LOCUS       sequence-20380-e         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GPD-ccdB
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(20..144)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(152..168)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(152..168)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(174..437)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(257..280)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
       61 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      121 ttcagctttt ttgtacaaac ttgtgatggg ggatccacta gttctagaat ccgtcgaaac
      181 taagttctgg tgttttaaaa ctaaaaaaaa gactaactat aaaagtagaa tttaagaagt
      241 ttaagaaata gatttacaga attacaatca atacctaccg tctttatata cttattagtc
      301 aagtagggga ataatttcag ggaactggtt tcaacctttt ttttcagctt tttccaaatc
      361 agagagagca gaaggtaata gaaggtgtaa gaaaatgaga tagatacatg cgtgggtcaa
      421 ttgccttgtg tcatcattta ctccaggcag gttgcatcac tccattgagg ttgtgcccgt
      481 tttttgcctg tttgtgcccc tgttctctgt agttgcgcta agagaatgga cctatgaact
      541 gatggttggt gaagaaacaa tattttggtg ctgggattct ttttttttct ggat
//
