LOCUS       sequence-20387-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG306GPD-ccdB
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(116..240)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(273..289)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(274..290)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      292..539
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(308..326)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(576..594)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(615..631)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(615..631)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(628..650)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    639..655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(663..669,670..687,688..693))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggaagagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
       61 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag
      121 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
      181 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt
      241 gatgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gtcatgtaat
      301 tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag
      361 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa
      421 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta
      481 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg
      541 gccgtcgagg gggggcccgg taccagcttt tgttcccttt agtgagggtt aatttcgagc
      601 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      661 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaa
//
