LOCUS       sequence-203781-       10804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock inducible transgenesis, first coiled coil domain plus 
            some more amino acids, truncated before second coiled coil domain, 
            it's first 811 amino acids of dynactin subunit p150 glued, has 
            dominant negative effect on dynein function.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Taverna E, Mora-Bermudez F, Strzyz PJ, Florio M, Icha J, Haffner C, 
            Norden C, Wilsch-Brauninger M, Huttner WB
  TITLE     Non-canonical features of the Golgi apparatus in bipolar epithelial 
            neural stem cells.
  JOURNAL   Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206.
  PUBMED    26879757
REFERENCE   2  (bases 1 to 10804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:21206. doi"; pages: " 
            10.1038/srep21206"
COMMENT     Sequence Label: hsp70: mKate2 dynactin1-811
FEATURES             Location/Qualifiers
     source          1..10804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2864..2888
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2891..3586
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(6030..6054)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6059..6193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6083..6102)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6137..6156
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8334..8521,8522..8533)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8567..8583)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8567..8583)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8620..8640)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8620..8638)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8659..8675)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8659..8675)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8672..8694)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8683..8699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8707..8713,8714..8731,8732..8737))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8752..8773
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8890..8907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9061..9649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9141..9160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9820..10611,10612..10680))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10443..10462
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10681..10785)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttgcttcag gggtgtcgct tggttatttc caaaaatcaa
     1381 attaatttta ttaaactatt agaacgagca tgttttgtct atatgctaca gaagataaaa
     1441 aataatagga gttaacagtt ataaaacaac acactttgtt tctattgatt gttgaccaca
     1501 ctggggtctc attaagttag attaaagaca cactaactgg gtcaaaagca gcagattgat
     1561 ttcatagcac cagggtaaac tttctaacac ttttacggca atcatataca ttaaaattaa
     1621 atacagacca cgactgaaca aggaggatga tctccaatat taaacaaaga gacttgtgcc
     1681 tatttctctg agggtaaaca tgacctctca agttagcaag ttggttttaa cactacaaaa
     1741 atagttaaga ctgcaatccc agaataaagt attggtttta accaatcaat atagtacagt
     1801 aaacatccat ttgttttgtt gaaacgttaa acaaatctga ccaaagctat tagcttatat
     1861 aaaacaggtt tgccttctat gtagctgaaa acaccacagg cccgattttg ctactgtgta
     1921 aaacatttca gcaagatttt tttattgcat ttttttttac tgaatcgttc aaacatttta
     1981 tcattttagt ttgttcattc attgcaactg gaaaaacaac acatcacaca accgcacata
     2041 tttcagcaat aagtacaata aaacactcaa ataaaaaaaa cattttaaat ctctttgtat
     2101 ttttgaccgc tgtttcgcgt aatttcacgg taaaactctg gaaatctcca ctacattcct
     2161 ctcagcggct cctctcaatg acagctgaag aagtgacgcg gctgcctgct gtgttttgat
     2221 tggtcgaatt cactggaggc ttccagaaca gtgtagagtc tgaacgggtg cgcgctctgc
     2281 tgtatttaaa gggcgaaaga gagaccgcag agaaactcaa ccgaagagaa gcgacttgac
     2341 aaagaagaaa agagcagcct gacaggactt tttccccgac gaggtgttta ttcgctctat
     2401 ttaagaatct actgtaaggt aagtctcaat atattgtact ctattggcta atcagaatta
     2461 tatagagatt atatgtactt aatgtcaaaa aatcaacttt gtatatgtaa tctttttaca
     2521 tgtggactgc ctatgttcat cttattttag gtctactaga aaattatatt tcccgttttc
     2581 acaataagga tttttaaaaa aagcaatgaa cagacgggca tttactttat gttgctgaca
     2641 ttattttata tgagcataat aaccataaat actagcaaat gtcctaaatg aatttgtgtt
     2701 aatgttgtct acaaaagaaa attagcgttt tacttgtaca actaataata acttggttat
     2761 taagagaatt tcacttgttg actagaaaaa tcctttcata atgaaacaat tgcaccataa
     2821 attgtataaa tataaaatta attctaattg tttttttttc ctgccaagtt tgtacaaaaa
     2881 agcaggctgg atggtgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga
     2941 gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga
     3001 gggcacccag accatgagaa tcaaggcggt cgagggcggc cctctcccct tcgccttcga
     3061 catcctggct accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat
     3121 ccccgacttc tttaagcagt ccttccccga gggcttcaca tgggagagag tcaccacata
     3181 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     3241 ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa
     3301 aacactcggc tgggaggcct ccaccgagac cctgtacccc gctgacggcg gcctggaagg
     3361 cagagccgac atggccctga agctcgtggg cgggggccac ctgatctgca acttgaagac
     3421 cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga
     3481 cagaagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt
     3541 ggctgtggcc agatactgcg acctccctag caaactgggg cacagagata tcatggcaca
     3601 gagcaagagg cacgtgtaca gccggacgcc cagcggcagc aggatgagtg cggaggcaag
     3661 cgcccggcct ctgcgggtgg gctcccgtgt agaggtgatt ggaaaaggcc accgaggcac
     3721 tgtggcctat gttggagcca cactgtttgc cactggcaaa tgggtaggca tgattctgga
     3781 tgaagcaaag ggcaaaaatg atggaactgt tcaaggcagg aagtacttca cttgtgatga
     3841 agggcatggc atctttgtgc gccagtccca gatccaggta tttgaagatg gagcagatac
     3901 tacttcccca gagacacctg attcttctgc ttcaaaagtc ctcaaaagag agggaactga
     3961 tacaactgca aagactagca aactgcgggg actgaagcct aagaaggcac cgacagcccg
     4021 aaagaccaca actcggcgac ccaagcccac gcgcccagcc agtactgggg tggctggggc
     4081 cagtagctcc ctgggcccct ctggctcagc gtcagcaggt gagctgagca gcagtgagcc
     4141 cagcaccccg gctcagactc cgctggcagc acccatcatc cccacgccgg tcctcacctc
     4201 tcctggagca gtccccccgc ttccttcccc atccaaggag gaggagggac taagggctca
     4261 ggtgcgggac ctggaggaga aactagagac cctgagactg aaacgggcag aagacaaagc
     4321 aaagctaaaa gagctggaga aacacaaaat ccagctggag caggtgcagg aatggaagag
     4381 caaaatgcag gagcagcagg ccgacctgca gcggcgcctc aaggaggcga gaaaggaagc
     4441 caaggaggcg atggaggcaa aggaacgcta tatggaggag atggctgata ctgctgatgc
     4501 cattgagatg gccactttgg acaaggagat ggctgaagag cgggctgagt ccctgcagca
     4561 ggaggtggag gcactgaagg agcgggtgga cgagctcact actgacttag agatcctcaa
     4621 ggctgagatt gaagagaagg gctcagatgg cgctgcatcc agttatcagc tcaagcagct
     4681 tgaggagcag aatgcccgcc tgaaggatgc cctggtgagg atgcgggatc tttcttcctc
     4741 agagaagcag gagcatgtga agctccagaa gctcatggaa aagaagaacc aagagctgga
     4801 agttgtgagg caacagcggg agcgtctgca ggaggagcta agccaggcag agagcaccat
     4861 tgatgagctc aaggagcagg tggatgctgc tctgggtgct gaggagatgg tggagatgct
     4921 gacagatcgg aacctgaatc tggaagagaa agtgcgcgag ttgagggaga ctgtgggaga
     4981 cttggaagcg atgaatgaga tgaacgatga gctgcaggag aatgcacgtg agacagaact
     5041 ggagctgcgg gagcagctgg acatggcagg cgcgcgggtt cgtgaggccc agaagcgtgt
     5101 ggaggcagcc caggagacgg ttgcagacta ccagcagacc atcaagaagt accgccagct
     5161 gaccgcccat ctacaggatg tgaatcggga actgacgaac cagcaggaag catctgtgga
     5221 gaggcaacag cagccacctc cagagacctt tgacttcaaa atcaagtttg ctgagactaa
     5281 ggcccatgcc aaggcaattg agatggaatt gaggcagatg gaggtggccc aggccaatcg
     5341 acacatgtcc ctgctgacag ccttcatgcc tgacagcttc cttcggccag gtggggacca
     5401 tgactgcgtt ctggtgctgt tgctcatgcc tcgtctcatt tgcaaggcag agctgatccg
     5461 gaagcaggcc caggagaagt ttgaactaag tgagaactgt tcagagcggc ctgggctgcg
     5521 aggagctgct ggggagcaac tcagctttgc tgctggactg gtgtactcgc tgagcctgct
     5581 gcaggccacg ctacaccgct atgagcatgc cctctctcag tgcagtgtgg atgtgtataa
     5641 gaaagtgggc agcctgtacc ctgagatgag tgcccatgag cgctccttgg atttcctcat
     5701 tgaactgctg cacaaggatc agctggatga gactgtcaat gtggagcctc tcaccaaggc
     5761 catcaagtac tatcagcatc tgtacagcat ccaccttgcc gaacagcctg aggactgtac
     5821 tatgcagctg gctgaccaca ttaagttcac gcagagtgct ctggactgca tgagtgtgga
     5881 ggtaggacgg ctgcgtgcct tcttgcaggg tgggcaggag gctacagata ttgccctcct
     5941 gctccgggat ctggaaactt catgcagtga catccgccag ttctgcaaga agatccgaag
     6001 gcgaatgcca gggacagatg ctccttaaga cccagctttc ttgtacaaag ttgatgtaga
     6061 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     6121 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     6181 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     6241 gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggccctcg agcatatgag
     6301 atctgtcccg atctttctct tctgtgctgt caatttagag tagtttttga ggtaatttgg
     6361 gtgcattctc tgtaaaaaca acaaataaat atttatattg tatgcaataa atgtaacaat
     6421 tacacataca ctctctgatt acagcattaa agggttagtt cacccaaaaa tgaaaagatg
     6481 tcattaatga ctcaccctca tgtcgttcca agcccgtaac acctccgttc atcttcggaa
     6541 cacagtttaa gatattttag atttagtccg agagctttct gtccctccac tgaaaatgta
     6601 tgtacggtat actgtccatg tccagaaagg taataaaaac atcaaagtag tccatgtgac
     6661 atcagtgggt taggtagaat tatttgaagc atcgaaaata cattttggtc caaaaataac
     6721 aaaacctacg actttattca gcattgtagc gtcactgcga agtcgtgaac gcggattgac
     6781 aacagacccg gaagagaata caatgccgaa taaagtcgta ggttttgtta tttttggacc
     6841 aaaatgtatt cgatgcttca aaaaattcta actaacccac tgatgtcaca tggactactt
     6901 tgatgttttt attacctttc tggacatgga cagtataccg tacatacatt ctcaatggag
     6961 gcacagaaag ctctcggact aaatctaaaa tatcttaaac tgtgttctga agatgaacgg
     7021 aggtcttacg ggcttggaac gacatgaggg cgagtcatta atgacattat tttcattttt
     7081 gggtgaacta accctttaat aaaactgaca tttgcagcca attgcacact ttataatgtt
     7141 aaatacttgt tgattttaga aatacagtag catttagtga caatcaaaca ttaattttat
     7201 ttctgattat attgacaaaa acacaggact gtaaggctga catgacaggg tgaattgatt
     7261 tcattacatt agttttgatg gtaacacttt tacaataagg ttcgttagtt aactacatta
     7321 gttaacatga actaataatg aactgcacgt atacatgtta atttcaacat ttactaatac
     7381 tttatatctt gttaacatta gttaatgcac tgtgaactaa catgaacaaa caatgaacat
     7441 ctgtatttct attaactaac gttaacaaag attaacagat acagtaacaa atgtattgct
     7501 catggttatt taatgttagt taatacatga actaatcaac ctcattgtaa agtgttaccg
     7561 ttttgacaat acaacatgtt tggaaaaaaa tttctcgctt ggttttcttg tgttttgcat
     7621 gaattcaaac acaatgagag cacggaatga taatgattat tcaaagctgc aataggcgcc
     7681 aaatttcccg tggacctaac gttaccaatt acaaatgcat tttagcattt tattacgtga
     7741 gccttatatt gtcaatcgca tgcattagtg aaaataggcc tactacacat ataaaaggcg
     7801 cgccatcaac gtcaaaacat gcacacccna aaaaaaaaaa aaaaaaaaaa aaaaaaacta
     7861 tcagtaaaac aaaatactta atgtacttac ctcaatatgc ttccttaggt ttgatggcga
     7921 acttttgaag gccgatattt ctttattaag cgggagacag aggacacatt tcatcttgaa
     7981 tgaatcttta tttacaccac ttaaagaaaa gaattcgcga agatacggcc acgggtgctc
     8041 ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc
     8101 ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg
     8161 tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga acatgtctga
     8221 ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat ttaaattaaa
     8281 ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat
     8341 ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt
     8401 ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt
     8461 aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta
     8521 ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc cactagttct
     8581 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     8641 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8701 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8761 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8881 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8941 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9001 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9061 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9781 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9841 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9901 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9961 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10021 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10081 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10141 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10201 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10261 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10321 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10381 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10441 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10501 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10561 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10621 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10681 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10741 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10801 agtg
//
