LOCUS       sequence-203761-       12351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transgenesis, appears rather late, likely due to long RFP maturation
            time but is brighter than most other Ath5 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Icha J, Kunath C, Rocha-Martins M, Norden C
  TITLE     Independent modes of ganglion cell translocation ensure correct 
            lamination of the zebrafish retina.
  JOURNAL   J Cell Biol. 2016 Oct 24;215(2):259-275.
  PUBMED    27810916
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-10-24"; volume: "215(2)"; pages: "259-275"
COMMENT     Sequence Label: ath5:H2B-RFP
FEATURES             Location/Qualifiers
     source          1..12351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1346..1364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1389..1405
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1390..1406
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1440..1456)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6392..6408)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6445..6463)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    6463..6487
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6490..6867
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             6889..7563
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             6889..7563
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(7019..7037)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7287..7306
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7481..7504
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(7577..7601)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7606..7740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7630..7649)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7684..7703
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(9881..10068,10069..10080)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10114..10130)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(10167..10185)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10206..10222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10206..10222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10219..10241)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10230..10246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10254..10260,10261..10278,10279..10284))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10299..10320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10437..10454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10608..11196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10688..10707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11367..12158,12159..12227))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11990..12009
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12228..12332)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttggtaata cgactcacta tagggcgaat tgggtaccgg
     1381 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     1441 atccactagt tctagaattt aaatatttta aaaatgtata tatataataa atgtatatat
     1501 atataaatgt ataatgagca aaattctaag ttttatgcaa ataaaaaaag tattatggaa
     1561 aatgtattat attgcagttc taaattattt aagaaaacgt ggctgacttg ccaaaatgat
     1621 ggtaaactgt gcaactctgc ttccaagatg ttagaaacag acgtttttct tttagcctca
     1681 taatttaatg ctgttgcgat gttaccgtct cttcgctgta ctggttgtta ccaggttacg
     1741 gaaaaatata acatgctcac aacatccaat cagattaaat gaactgaaaa gcaatatggg
     1801 ttttaagaca tcacagagaa ggtaacattt aaaggcttaa aagcacaaac tgtttctcgc
     1861 ttctaagact aagtaagtaa gtttttgcac acaattaaca aatagttgca ggcaaattaa
     1921 actttagtga caaggcagta ctggcccgat caggccagta atgattttgt ctactgtcca
     1981 aagcatctct cacgctggcc ctgggtcatc aagcagttct tattgttgag ccctgcatta
     2041 taattatata caattttttt tttattattt aattttttta aattaaaatg ttatgtaatg
     2101 ttatgttaat aaatgtttaa ataagaatat taaaaaataa tttgatttaa tacaaataca
     2161 gggaagcacg ttttaaaaag taaataaatt ttgttaaatt ttatataagg aaaatatgtt
     2221 tatttctgta tttttctata cactgaacac aactaagaat ttctaaataa atatagacaa
     2281 atgctattag gcatgggatg ataaccagtt taaaggtcaa ggtttaaaaa cccccagaat
     2341 tatctgttat accgttccaa cgatatatgt aattttttta ttaacacttt taataaaaaa
     2401 ggaccctcat catcaaactg ttcttaaaat aaactatatt gtgctcaaag gtaaaaacag
     2461 ttcttgtttt ttacccagac atttaaaaaa agaatttatt ttagagcagt aatcacaata
     2521 ccgtccgtga taccgtgatt tttttttatc aaaggtaatc atacagtcac aatcttttac
     2581 cggcccatgc tatttatacg gcagtgtaaa ataaagtgta aattgtaacg cgttataaag
     2641 aattgcttct tttaaacaat gccacaaaaa ttatgaacat tttttttttg taaaaatttt
     2701 aaaccaaaaa taactaaaaa tttctccaaa tataaaacaa ttgagcaggg cacattgtaa
     2761 cacataacga aaactgcttc cattgttttt ttttatactt gaaaatcctt ttcatctttg
     2821 tcccacataa gtcataacat taacaataat aattgaatat agatttcttg tctaattgac
     2881 tgcccttaat aagacattgc ttcattgacc agttaagatt aaacataaca accagaaggg
     2941 aacaaaaagg ctcaaatagg catgaaatta caagcacctt gcttgaccta attacagtga
     3001 cgagcctcat ctcaccagat gccccaatct gacggtgggg gttcaagtct cctcatctat
     3061 ggaggtcact gtttacacaa tatgcctcac ttttctgaat gacctcattg gtcaatatgc
     3121 cagttctgtg agaatcgatc aaaaatagct ttaaagatta aagattttaa gttttcttct
     3181 cactttttta ggtagattat gtgttgaaat gaacacaaca gtttataccc atatctcaga
     3241 gatcatatcg atgatgaact ttaatgaatg ataagttatt atattatata ttaaatcata
     3301 ttatattata atatattata ttctatattc gttttgatag atgtagtgtt taaatgaaca
     3361 gtttatactc atctcaagga tcataaagaa gtgtcatatc atagaactgt aaatgacaat
     3421 gttatattat tttgatggac ctttagatat attctttggt tattatactg tatatttttg
     3481 gtagattatg tttacatcat agagctttaa atgactagtt ctattctagt ctatttgatt
     3541 tttttttttt taaatgacca caacagttta tactcaaata tcagtgatca taatgttata
     3601 tcaacatata aaatctatat tatgttatat tatattatat tatattatat tatattatat
     3661 tatattatat tttattttat attagacagc acccattctc agttttacat ctattatttg
     3721 actgtcagct tgacttaagt aggagtttga tcattagtcg tcactttcct cgagttcaga
     3781 ggattacgca ttcgattttt aagaccattt tctccttcgg aatgaacggc gctttatgct
     3841 ttaaatgaaa accagattta ctaaatcctg gattaagctg ttaaactgat aatagacctt
     3901 ctcactttcc cctcgtcaag accgagtaaa tctttaatct gctcaacact gtctcaaaaa
     3961 ttacaggagt agcttgtaac atatggcctt ttgcatagga gtccagacaa gcctatcatg
     4021 tgaacgagtg aaaaaacaag gccgtttaac atgtctggac caagcccttt aaacgggaca
     4081 agcagcagct ggtcagggat gccaatctgc aatcaatgaa aacaaactaa accagtggat
     4141 tgggtttata tgcccaattg aacagcagaa ttactcatct aatgcagatg agcgtatatg
     4201 gcttgatggc cacagtcagc cgggacatga attatgcaga agagatatag cgatttgtca
     4261 cttgtacaca aatcagatgt aataatgtat gtatttagtg cagtatttta tatagaacgc
     4321 tactttacga tttcaagtac ctgtaggaaa atatgttata gataattact atactataga
     4381 ggtgataatt cataatgcat aactttaaaa acattttgtt tcatagtata gcaattatat
     4441 atacagtata taagatagac tgcatatata tatatatata tatatattat atctgtgtgt
     4501 tattatatta taaccttagt ataacaataa tagtattttg gaaaggttta tgaatgagtt
     4561 tttttgccaa caggcttttt ttggaggggg tgaaactgtg actgtctgaa tctgctttgt
     4621 ttgattcaca ttgaacagat tgagatcgac agcttgtcga agacttctgt ctgtctcaat
     4681 tcctctgaga cactcaaatc aattatttag tgctgcccca caaatgtgtt ttatatggtt
     4741 ttgttattgg tttactatat attattaaat atatagaatg taatatgtaa tatagagaaa
     4801 tgtgctgatg atacagtaga tgaatactga cactatatta aaatattcta tataaaagta
     4861 taaaatttga acactgacgc tatattaaaa gattgcaaat gaccgtataa atgtttaaaa
     4921 tgtagaaact tcacatgtta ttttttctaa acaagcatgc tggttattac tttataatta
     4981 tgtaacaata taactgtact aatacagttc agtaattaac aagtaattca aaaatatttt
     5041 tattttaaat gtttatattt tatcattatt cttcaagcca ctaggttata ttagtttgtc
     5101 agcaaaaatg aacaaattag caccaacgct tataatttaa aatattaaat cttgaaaata
     5161 ttttaaagaa tgttacattt aatgaaaatt attttaaaaa gcataagaaa aattacacac
     5221 tcaaattgca tttaatatgc tttccgtgta aattacttat gtaaatttaa tttgatgcac
     5281 actaaaatgt ctcgtctatg acctatatac ataatgtatt atcatattgt aactcaaata
     5341 ttacatgtaa tattaatatt accttaaata tttatatatt acaatttaac attatgaata
     5401 acaaagacaa ctggagtatg tttgaccaaa acaaaacaac aatctttcta tttgtaaaag
     5461 ttaattttaa aaaaactgtt gcgatgtgta aaatatccat ttactttatt cagtaaaact
     5521 aatgtggatt ataatagtat agcataaata tataacaata tatcaattta atattaacat
     5581 caatataaaa ttgatgaatc tatctatgaa aaaatatgaa aaattcatta tttactcact
     5641 tattactaat tgcacacccg tatactattc ttttttcatt gaacacaaag aaagatatgt
     5701 tgaagtatgc tgaaaaccgg taaccattga cttcaacagt atttattttt ccaatggcag
     5761 ccagtggtta caggtttgca gctttcaacc aaatattttc ttttgtgatc agcagaagaa
     5821 agaaactcat aaaagtttgg aaccacttaa ataaaatagt gcataaatag tctgtaaatg
     5881 ttcatttttg tatgaactat cccttcaaat tatcgtttat attaaaagat tccagtaata
     5941 aatgtaaata atgagaccta caaccctgta aaacaaattg caaaatcaaa ctgactctat
     6001 acaggcaatt actcctgcaa acaatgttct catttaagaa atccaaagat gtcacggttg
     6061 cttaaagatg catgcacttt ttaattaggt aatggtcctt gccacccggc gctggttgtg
     6121 gccagatggt aagttatctc tggtttgtaa cgcctgtttt cagtgataga gcagccaaag
     6181 ttaattggaa tgtcattaaa gtttgagtta ctgggggttg gggctggact gcactagtcc
     6241 tgctgttgga gatgtgcgtt tcattaataa taatggatgt ttggatggag taataagggt
     6301 ggggctccac ctgctgccac ctgttaatac tctatataag catcgctcat ccttatggca
     6361 gaacagaagc gattaagaac catccggggg ggatccacta gttctagagc ggccgccacc
     6421 gcggtggagc tccagctttt gttcccttta gtgagggtta attcaagttt gtacaaaaaa
     6481 gcaggctgga tgccagagcc agcgaagtct gctcccgccc cgaaaaaggg ctccaagaag
     6541 gcggtgacta aggcgcagaa gaaaggcggc aagaagcgca agcgcagccg caaggagagc
     6601 tattccatct atgtgtacaa ggttctgaag caggtccacc ctgacaccgg catttcgtcc
     6661 aaggccatgg gcatcatgaa ttcgtttgtg aacgacattt tcgagcgcat cgcaggtgag
     6721 gcttcccgcc tggcgcatta caacaagcgc tcgaccatca cctccaggga gatccagacg
     6781 gccgtgcgcc tgctgctgcc tggggagttg gccaagcacg ccgtgtccga gggtactaag
     6841 gccatcacca agtacaccag cgctaaggat ccaccgggat ccaccggtat ggcctcctcc
     6901 gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
     6961 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     7021 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtcccctcag
     7081 ttccagtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     7141 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     7201 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     7261 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     7321 gcctccaccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagatgagg
     7381 ctgaagctga aggacggcgg ccactacgac gccgaggtca agaccaccta catggccaag
     7441 aagcccgtgc agctgcccgg cgcctacaag accgacatca agctggacat cacctcccac
     7501 aacgaggact acaccatcgt ggaacagtac gagcgcgccg agggccgcca ctccaccggc
     7561 gccgtgtaca tctaggaccc agctttcttg tacaaagttg atgtagatcc agacatgata
     7621 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     7681 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     7741 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     7801 taattcgcgg ccgcggcgcc aatgcattgg gccctcgagc atatgagatc tgtcccgatc
     7861 tttctcttct gtgctgtcaa tttagagtag tttttgaggt aatttgggtg cattctctgt
     7921 aaaaacaaca aataaatatt tatattgtat gcaataaatg taacaattac acatacactc
     7981 tctgattaca gcattaaagg gttagttcac ccaaaaatga aaagatgtca ttaatgactc
     8041 accctcatgt cgttccaagc ccgtaacacc tccgttcatc ttcggaacac agtttaagat
     8101 attttagatt tagtccgaga gctttctgtc cctccactga aaatgtatgt acggtatact
     8161 gtccatgtcc agaaaggtaa taaaaacatc aaagtagtcc atgtgacatc agtgggttag
     8221 gtagaattat ttgaagcatc gaaaatacat tttggtccaa aaataacaaa acctacgact
     8281 ttattcagca ttgtagcgtc actgcgaagt cgtgaacgcg gattgacaac agacccggaa
     8341 gagaatacaa tgccgaataa agtcgtaggt tttgttattt ttggaccaaa atgtattcga
     8401 tgcttcaaaa aattctaact aacccactga tgtcacatgg actactttga tgtttttatt
     8461 acctttctgg acatggacag tataccgtac atacattctc aatggaggca cagaaagctc
     8521 tcggactaaa tctaaaatat cttaaactgt gttctgaaga tgaacggagg tcttacgggc
     8581 ttggaacgac atgagggcga gtcattaatg acattatttt catttttggg tgaactaacc
     8641 ctttaataaa actgacattt gcagccaatt gcacacttta taatgttaaa tacttgttga
     8701 ttttagaaat acagtagcat ttagtgacaa tcaaacatta attttatttc tgattatatt
     8761 gacaaaaaca caggactgta aggctgacat gacagggtga attgatttca ttacattagt
     8821 tttgatggta acacttttac aataaggttc gttagttaac tacattagtt aacatgaact
     8881 aataatgaac tgcacgtata catgttaatt tcaacattta ctaatacttt atatcttgtt
     8941 aacattagtt aatgcactgt gaactaacat gaacaaacaa tgaacatctg tatttctatt
     9001 aactaacgtt aacaaagatt aacagataca gtaacaaatg tattgctcat ggttatttaa
     9061 tgttagttaa tacatgaact aatcaacctc attgtaaagt gttaccgttt tgacaataca
     9121 acatgtttgg aaaaaaattt ctcgcttggt tttcttgtgt tttgcatgaa ttcaaacaca
     9181 atgagagcac ggaatgataa tgattattca aagctgcaat aggcgccaaa tttcccgtgg
     9241 acctaacgtt accaattaca aatgcatttt agcattttat tacgtgagcc ttatattgtc
     9301 aatcgcatgc attagtgaaa ataggcctac tacacatata aaaggcgcgc catcaacgtc
     9361 aaaacatgca cacccaaaaa aaaaaaaaaa aaaaaaaaaa aaaactatca gtaaaacaaa
     9421 atacttaatg tacttacctc aatatgcttc cttaggtttg atggcgaact tttgaaggcc
     9481 gatatttctt tattaagcgg gagacagagg acacatttca tcttgaatga atctttattt
     9541 acaccactta aagaaaagaa ttcgcgaaga tacggccacg ggtgctcttg atcctgtggc
     9601 tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc
     9661 cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa
     9721 tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata
     9781 atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg
     9841 caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa
     9901 gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa
     9961 tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc
    10021 ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg
    10081 gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca
    10141 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
    10201 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    10261 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10321 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    10381 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    10441 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    10501 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    10561 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    10621 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    10681 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    10741 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    10801 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    10861 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    10921 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    10981 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11041 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    11101 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    11161 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    11221 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    11281 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    11341 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    11401 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    11461 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    11521 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    11581 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    11641 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    11701 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    11761 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    11821 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    11881 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    11941 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    12001 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12061 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    12121 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    12181 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    12241 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    12301 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt g
//
