LOCUS       sequence-20381-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GPD-ccdB
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(115..239)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(272..288)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(273..289)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      291..538
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(307..325)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(614..630)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(614..630)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaagagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
       61 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
      121 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
      181 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg
      241 atgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag tcatgtaatt
      301 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg
      361 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
      421 aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa
      481 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg
      541 ccgtcgaggg ggggcccggt accagctttt gttcccttta atgagggtta atttcgagct
      601 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatcccctc acaattccac
      661 aca
//
