaatatgtt
     2221 tatttctgta tttttctata cactgaacac aactaagaat ttctaaataa atatagacaa
     2281 atgctattag gcatgggatg ataaccagtt taaaggtcaa ggtttaaaaa cccccagaat
     2341 tatctgttat accgttccaa cgatatatgt aattttttta ttaacacttt taataaaaaa
     2401 ggaccctcat catcaaactg ttcttaaaat aaactatatt gtgctcaaag gtaaaaacag
     2461 ttcttgtttt ttacccagac atttaaaaaa agaatttatt ttagagcagt aatcacaata
     2521 ccgtccgtga taccgtgatt tttttttatc aaaggtaatc atacagtcac aatcttttac
     2581 cggcccatgc tatttatacg gcagtgtaaa ataaagtgta aattgtaacg cgttataaag
     2641 aattgcttct tttaaacaat gccacaaaaa ttatgaacat tttttttttg taaaaatttt
     2701 aaaccaaaaa taactaaaaa tttctccaaa tataaaacaa ttgagcaggg cacattgtaa
     2761 cacataacga aaactgcttc cattgttttt ttttatactt gaaaatcctt ttcatctttg
     2821 tcccacataa gtcataacat taacaataat aattgaatat agatttcttg tctaattgac
     2881 tgcccttaat aagacattgc ttcattgacc agttaagatt aaacataaca accagaaggg
     2941 aacaaaaagg ctcaaatagg catgaaatta caagcacctt gcttgaccta attacagtga
     3001 cgagcctcat ctcaccagat gccccaatct gacggtgggg gttcaagtct cctcatctat
     3061 ggaggtcact gtttacacaa tatgcctcac ttttctgaat gacctcattg gtcaatatgc
     3121 cagttctgtg agaatcgatc aaaaatagct ttaaagatta aagattttaa gttttcttct
     3181 cactttttta ggtagattat gtgttgaaat gaacacaaca gtttataccc atatctcaga
     3241 gatcatatcg atgatgaact ttaatgaatg ataagttatt atattatata ttaaatcata
     3301 ttatattata atatattata ttctatattc gttttgatag atgtagtgtt taaatgaaca
     3361 gtttatactc atctcaagga tcataaagaa gtgtcatatc atagaactgt aaatgacaat
     3421 gttatattat tttgatggac ctttagatat attctttggt tattatactg tatatttttg
     3481 gtagattatg tttacatcat agagctttaa atgactagtt ctattctagt ctatttgatt
     3541 tttttttttt taaatgacca caacagttta tactcaaata tcagtgatca taatgttata
     3601 tcaacatata aaatctatat tatgttatat tatattatat tatattatat tatattatat
     3661 tatattttat tttatattag acagcaccca ttctcagttt tacatctatt atttgactgt
     3721 cagcttgact taagtaggag tttgatcatt agtcgtcact ttcctcgagt tcagaggatt
     3781 acgcattcga tttttaagac cattttctcc ttcggaatga acggcgcttt atgctttaaa
     3841 tgaaaaccag atttactaaa tcctggatta agctgttaaa ctgataatag accttctcac
     3901 tttcccctcg tcaagaccga gtaaatcttt aatctgctca acactgtctc aaaaattaca
     3961 ggagtagctt gtaacatatg gccttttgca taggagtcca gacaagccta tcatgtgaac
     4021 gagtgaaaaa acaaggccgt ttaacatgtc tggaccaagc cctttaaacg ggacaagcag
     4081 cagctggtca gggatgccaa tctgcaatca atgaaaacaa actaaaccag tggattgggt
     4141 ttatatgccc aattgaacag cagaattact catctaatgc agatgagcgt atatggcttg
     4201 atggccacag tcagccggga catgaattat gcagaagaga tatagcgatt tgtcacttgt
     4261 acacaaatca gatgtaataa tgtatgtatt tagtgcagta ttttatatag aacgctactt
     4321 tacgatttca agtacctgta ggaaaatatg ttatagataa ttactatact atagaggtga
     4381 taattcataa tgcataactt taaaaacatt ttgtttcata gtatagcaat tatatataca
     4441 gtatataaga tagactgcat atatatatat atatatatat attatatctg tgtgttatta
     4501 tattataacc ttagtataac aataatagta ttttggaaag gtttatgaat gagttttttt
     4561 gccaacaggc tttttttgga gggggtgaaa ctgtgactgt ctgaatctgc tttgtttgat
     4621 tcacattgaa cagattgaga tcgacagctt gtcgaagact tctgtctgtc tcaattcctc
     4681 tgagacactc aaatcaatta tttagtgctg ccccacaaat gtgttttata tggttttgtt
     4741 attggtttac tatatattat taaatatata gaatgtaata tgtaatatag agaaatgtgc
     4801 tgatgataca gtagatgaat actgacacta tattaaaata ttctatataa aagtataaaa
     4861 tttgaacact gacgctatat taaaagattg caaatgaccg tataaatgtt taaaatgtag
     4921 aaacttcaca tgttattttt tctaaacaag catgctggtt attactttat aattatgtaa
     4981 caatataact gtactaatac agttcagtaa ttaacaagta attcaaaaat atttttattt
     5041 taaatgttta tattttatca ttattcttca agccactagg ttatattagt ttgtcagcaa
     5101 aaatgaacaa attagcacca acgcttataa tttaaaatat taaatcttga aaatatttta
     5161 aagaatgtta catttaatga aaattatttt aaaaagcata agaaaaatta cacactcaaa
     5221 ttgcatttaa tatgctttcc gtgtaaatta cttatgtaaa tttaatttga tgcacactaa
     5281 aatgtctcgt ctatgaccta tatacataat gtattatcat attgtaactc aaatattaca
     5341 tgtaatatta atattacctt aaatatttat atattacaat ttaacattat gaataacaaa
     5401 gacaactgga gtatgtttga ccaaaacaaa acaacaatct ttctatttgt aaaagttaat
     5461 tttaaaaaaa ctgttgcgat gtgtaaaata tccatttact ttattcagta aaactaatgt
     5521 ggattataat agtatagcat aaatatataa caatatatca atttaatatt aacatcaata
     5581 taaaattgat gaatctatct atgaaaaaat atgaaaaatt cattatttac tcacttatta
     5641 ctaattgcac acccgtatac tattcttttt tcattgaaca caaagaaaga tatgttgaag
     5701 tatgctgaaa accggtaacc attgacttca acagtattta tttttccaat ggcagccagt
     5761 ggttacaggt ttgcagcttt caaccaaata ttttcttttg tgatcagcag aagaaagaaa
     5821 ctcataaaag tttggaacca cttaaataaa atagtgcata aatagtctgt aaatgttcat
     5881 ttttgtatga actatccctt caaattatcg tttatattaa aagattccag taataaatgt
     5941 aaataatgag acctacaacc ctgtaaaaca aattgcaaaa tcaaactgac tctatacagg
     6001 caattactcc tgcaaacaat gttctcattt aagaaatcca aagatgtcac ggttgcttaa
     6061 agatgcatgc actttttaat taggtaatgg tccttgccac ccggcgctgg ttgtggccag
     6121 atggtaagtt atctctggtt tgtaacgcct gttttcagtg atagagcagc caaagttaat
     6181 tggaatgtca ttaaagtttg agttactggg ggttggggct ggactgcact agtcctgctg
     6241 ttggagatgt gcgtttcatt aataataatg gatgtttgga tggagtaata agggtggggc
     6301 tccacctgct gccacctgtt aatactctat ataagcatcg ctcatcctta tggcagaaca
     6361 gaagcgatta agaaccatcc gggggggatc cactagttct agagcggccg ccaccgcggt
     6421 ggagctccag cttttgttcc ctttagtgag ggttaattca agtttgtaca aaaaagcagg
     6481 ctggatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     6541 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     6601 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     6661 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     6721 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     6781 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     6841 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     6901 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     6961 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     7021 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     7081 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     7141 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     7201 gtccggactc agatccatgg gcagcagcca tcatcatcat catcacagca gcggcctggt
     7261 gccgcgcggc agccatatgg aacttgattt tggacacttt gacgaaagag ataagacatc
     7321 caggaacatg cgaggctccc ggatgaatgg gttgcctagc cccactcaca gcgcccactg
     7381 tagcttctac cgaaccagaa ccttgcaggc actgagtaat gagaagaaag ccaagaaggt
     7441 acgtttctac cgcaatgggg accgctactt caaggggatt gtgtacgctg tgtcctctga
     7501 ccgttttcgc agctttgacg ccttgctggc tgacctgacg cgatctctgt ccgacaacat
     7561 caacctgcct cagggagtgc gatacattta caccattgat ggatccagga agatcggaag
     7621 catggatgaa ctggaggaag gggaaagcta tgtctgttcc tcagacaact tctttaaaaa
     7681 ggtggagtac accaagaatg tcaatcccaa ctggtctgtc aacgtaaaaa catctgccaa
     7741 tatgaaagcc ccccagtctt tggctagcag caacagtgca caggccaggg agaacaagga
     7801 ctttgtgcgc cccaagctgg ttaccatcat ccgcagtggg gtgaagcctc ggaaggctgt
     7861 gcgtgtgctt ctgaacaaga agacagccca ctcttttgag caagtcctca ctgatatcac
     7921 agaagccatc aaactggaga ccggggttgt caaaaaactc tacactctgg atggaaaaca
     7981 ggtaacttgt ctccatgatt tctttggtga tgatgatgtg tttattgcct gtggtcctga
     8041 aaaatttcgc tatgctcagg atgatttttc tctggatgaa aatgaatgcc gagtcatgaa
     8101 gggaaaccca tcagccacag ctggcccaaa ggcatcccca acacctcaga agacttcagc
     8161 caagagccct ggtcctatgc gccgaagcaa gtctccagct gactcaggta acgaccaaga
     8221 cgcaaacgga acctccagca gccagctctc tacccccaag tctaagcagt ctcccatctc
     8281 tacgcccacc agtcctggca gcctccggaa gcacaaggac ctgtacctgc ctctgtcctt
     8341 ggatgactcg gactcgcttg gtgattccat gtaagaccca gctttcttgt acaaagttga
     8401 tgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     8461 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     8521 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     8581 ggaggtgtgg gaggtttttt aattcgcggc cgcggcgcca atgcattggg ccctcgagca
     8641 tatgagatct gtcccgatct ttctcttctg tgctgtcaat ttagagtagt ttttgaggta
     8701 atttgggtgc attctctgta aaaacaacaa ataaatattt atattgtatg caataaatgt
     8761 aacaattaca catacactct ctgattacag cattaaaggg ttagttcacc caaaaatgaa
     8821 aagatgtcat taatgactca ccctcatgtc gttccaagcc cgtaacacct ccgttcatct
     8881 tcggaacaca gtttaagata ttttagattt agtccgagag ctttctgtcc ctccactgaa
     8941 aatgtatgta cggtatactg tccatgtcca gaaaggtaat aaaaacatca aagtagtcca
     9001 tgtgacatca gtgggttagg tagaattatt tgaagcatcg aaaatacatt ttggtccaaa
     9061 aataacaaaa cctacgactt tattcagcat tgtagcgtca ctgcgaagtc gtgaacgcgg
     9121 attgacaaca gacccggaag agaatacaat gccgaataaa gtcgtaggtt ttgttatttt
     9181 tggaccaaaa tgtattcgat gcttcaaaaa attctaacta acccactgat gtcacatgga
     9241 ctactttgat gtttttatta cctttctgga catggacagt ataccgtaca tacattctca
     9301 atggaggcac agaaagctct cggactaaat ctaaaatatc ttaaactgtg ttctgaagat
     9361 gaacggaggt cttacgggct tggaacgaca tgagggcgag tcattaatga cattattttc
     9421 atttttgggt gaactaaccc tttaataaaa ctgacatttg cagccaattg cacactttat
     9481 aatgttaaat acttgttgat tttagaaata cagtagcatt tagtgacaat caaacattaa
     9541 ttttatttct gattatattg acaaaaacac aggactgtaa ggctgacatg acagggtgaa
     9601 ttgatttcat tacattagtt ttgatggtaa cacttttaca ataaggttcg ttagttaact
     9661 acattagtta acatgaacta ataatgaact gcacgtatac atgttaattt caacatttac
     9721 taatacttta tatcttgtta acattagtta atgcactgtg aactaacatg aacaaacaat
     9781 gaacatctgt atttctatta actaacgtta acaaagatta acagatacag taacaaatgt
     9841 attgctcatg gttatttaat gttagttaat acatgaacta atcaacctca ttgtaaagtg
     9901 ttaccgtttt gacaatacaa catgtttgga aaaaaatttc tcgcttggtt ttcttgtgtt
     9961 ttgcatgaat tcaaacacaa tgagagcacg gaatgataat gattattcaa agctgcaata
    10021 ggcgccaaat ttcccgtgga cctaacgtta ccaattacaa atgcatttta gcattttatt
    10081 acgtgagcct tatattgtca atcgcatgca ttagtgaaaa taggcctact acacatataa
    10141 aaggcgcgcc atcaacgtca aaacatgcac acccaaaana aaaaaaaaaa aaaaaaaaaa
    10201 aaaactatca gtaaaacaaa atacttaatg tacttacctc aatatgcttc cttaggtttg
    10261 atggcgaact tttgaaggcc gatatttctt tattaagcgg gagacagagg acacatttca
    10321 tcttgaatga atctttattt acaccactta aagaaaagaa ttcgcgaaga tacggccacg
    10381 ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac
    10441 atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa
    10501 ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca
    10561 tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta
    10621 aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg
    10681 aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag
    10741 tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta
    10801 ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac
    10861 tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac
    10921 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
    10981 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    11041 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    11101 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    11161 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    11221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    11281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    11341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    11401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    11461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    11521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    11581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    11641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    11701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    11761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    11821 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    11881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    11941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    12001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    12061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    12121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    12181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    12241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    12301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    12361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    12421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    12481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    12541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    12601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    12661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    12721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    12781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    12841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    12901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    12961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    13021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    13081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    13141 cccgaaaagt g
//
