LOCUS       sequence-203764-       13343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transgenesis, ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Icha J, Kunath C, Rocha-Martins M, Norden C
  TITLE     Independent modes of ganglion cell translocation ensure correct 
            lamination of the zebrafish retina.
  JOURNAL   J Cell Biol. 2016 Oct 24;215(2):259-275.
  PUBMED    27810916
REFERENCE   2  (bases 1 to 13343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-10-24"; volume: "215(2)"; pages: "259-275"
COMMENT     Sequence Label: bactin:GFP-DCX
FEATURES             Location/Qualifiers
     source          1..13343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6651..6675
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(6678..6680,6681..6683,6684..7394)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6678..6680,6681..6683,6684..7394)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6723..6744)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6984..7003)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7331..7352
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7422..7439
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7449..7466
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(8569..8593)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8598..8732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8622..8641)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8676..8695
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(10873..11060,11061..11072)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11106..11122)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11106..11122)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11159..11179)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11159..11177)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11198..11214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11198..11214)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11211..11233)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11222..11238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11246..11252,11253..11270,11271..11276))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11291..11312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11429..11446)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11600..12188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11680..11699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12359..13150,13151..13219))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12982..13001
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13220..13324)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttgaaaatt ccagtttgaa gaaacttttc aagactcacc
     1381 ttttcactgt tggtcttaat ttaggttgat tatttttgtc aatttaataa ttacattgta
     1441 ttgttcatat tttattgtct tattgtggtg tttttacagc ttttttattg ttctgctttg
     1501 ttactgtttg ccttgtatgc attgtagcgc tttgggtctg agaaaagcgc actataaata
     1561 aactgtatta ttattattag ggatggacat cacggtggcg cgatgggtgg cacaattacc
     1621 tcacagcaag taggttgctg tttagagccc cggctggatc agttggcagt tctgtgtgga
     1681 gtttgcatgt tctccctgtt caagtaggtt tcctcccggt gctctggttt cccctcacaa
     1741 gtccaaagac atgatgtata ggtgaatggg gtaagctaaa ttgaccatag tgagtgtgtg
     1801 tgtgtgtgtg aatgagagtg tataggtgtt tcccagtgtt aggttgcaac tggaaggaca
     1861 tctgctgtgt aaaacatatg caggataagt tggcggttca ttcccctatg gtgacccctg
     1921 gttaattaaa ggactaagtt gaaaatgaat gaatgaatga atgattagag ataaaccgat
     1981 accacttttt tacaaaccga tatgagtaca agtataatag tttctgttac tcgacaatac
     2041 tgagtaccga ttctgatact tcttggattt ggtttcaatg taattaattt gaaagaaggt
     2101 tttatttctt gcctgtcgca aggatgaaca caaaaatctt taccagaagg atattttaag
     2161 ctaagaatat acgcacgttg ttgacaaccc tgtatcgatt accgattaaa cgtggacatt
     2221 taaaggataa gttatgcttg cgccatcaca actttacaca ttacatgatc aaagatcata
     2281 ttgaatattc ggtttattcg tcagaagcct atagatttgt taaaagtgta ttttataaac
     2341 ggatggttta taaagactaa gctattaata tatcagacat tcattaatac atcaagacat
     2401 cgatgtcagg taggacaaca aatcacatcc atgtaatgaa atcttgttga gtggtattgg
     2461 tgcgtggttt cagcatttca tgtgcaatac gagtaagaat attttaacca agtatcgacc
     2521 caaaacctga tactggtatc ggtgcatccc ttattattat tattatcaaa aacacacttg
     2581 ttttatttat gtgggtgcca aacaacaaac tatgaaatgc agcatgacac gcccaaaaag
     2641 tgttcctttg agaacaactt cataaatatg tttcttatca ttatgcatga atggtgtact
     2701 gggagagctt tggtatcaga attgagataa gagactttgg cattctaaag gacaatgaca
     2761 ttatcacact gacagtcaac aacactttat tgctctggaa atcttatgtc atggggcaaa
     2821 gctgaggttc attaggacag taccttattt agagatgaat gggaaggctg tagtgggctg
     2881 ctatcaggga agggtggtgg tcctcggggg aactatggac tcacaaaagc acatctgctg
     2941 taacaagatc ccctgtgcaa tctacaaaag ttacaaagag aagcatggtt ggttaaaaaa
     3001 tagaattgac tggatatgct gaatgcattg tttgttgtgt gttacatcac attaaccaca
     3061 gattggtgct aaatatttgc ttatcttata aaaacctgtg tgtttataaa ggggggtgtg
     3121 gatggatgct tggcattctt tgaaaagatg ttgggggatg gaaggaggag accttattga
     3181 tgagtatcca gtttgcagat aacaagtttt tagacattca tactatgatg ctgtttatgt
     3241 ctgttttttc tcattttttc tatacacaaa cagctataaa acattcactt ttatatctga
     3301 tgctgtatat taaatttcgt tgtatttatt ctgtttgctc tgttgggtca tagctagggc
     3361 cagacagaat ctgctgacat tttttgcttt ttctgcagag aattttgtaa acaatctgtg
     3421 atttatgcga gattttttcg ggagtatcgt aactaaaaac ttactatatg aaataaaaaa
     3481 aaataatacc tttttaactt ttaataaata tatacaatgc aagtccacct agatccaatt
     3541 atttggtcga caaagcaagt ctctcacata atatatcttc taaaagacag aaaacattac
     3601 tttcagacca tattgtaatc aaatgaacac tttcatatta gtcaataata ttacggaaat
     3661 gtatttaaaa actgaataaa catatattta cacctttgtc tttatttcta gagtgctttt
     3721 acaatgtaga ttgtgtcaaa gcagcttaac ttagaggatt agtatagtaa attgaaactg
     3781 tgtcagtcca ttttttaaga gtttaagttc agttcagttt agttcagtgt gagttaattt
     3841 ttactgctga gagtccaaac actaaagagc aaatccaaat acacaagtca ataaataaac
     3901 tcaatgatgg gctaaagatc tggggaaatc tgcagatttc tgtgcgcaca gattctgtgt
     3961 gggcctagtc attacctata ctcaacgttt actctgagaa acttccatat tttagaggta
     4021 aaatgctcag aattgtaaaa agtcttgtca aaatacttgg aatttgccaa aaacaaaata
     4081 aaagtcacat cacctcattg tccttggtat cagcaagcac ttttaacaca atcagctgtt
     4141 tttgtttata actcccactg aaggccatgt gcattactcc caagttacac agtaaaaagt
     4201 cacatgtagt ttatcatgat cactgctgct caagcacagc tttatctgtt attcagtgtg
     4261 gcaatatctg aaatgtgtgc aaaatattat ttattgtagc tgatcaccaa cactttcagt
     4321 gttttttttt tctactttta atgagaatta tgttcttagg atttatcctt ataaacctct
     4381 ttaaaaagaa atgttatgtc tgcatagaca cgacatgaaa ttatgaacag aaaatttaac
     4441 tatttaagat caagacttga catataacat ttctaatgaa cattaaacct gaggtaggcc
     4501 cacttgcaaa aatgtaactg ctatcactca ctttatacat cattgtgtaa cttgtacaca
     4561 caaaaatagt gaagcaatgt ggcatggaat gcaggtccca ttcattcaaa gttattaaag
     4621 cactttctta aagtgcatta ctactgagct aagaccagct agttgctcat acaacatact
     4681 acaagtagtg tcatcctaat ttggataaaa aaaatgtcca ttttatacat ggaaaagtac
     4741 tcattagatc ccccattgtt tgtattacgg tatttcgtga acacaagagg taaatgacct
     4801 acagagctgc tgttgtgtta gattgaaaac acaacacagg atcatggagg catccatcat
     4861 tatgacgcat tctgccactt gtaactctgc acagactttt tgaaaagttt aattgagtct
     4921 tggcgttgtc gcagaaaaat acgaaaaatt ctgtgttaaa atttgcaaat aaaaattcca
     4981 gttttatagg aaatcgccat ggccttgttc tttaaccagt taagcttccc cttctttcac
     5041 tctcaagttg caagaagcaa gtgtagcaat gtgcacgcga cagccgggtg tgtgacgctg
     5101 gaccaatcag agcacagagc tccgaaagtt taccttttat ggctagagcc gggtatgtgc
     5161 cgtcatataa aagagcgcgc ccagcttttc agcctcactt tgagctcctc cacacgcagc
     5221 tagtgcggaa tatcatctgc ttgtaaccca ttctcttaag tcgacaaccc cccaaaccca
     5281 aggtgagttg gttttacagt ttttaagcgt ttaattatag tttctatcta ttaatgttaa
     5341 gtaaaatgtg taaatggata aatatgattt gatgaagtct ttagacagtt cataacggtc
     5401 tctttgtagt tatctactag gtttgattta taagtggtgg ttcttttgat aatctatgtt
     5461 acatttaata aatattggct gtatcttaac ttttttgtag cgttagtgga tgtggcaggt
     5521 gagaatgtcg gtaatttaac gtgaccagtc gtaggcacga cattgaacat tgaataggcc
     5581 ggtgtgaaat gggtgttaag tcttaatata aagttgtgtt ctggctaatc tctctttaac
     5641 agccataaat gtgattttta actacagttt tgagggtgct ggtgagcatg ttgcacactt
     5701 gatggatagc cggcatggga agttctttgt gcaggcagtg ctgcagcagg gtgtgaccta
     5761 ctttagctag ccggctaacc agctctcatc tgatgtaacg taaaccccat caatctaaca
     5821 tcggctcatt cgtgctttat aagatcgaag atcttgttat tagtaacggg tattggcatt
     5881 tagttttaac ttggttaatt ttctttttgt tatgtgttct atttaaatgt agcctgcagc
     5941 cccctctgtc tttaatgaag ctgaagatta acgttaacgt tagtgaagtg ttgtatccgc
     6001 tatgaaagat ttgaatttta gaggtgtggt ccactttaat aacggctgcg gagttctctg
     6061 gctgcacttc agccgctgtg actgtcgaag cagagcccat cagtttttca ggatctcggt
     6121 gatttttgag gctagactat gaactgaacc gactgagagt gaccgtccag aaatgcttta
     6181 gtgtgaagtc tccgctcacg aggagtccgt ggactgactg cagatctgtg acgcagtaac
     6241 acccggcggc agacacccgt tgaaatgcgg ttgtgtaatt gatcgcaggc gagggtggaa
     6301 gaggatgtga aacttcattt gtgtaaaatt tagggagtgg tcctggtgtg atgaatgtcg
     6361 aaatctgttc ctttttactg agccctacga ctctggctga gtgccacacc gccggcagcc
     6421 gcaaagcggc tcaaaccatt gccttttatg gtaataatga gagaatgcag agggacttcc
     6481 tttgtctggc atatctgagg cgcgcgctgt cactagcgcc caccagcggt cagactgtag
     6541 aatgcagagc aaaacaggaa gttgactcca gatggtcaca tgcttgctga aacgcctctc
     6601 cctggcagca gtgcaattct aacagtttcc ctttctttta cagttcagcc tcaagtttgt
     6661 acaaaaaagc aggctggatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     6721 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     6781 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6841 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     6901 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6961 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     7021 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     7081 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     7141 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     7201 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     7261 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     7321 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     7381 acgagctgta caagtccgga ctcagatcca tgggcagcag ccatcatcat catcatcaca
     7441 gcagcggcct ggtgccgcgc ggcagccata tggaacttga ttttggacac tttgacgaaa
     7501 gagataagac atccaggaac atgcgaggct cccggatgaa tgggttgcct agccccactc
     7561 acagcgccca ctgtagcttc taccgaacca gaaccttgca ggcactgagt aatgagaaga
     7621 aagccaagaa ggtacgtttc taccgcaatg gggaccgcta cttcaagggg attgtgtacg
     7681 ctgtgtcctc tgaccgtttt cgcagctttg acgccttgct ggctgacctg acgcgatctc
     7741 tgtccgacaa catcaacctg cctcagggag tgcgatacat ttacaccatt gatggatcca
     7801 ggaagatcgg aagcatggat gaactggagg aaggggaaag ctatgtctgt tcctcagaca
     7861 acttctttaa aaaggtggag tacaccaaga atgtcaatcc caactggtct gtcaacgtaa
     7921 aaacatctgc caatatgaaa gccccccagt ctttggctag cagcaacagt gcacaggcca
     7981 gggagaacaa ggactttgtg cgccccaagc tggttaccat catccgcagt ggggtgaagc
     8041 ctcggaaggc tgtgcgtgtg cttctgaaca agaagacagc ccactctttt gagcaagtcc
     8101 tcactgatat cacagaagcc atcaaactgg agaccggggt tgtcaaaaaa ctctacactc
     8161 tggatggaaa acaggtaact tgtctccatg atttctttgg tgatgatgat gtgtttattg
     8221 cctgtggtcc tgaaaaattt cgctatgctc aggatgattt ttctctggat gaaaatgaat
     8281 gccgagtcat gaagggaaac ccatcagcca cagctggccc aaaggcatcc ccaacacctc
     8341 agaagacttc agccaagagc cctggtccta tgcgccgaag caagtctcca gctgactcag
     8401 gtaacgacca agacgcaaac ggaacctcca gcagccagct ctctaccccc aagtctaagc
     8461 agtctcccat ctctacgccc accagtcctg gcagcctccg gaagcacaag gacctgtacc
     8521 tgcctctgtc cttggatgac tcggactcgc ttggtgattc catgtaagac ccagctttct
     8581 tgtacaaagt tgatgtagat ccagacatga taagatacat tgatgagttt ggacaaacca
     8641 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     8701 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     8761 ttcaggttca gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt
     8821 gggccctcga gcatatgaga tctgtcccga tctttctctt ctgtgctgtc aatttagagt
     8881 agtttttgag gtaatttggg tgcattctct gtaaaaacaa caaataaata tttatattgt
     8941 atgcaataaa tgtaacaatt acacatacac tctctgatta cagcattaaa gggttagttc
     9001 acccaaaaat gaaaagatgt cattaatgac tcaccctcat gtcgttccaa gcccgtaaca
     9061 cctccgttca tcttcggaac acagtttaag atattttaga tttagtccga gagctttctg
     9121 tccctccact gaaaatgtat gtacggtata ctgtccatgt ccagaaaggt aataaaaaca
     9181 tcaaagtagt ccatgtgaca tcagtgggtt aggtagaatt atttgaagca tcgaaaatac
     9241 attttggtcc aaaaataaca aaacctacga ctttattcag cattgtagcg tcactgcgaa
     9301 gtcgtgaacg cggattgaca acagacccgg aagagaatac aatgccgaat aaagtcgtag
     9361 gttttgttat ttttggacca aaatgtattc gatgcttcaa aaaattctaa ctaacccact
     9421 gatgtcacat ggactacttt gatgttttta ttacctttct ggacatggac agtataccgt
     9481 acatacattc tcaatggagg cacagaaagc tctcggacta aatctaaaat atcttaaact
     9541 gtgttctgaa gatgaacgga ggtcttacgg gcttggaacg acatgagggc gagtcattaa
     9601 tgacattatt ttcatttttg ggtgaactaa ccctttaata aaactgacat ttgcagccaa
     9661 ttgcacactt tataatgtta aatacttgtt gattttagaa atacagtagc atttagtgac
     9721 aatcaaacat taattttatt tctgattata ttgacaaaaa cacaggactg taaggctgac
     9781 atgacagggt gaattgattt cattacatta gttttgatgg taacactttt acaataaggt
     9841 tcgttagtta actacattag ttaacatgaa ctaataatga actgcacgta tacatgttaa
     9901 tttcaacatt tactaatact ttatatcttg ttaacattag ttaatgcact gtgaactaac
     9961 atgaacaaac aatgaacatc tgtatttcta ttaactaacg ttaacaaaga ttaacagata
    10021 cagtaacaaa tgtattgctc atggttattt aatgttagtt aatacatgaa ctaatcaacc
    10081 tcattgtaaa gtgttaccgt tttgacaata caacatgttt ggaaaaaaat ttctcgcttg
    10141 gttttcttgt gttttgcatg aattcaaaca caatgagagc acggaatgat aatgattatt
    10201 caaagctgca ataggcgcca aatttcccgt ggacctaacg ttaccaatta caaatgcatt
    10261 ttagcatttt attacgtgag ccttatattg tcaatcgcat gcattagtga aaataggcct
    10321 actacacata taaaaggcgc gccatcaacg tcaaaacatg cacacccaaa aaaaaaaaaa
    10381 aaaaaaaaaa aaaaaactat cagtaaaaca aaatacttaa tgtacttacc tcaatatgct
    10441 tccttaggtt tgatggcgaa cttttgaagg ccgatatttc tttattaagc gggagacaga
    10501 ggacacattt catcttgaat gaatctttat ttacaccact taaagaaaag aattcgcgaa
    10561 gatacggcca cgggtgctct tgatcctgtg gctgattttg gactgtgctg ctcgcagctg
    10621 ctgatgaatc acatacttcc tccattttct tccactgatt gactgttata atttccctaa
    10681 tttccaggtc aaggtgctgt gcattgtggt aatagatgtg acatgacgtc acttccaaag
    10741 gaccaatgaa catgtctgac caatttcata taatgtgaaa acgattttca taggcagaat
    10801 aaataacatt taaattaaac tgggcatcag cgcaattcaa ttggtttggt aatagcaagg
    10861 gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa taaaattgta
    10921 aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta aaagtattga
    10981 tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag tacagtaatc
    11041 aagtaaaatt actcaagtac tttacacctc tggttcttga ccccctacct tcagcaagcc
    11101 cagcagatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc
    11161 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    11221 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    11281 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    11341 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    11401 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    11461 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    11521 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    11581 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    11641 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    11701 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    11761 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    11821 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    11881 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    11941 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    12001 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    12061 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    12121 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    12181 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    12241 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    12301 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    12361 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    12421 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    12481 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    12541 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    12601 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    12661 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    12721 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    12781 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    12841 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    12901 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    12961 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    13021 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    13081 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    13141 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    13201 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    13261 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    13321 cgcgcacatt tccccgaaaa gtg
//
