LOCUS       sequence-203830-       11364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g03090 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 11364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g03090-bio-His
FEATURES             Location/Qualifiers
     source          1..11364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6528..6545
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6597..6652
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6651..6670)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8888..8906)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8974..9078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9079..9147,9148..9939)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9297..9316)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10110..10698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10599..10618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10852..10869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10986..11007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11022..11027,11028..11045,11046..11052)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11060..11076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11065..11087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11084..11100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11084..11100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 agcctaccaa gagcatcagc agcttcaccg ccaacgactt ctacgtgttc aagaacgcct
     1261 gggccaccag caccttcatc gacagcgacg gcaacctgga agagttcagc cccctgagag
     1321 tgatccagcc tggccccagc ttctggtgca gcagcggcag ccacagcgcc agcgacatcg
     1381 tgtcctggac cggcgagttc gccaagcagc ccctgatcga gacactgacc atcgactgga
     1441 cctacgcccc caaagagttc cagatcagcg cctccgccga cggcatcaac tacgccagcc
     1501 tgatgccctt ccagcccatc ctggcccaga agcccagctt tacccagttc gtgaagctgc
     1561 ccgtgccttt cctgagcaaa gaactgaaga tcagcatgaa gaacccccag gacaacttct
     1621 tcggcatcag ctccgtgaag atccgggctg ccggcaaccc cctgttcatg ctgaagtccg
     1681 gcatcacctg ttctggcggc gagctgtgcc tggtcaacaa gaacaacaag cccctgctga
     1741 tcaactgcct ggaagccatt gccatctcca gcggcattga gctgtggcgg ttcaacagca
     1801 accagcagat cgtgtctatc tcccccctgc ccgacaccgg cagcatcgac ctggccgatc
     1861 tgagcgccaa gagcgagagc gtggacaagt gcctggccgt gcccctgaac tccgccaaca
     1921 acgccaatag cgccgccatc ggccagatct tcgaggtgtt catggccaac tgcgacgacc
     1981 tgctggacca ggacgacgcc ttcagccggt ggaacttcga gagcaactcc cagatctccc
     2041 tgctgtcccc ccagcctctg tgtctgagcc aggtggacat cttcccaacc cagtccggcg
     2101 agagcctgac cggcaatgtg aatctggccg gcctggtgga agtggaaatc aagagcctgc
     2161 acaccaaggg ccacgagccc aagtttgcca tcgataacaa gctggaaacc tactgggcca
     2221 gccctctgct gcagcccaac gacatcaaca aggccatcac cgtggaactg ctgctgaaga
     2281 atccctacct gattgacagc atcgtgatct actgggagta ccccgccttt agctacctgt
     2341 ttaagacctc cctggacaac atcaattaca acaacggccc ctacaacgcc gctaacggcg
     2401 ccaccaagac cgtggacatg tttggcatcg aggccaagta catccagctg accctgctgc
     2461 accctcacat taactacaaa gtgaagaaca atcacatcta cggcatccgc gagatccaga
     2521 ttatgaccta tgccaccaac acagtggtgg aaaactgcaa gatcgccgcc aactctgccg
     2581 acgcccggga caagtacttc gtggactacg tgacccagta cgaccccaat atcgccaagt
     2641 ctatcaccac actggaagct aatctggtgg acttcagcaa ctacatcttt gagaagtccg
     2701 actacctgaa cgagaagctg tcctacctgc acgatgccct ggaacagtcc aaagaattca
     2761 gcgacaccct gggcgatatc gacaagttcc tgaatgaagt gcggaaagag agccccttcg
     2821 acacctacga caagagctac atcggcgagt ccaagaacct gcccgccgac agctgccaga
     2881 ttgtgctgac ccacaacaaa cacgccaaaa gcggcttcta ctggctgaac cctgcctgct
     2941 cccccttcgc ctaccgggcc tactgccact tttaccaggc ccagagcaag ctgatcttta
     3001 tcgagaaggc cgagggcaac attgccaacc agaacaccgc tatcgcccgg acctgcgcca
     3061 aatacggcct ggaacctctg atcctgagcg ataagctgga ttacgacgcc ctggtggcca
     3121 gcgtgatcgc cttcggcgtg accgacttca tccccctggc caaggactac cagtgccacg
     3181 gctacaagtg ctccggcaat ttctacgacc tgtttcaggg aatcgagaac gtggccaacc
     3241 tgctgaaatt ccacagcaac accgcccctg gcgagtttga cgatctggct ggcttcacat
     3301 acgccgacca gatcgtctac aagaagtcct ccaacaccaa tattgcctac gtcgtgtgca
     3361 ccgacaacgg ctataacctc gacaatttcg agaaactcag ctgtgacgac gtgctgatcg
     3421 gcaacgaaaa gctgctggga gagctgaata ccgccatcta cgtggaatgc cccaccggct
     3481 gccagcacag caagatgccc atctatggca gcaattacgc cttctccgag acaagcagca
     3541 tctgcaaggc cgccattgag agcggcgtga tctccgacgg cggcagattc gtgatcacca
     3601 tcgagggccc tctgagcatg gacagccaga ataccaacaa catcgatacc ggggccaaga
     3661 ccaccggcga cggaaacgac gccaacacaa acctgtacgg ccggctgagc ttccggatca
     3721 accgggccac aaagaagtgc cccatcgaca tcctgaccga gtctaagcac acatacaaga
     3781 acgagcacgt gcacaagcag atcacccacg agctgctgca taacagcgat ctgttctttg
     3841 gcgccgagat taagctgcct gcctccgagt acgccaatga agaggtggac ccctggactg
     3901 agaaactgag caagttctcc gtggatcaga tcgacaccgt gtacggaatc gacccactgc
     3961 tggccaaaca cagcgtggaa gaggccatta tcacaatcga taagttcagc aagaaattca
     4021 agaaggtggc acggcggagc cggaagctgt tcatcaaggc cagcaacgtg ctggacgagc
     4081 tggaacggct gcacaatgat ctggaagtga ttcacagcga gagaagcgtg aaactgggaa
     4141 agatcgactc cagcaaagag gacaagggct ttgacctgga aaacaccccc atcttcaccg
     4201 agttcaaact ggacaccagc ggaatcagcg tggccgataa gtccctgaac gtggtgttcg
     4261 gcaccagcga cgagtggcgg atcgtgaaca ccagatacca cagcatcaac gactatctgg
     4321 cccactaccc cacaagcaat accaagcacc acaaccccgt gtccgacaac ccccccaacc
     4381 tggcaatcgc cctgatcaag tcccacaagt tcttcaactt caagctcatc ttcagcatca
     4441 ccagcctcga cgataagatc tgcggcatca tcttccgcta ccacgataac ttcaactact
     4501 acaagctcct gttcgacact ctgaacggcg agacacggct ggaaattgtg tccaacggcc
     4561 ggaccagccc actggccacc gtgaaaaatg gcggcctgac ccccgagcgg tggaccaaag
     4621 tgacactgtc cgctgacggc ccactgatca cagtggacct gggccggcat aacaccaaca
     4681 tcctgtctgt gctggatgag acaatctcct ccggctccat cggcttttac agcaccgggg
     4741 acaccttcta ctctaacctg gctgtgtccc ctctggactg cacccaggcc gagcaggtca
     4801 aaagcctgga catcaagtgt agcaacttcg tcgaggaatt tctgggcgac ccctacgata
     4861 cctatgatat cggcggcttc agcgtcaaga ccaacaatga gaactttgtg ggctggtctt
     4921 acagcggcaa catgctggtc cagaagagtc ctctggccat tctggaatct atcgctctgc
     4981 tgtctcacaa ccggaagtgc aagctgggcg tgttcagctt cctcgtgtgg cccgagagaa
     5041 agatccacgc cacccggatc ccttgcgtgg gcgccgtgtt cttctacaag gataacaaca
     5101 acttcggcta cctgagcctc cagaaggact ctatccagct cgtgggccgg aaggacggca
     5161 ccgagagcac agtgaaaaag gtcgagctga agggcttcac cttccacaag tggcacaacg
     5221 tgttcgtcaa gctcgccaca gacaacaatg tcaacgtcgc catcaatgac atcggcatcg
     5281 atggcaagat ggaaaagatt gccgtgatcg aggggaagtt cgacggcttt accgacggga
     5341 accggatcgg cctgtttgtg aacaatatcg ctcgggtgct gttcaaggac attcagctgc
     5401 acgacgagca gagcaccagc tccaatgtgg ccctgctcca gcagaacgag gtgtccaccg
     5461 gcactgacaa caagatctgg gacgagtgtc tggcaggcgg ctactacgag cggctggtgc
     5521 tgtgcaaaga gatccggatc gactgcaaca atttctgcgt gaactgttgc aaacaccaca
     5581 caagcctgct cagcaagcag agctacgacg aatgtctgat cgattgccag aacatccaga
     5641 cccccagcaa tgacaagaag gcctttgata agtatatcca gagctgttcc ccactgccct
     5701 acgggtataa caaaccccac tttaagcact gtcagaacga caacgagtgc atcatcaacg
     5761 cctgcatcct gtgctgcgag tccggaatca acaccgagga agaacactat ctgcagaagt
     5821 gctaccagaa ctgcgctaag agcgccctcc tgctggctaa gagacagttc gagctgggcg
     5881 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     5941 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     6001 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     6061 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     6121 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     6181 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     6241 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     6301 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     6361 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     6421 tatccaaagg gttgaattcc ggcagcctgc accacatcct ggacgcccag aagatgctgt
     6481 ggaaccacag ggacaggaac ctgcctcctc tggcccctct gggcccccac caccatcacc
     6541 accattgagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     6601 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     6661 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     6721 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     6781 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     6841 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     6901 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     6961 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     7021 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     7081 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     7141 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     7201 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     7261 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     7321 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     7381 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     7441 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     7501 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     7561 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     7621 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     7681 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     7741 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     7801 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     7861 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     7921 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     7981 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     8041 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     8101 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     8161 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     8221 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     8281 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     8341 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     8401 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     8461 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     8521 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     8581 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     8641 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     8701 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     8761 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     8821 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     8881 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     8941 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9001 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     9061 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     9121 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     9181 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9241 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9301 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     9361 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     9421 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     9481 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     9541 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     9601 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     9661 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     9721 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     9781 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     9841 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9901 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9961 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    10021 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    10081 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    10141 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    10201 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    10261 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    10321 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    10381 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    10441 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    10501 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10561 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    10621 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    10681 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    10741 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    10801 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    10861 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    10921 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    10981 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    11041 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    11101 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
    11161 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
    11221 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
    11281 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
    11341 ttggctcatg tccaatatga ccgc
//
