LOCUS       sequence-203775-       13035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transgenesis, ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13035)
  AUTHORS   Daniel K, Icha J, Horenburg C, Muller D, Norden C, Mansfeld J
  TITLE     Conditional control of fluorescent protein degradation by an 
            auxin-dependent nanobody.
  JOURNAL   Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5.
  PUBMED    30120238
REFERENCE   2  (bases 1 to 13035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-08-17"; volume: "9(1):3297. doi"; pages: " 
            10.1038/s41467-018-05855-5"
COMMENT     Sequence Label: bactin:GFP-LAP2b
FEATURES             Location/Qualifiers
     source          1..13035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(1900..1911,1912..2074)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     2447..2463
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2483..2503
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7810..7834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7837..7846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7843..7845,7846..7848,7849..8559)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7843..7845,7846..7848,7849..8559)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7888..7909)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8149..8168)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8496..8517
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8560..8625
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(8639..8663)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9329..9349)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(9357..9373)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    9389..9523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9413..9432)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9467..9486
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(9962..10149,10150..10161)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10195..10211)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10195..10211)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10248..10268)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10248..10266)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10287..10303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10300..10322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10311..10327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10335..10341,10342..10359,10360..10365))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10380..10401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10518..10535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10689..11277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10769..10788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11448..12239,12240..12308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12071..12090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12309..12413)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12439..12894)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12576..12597)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12788..12807
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(13021..13035,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtacca
       61 aatagtagga attacccacc tgtacaagtg ctgaaaactt ggatgaataa gcccgtttgc
      121 cattttctac tgctatttta aatcttttct gtatttgtct gcatttgtct ttacccttga
      181 ataaatgttt tagttgtttt ttttcaatta tgactgtgtt taacagacat tattacaata
      241 atatgtaata gtagtacaca ctattattat gtaatagtac ttgttgactg tatttgagaa
      301 ctggaccgag tgagtgttac gtcacccatt caaaatgact tacttctggc tccaacaaaa
      361 tgaagttaat tcagttgcca tttttcactg tatggacatc gccgtgttgg agctagacgt
      421 tgccaataag caagaaagag ccgagatgcg tcgagtctga gtcaccgttc ctatggcaac
      481 ccctctaacc aatcagaagt aagcttgttg gaagtccaca gcctaccact tgaaagcggg
      541 ctgcacaaaa tctgtcaaac gttttgaacg ttggatgtga gagcacatac ttttattaag
      601 gcatctggtt ggtcagttta taatatcaac aacttgggct acagaaaaga aaagttatta
      661 cagaaattat gattaacaag tacatgttaa ataaagattt taatatgaat gccaccactg
      721 gagcattcat gccatttgga gcttcttcct gtttggatca ctagaaggag gaggtcactc
      781 attacagttc tcatatacag tcgttggttg gttggttggt tggtagattg attgattgat
      841 tgattgattg attgattgat tgattgattg attgattgat tgattgattg attgattgat
      901 tgattggtag tcaaaataag aaataatttc cacagattca ttacagaaat gattaaatgc
      961 atacataaaa aactgggggg gggggggata caacaacaca cttaagtcac atttgcctac
     1021 gtaaagaaaa gtaaagaaaa tcaatagcta tattttacat ctcctttttt tgctgtcttt
     1081 taattagcct tgttttgctg ttatcttgat tgaaactgta acttcttcac ctgcttcttt
     1141 tctttgtagg ttttgccagc ccagaaacag gcatggctgt gcaaggccag agcaccatgc
     1201 acaatgcgct gcatgtcttc atgaacggct caatgtcctc agtccagggc tcagccaacg
     1261 accccatctt cctccttcac catgctttca ttgacaggta acaaacacgt catgacatta
     1321 gactgcacag tttttgacaa agttcataca atctgttgtt tatagctgct acaattagtg
     1381 aagtttgtga atgtacttgg atgagcagcg aaagatcaat tgagatcaat tgttagagtt
     1441 tggttgccct gcagagcaaa gaacaaaaaa taatctggtg gctttactgc gtgaggttat
     1501 tattggtgga atagaaacac aaaacataat tgcatttatt tgtttaattt tttatcttat
     1561 cttaactttc atcttgcata tttgtttctt acatcatttc tagcatcttt gagcgctggc
     1621 taagaactca tcagcctccc cggtccatct acccacgtac caatgcacca attggccaca
     1681 atgacggcta ctacatggtg ccattccttc ctctttatag gaatggagac tacctcctgt
     1741 ccaacaatgc tcttggatac gagtacgcct acctgttgga cccaggtcat tgcacaacac
     1801 cagaaatgcc ctctgatctg caaaagacgt gaatatctgt tcagacaccc atatccactc
     1861 tgttccacac aggtcagagg tttgtccagg agttcttgac agaggtgtaa aaagtactca
     1921 aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa
     1981 aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac
     2041 ttgagtatta aggaagtaaa agtaaaagca agaaagaaaa ctagagattc ttgtttaagc
     2101 ttttaatctc aaaaaacatt aaatgaaatg catacaaggt tttatcctgc tttagaactg
     2161 tttgtattta attatcaaac tataagacag acaatctaat gccagtacac gctactcaaa
     2221 gttgtaaaac ctcagattta acttcagtag aagctgattc tcaaaattgt tagtgtcaag
     2281 cctagctctt ttggggctga aaagcaatcc tgcagtgctg aaaagcctct cacaggcagc
     2341 cgatgcggga agaggtgtat tagtcttgat agagaggctg caaatagcag gaaacgtgag
     2401 cagagactcc ctggtgtctg aaacacaggc cagatgggcc ctcgagcagg aaacagctat
     2461 gaccatgatt acgccaagct atcaactttg tatagaaaag ttgaaaattc cagtttgaag
     2521 aaacttttca agactcacct tttcactgtt ggtcttaatt taggttgatt atttttgtca
     2581 atttaataat tacattgtat tgttcatatt ttattgtctt attgtggtgt ttttacagct
     2641 tttttattgt tctgctttgt tactgtttgc cttgtatgca ttgtagcgct ttgggtctga
     2701 gaaaagcgca ctataaataa actgtattat tattattagg gatggacatc acggtggcgc
     2761 gatgggtggc acaattacct cacagcaagt aggttgctgt ttagagcccc ggctggatca
     2821 gttggcagtt ctgtgtggag tttgcatgtt ctccctgttc aagtaggttt cctcccggtg
     2881 ctctggtttc ccctcacaag tccaaagaca tgatgtatag gtgaatgggg taagctaaat
     2941 tgaccatagt gagtgtgtgt gtgtgtgtga atgagagtgt ataggtgttt cccagtgtta
     3001 ggttgcaact ggaaggacat ctgctgtgta aaacatatgc aggataagtt ggcggttcat
     3061 tcccctatgg tgacccctgg ttaattaaag gactaagttg aaaatgaatg aatgaatgaa
     3121 tgattagaga taaaccgata ccactttttt acaaaccgat atgagtacaa gtataatagt
     3181 ttctgttact cgacaatact gagtaccgat tctgatactt cttggatttg gtttcaatgt
     3241 aattaatttg aaagaaggtt ttatttcttg cctgtcgcaa ggatgaacac aaaaatcttt
     3301 accagaagga tattttaagc taagaatata cgcacgttgt tgacaaccct gtatcgatta
     3361 ccgattaaac gtggacattt aaaggataag ttatgcttgc gccatcacaa ctttacacat
     3421 tacatgatca aagatcatat tgaatattcg gtttattcgt cagaagccta tagatttgtt
     3481 aaaagtgtat tttataaacg gatggtttat aaagactaag ctattaatat atcagacatt
     3541 cattaataca tcaagacatc gatgtcaggt aggacaacaa atcacatcca tgtaatgaaa
     3601 tcttgttgag tggtattggt gcgtggtttc agcatttcat gtgcaatacg agtaagaata
     3661 ttttaaccaa gtatcgaccc aaaacctgat actggtatcg gtgcatccct tattattatt
     3721 attatcaaaa acacacttgt tttatttatg tgggtgccaa acaacaaact atgaaatgca
     3781 gcatgacacg cccaaaaagt gttcctttga gaacaacttc ataaatatgt ttcttatcat
     3841 tatgcatgaa tggtgtactg ggagagcttt ggtatcagaa ttgagataag agactttggc
     3901 attctaaagg acaatgacat tatcacactg acagtcaaca acactttatt gctctggaaa
     3961 tcttatgtca tggggcaaag ctgaggttca ttaggacagt accttattta gagatgaatg
     4021 ggaaggctgt agtgggctgc tatcagggaa gggtggtggt cctcggggga actatggact
     4081 cacaaaagca catctgctgt aacaagatcc cctgtgcaat ctacaaaagt tacaaagaga
     4141 agcatggttg gttaaaaaat agaattgact ggatatgctg aatgcattgt ttgttgtgtg
     4201 ttacatcaca ttaaccacag attggtgcta aatatttgct tatcttataa aaacctgtgt
     4261 gtttataaag gggggtgtgg atggatgctt ggcattcttt gaaaagatgt tgggggatgg
     4321 aaggaggaga ccttattgat gagtatccag tttgcagata acaagttttt agacattcat
     4381 actatgatgc tgtttatgtc tgttttttct cattttttct atacacaaac agctataaaa
     4441 cattcacttt tatatctgat gctgtatatt aaatttcgtt gtatttattc tgtttgctct
     4501 gttgggtcat agctagggcc agacagaatc tgctgacatt ttttgctttt tctgcagaga
     4561 attttgtaaa caatctgtga tttatgcgag attttttcgg gagtatcgta actaaaaact
     4621 tactatatga aataaaaaaa aataatacct ttttaacttt taataaatat atacaatgca
     4681 agtccaccta gatccaatta tttggtcgac aaagcaagtc tctcacataa tatatcttct
     4741 aaaagacaga aaacattact ttcagaccat attgtaatca aatgaacact ttcatattag
     4801 tcaataatat tacggaaatg tatttaaaaa ctgaataaac atatatttac acctttgtct
     4861 ttatttctag agtgctttta caatgtagat tgtgtcaaag cagcttaact tagaggatta
     4921 gtatagtaaa ttgaaactgt gtcagtccat tttttaagag tttaagttca gttcagttta
     4981 gttcagtgtg agttaatttt tactgctgag agtccaaaca ctaaagagca aatccaaata
     5041 cacaagtcaa taaataaact caatgatggg ctaaagatct ggggaaatct gcagatttct
     5101 gtgcgcacag attctgtgtg ggcctagtca ttacctatac tcaacgttta ctctgagaaa
     5161 cttccatatt ttagaggtaa aatgctcaga attgtaaaaa gtcttgtcaa aatacttgga
     5221 atttgccaaa aacaaaataa aagtcacatc acctcattgt ccttggtatc agcaagcact
     5281 tttaacacaa tcagctgttt ttgtttataa ctcccactga aggccatgtg cattactccc
     5341 aagttacaca gtaaaaagtc acatgtagtt tatcatgatc actgctgctc aagcacagct
     5401 ttatctgtta ttcagtgtgg caatatctga aatgtgtgca aaatattatt tattgtagct
     5461 gatcaccaac actttcagtg tttttttttt ctacttttaa tgagaattat gttcttagga
     5521 tttatcctta taaacctctt taaaaagaaa tgttatgtct gcatagacac gacatgaaat
     5581 tatgaacaga aaatttaact atttaagatc aagacttgac atataacatt tctaatgaac
     5641 attaaacctg aggtaggccc acttgcaaaa atgtaactgc tatcactcac tttatacatc
     5701 attgtgtaac ttgtacacac aaaaatagtg aagcaatgtg gcatggaatg caggtcccat
     5761 tcattcaaag ttattaaagc actttcttaa agtgcattac tactgagcta agaccagcta
     5821 gttgctcata caacatacta caagtagtgt catcctaatt tggataaaaa aaatgtccat
     5881 tttatacatg gaaaagtact cattagatcc cccattgttt gtattacggt atttcgtgaa
     5941 cacaagaggt aaatgaccta cagagctgct gttgtgttag attgaaaaca caacacagga
     6001 tcatggaggc atccatcatt atgacgcatt ctgccacttg taactctgca cagacttttt
     6061 gaaaagttta attgagtctt ggcgttgtcg cagaaaaata cgaaaaattc tgtgttaaaa
     6121 tttgcaaata aaaattccag ttttatagga aatcgccatg gccttgttct ttaaccagtt
     6181 aagcttcccc ttctttcact ctcaagttgc aagaagcaag tgtagcaatg tgcacgcgac
     6241 agccgggtgt gtgacgctgg accaatcaga gcacagagct ccgaaagttt accttttatg
     6301 gctagagccg ggtatgtgcc gtcatataaa agagcgcgcc cagcttttca gcctcacttt
     6361 gagctcctcc acacgcagct agtgcggaat atcatctgct tgtaacccat tctcttaagt
     6421 cgacaacccc ccaaacccaa ggtgagttgg ttttacagtt tttaagcgtt taattatagt
     6481 ttctatctat taatgttaag taaaatgtgt aaatggataa atatgatttg atgaagtctt
     6541 tagacagttc ataacggtct ctttgtagtt atctactagg tttgatttat aagtggtggt
     6601 tcttttgata atctatgtta catttaataa atattggctg tatcttaact tttttgtagc
     6661 gttagtggat gtggcaggtg agaatgtcgg taatttaacg tgaccagtcg taggcacgac
     6721 attgaacatt gaataggccg gtgtgaaatg ggtgttaagt cttaatataa agttgtgttc
     6781 tggctaatct ctctttaaca gccataaatg tgatttttaa ctacagtttt gagggtgctg
     6841 gtgagcatgt tgcacacttg atggatagcc ggcatgggaa gttctttgtg caggcagtgc
     6901 tgcagcaggg tgtgacctac tttagctagc cggctaacca gctctcatct gatgtaacgt
     6961 aaaccccatc aatctaacat cggctcattc gtgctttata agatcgaaga tcttgttatt
     7021 agtaacgggt attggcattt agttttaact tggttaattt tctttttgtt atgtgttcta
     7081 tttaaatgta gcctgcagcc ccctctgtct ttaatgaagc tgaagattaa cgttaacgtt
     7141 agtgaagtgt tgtatccgct atgaaagatt tgaattttag aggtgtggtc cactttaata
     7201 acggctgcgg agttctctgg ctgcacttca gccgctgtga ctgtcgaagc agagcccatc
     7261 agtttttcag gatctcggtg atttttgagg ctagactatg aactgaaccg actgagagtg
     7321 accgtccaga aatgctttag tgtgaagtct ccgctcacga ggagtccgtg gactgactgc
     7381 agatctgtga cgcagtaaca cccggcggca gacacccgtt gaaatgcggt tgtgtaattg
     7441 atcgcaggcg agggtggaag aggatgtgaa acttcatttg tgtaaaattt agggagtggt
     7501 cctggtgtga tgaatgtcga aatctgttcc tttttactga gccctacgac tctggctgag
     7561 tgccacaccg ccggcagccg caaagcggct caaaccattg ccttttatgg taataatgag
     7621 agaatgcaga gggacttcct ttgtctggca tatctgaggc gcgcgctgtc actagcgccc
     7681 accagcggtc agactgtaga atgcagagca aaacaggaag ttgactccag atggtcacat
     7741 gcttgctgaa acgcctctcc ctggcagcag tgcaattcta acagtttccc tttcttttac
     7801 agttcagcct caagtttgta caaaaaagca ggctgggcca ccatggtgag caagggcgag
     7861 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     7921 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     7981 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     8041 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     8101 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     8161 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     8221 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     8281 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     8341 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     8401 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     8461 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     8521 gccgccggga tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgagct
     8581 caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagacac
     8641 ccagctttct tgtacaaagt ggctctgtac aagtactcag atctcgagct caagcttgca
     8701 ttaactaggg agtccacgag agggtcgaga agaactccaa ggagaagggt ggaaccctca
     8761 cagcattttc gtgtagatgg tgcagtaatt tcagagagta ctcccatagc tgagactata
     8821 aaggcttcca gcaacgactc cttagtggcc aataggttga ctggaaattt caagcatgca
     8881 tcttctattc tgccaatcac tgaattctca gacataacca gaagaacacc aaagaaacca
     8941 ttgacaagag ctgaagtggg agaaaaaaca gaggaaagaa gagtagaaag ggatattctg
     9001 aaggaaatgt tcccgtatga agcctctact ccaaccggaa ttagtgctag ttgccgcaga
     9061 ccaatcaaag gtgctgccgg ccggccgctc gagctcagtg acttcaggat ggaagaatcg
     9121 ttctcatcta agtacgtccc gaagtacgtt cccttggcag acgtcaagtc ggaaaagaca
     9181 aagaagggac gctccgttcc catgtggata aaaatgctgc tgtttgctct cgtggcgggt
     9241 tttttgtttt tggtctatca agctatggaa accaaccaag gaaacccctt cactaatttt
     9301 cttcaagata ctaaaatatc caactgagca actttattat acatagttga taattcactg
     9361 gccgtcgttt tacggtacca tcgatgatga tccagacatg ataagataca ttgatgagtt
     9421 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     9481 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     9541 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     9601 ctacaaatgt ggtatggctg attatgatcc tctagatcag atctgcgaag atacggccac
     9661 gggtgctctt gatcctgtgg ctgattttgg actgtgctgc tcgcagctgc tgatgaatca
     9721 catacttcct ccattttctt ccactgattg actgttataa tttccctaat ttccaggtca
     9781 aggtgctgtg cattgtggta atagatgtga catgacgtca cttccaaagg accaatgaac
     9841 atgtctgacc aatttcatat aatgtgaaaa cgattttcat aggcagaata aataacattt
     9901 aaattaaact gggcatcagc gcaattcaat tggtttggta atagcaaggg aaaatagaat
     9961 gaagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa
    10021 gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat ttttaattgt
    10081 actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca agtaaaatta
    10141 ctcaagtact ttacacctct ggttcttgac cccctacctt cagcaagccc agcagatcca
    10201 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
    10261 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    10321 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    10381 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    10441 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    10501 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    10561 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    10621 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    10681 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    10741 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    10801 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    10861 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    10921 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    10981 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    11041 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    11101 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    11161 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    11221 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    11281 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggc
    11341 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    11401 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    11461 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    11521 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    11581 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    11641 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    11701 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    11761 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    11821 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    11881 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    11941 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    12001 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    12061 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    12121 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    12181 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    12241 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    12301 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    12361 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    12421 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
    12481 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
    12541 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
    12601 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
    12661 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
    12721 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
    12781 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
    12841 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
    12901 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    12961 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    13021 cccagtcacg acgtt
//
