LOCUS       sequence-20388-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GAL-ccdB
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..169)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(177..193)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(177..193)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(208..649)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(214..237)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    532..649
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(673..693)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(673..691)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(712..728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(712..728)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(725..747)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    736..752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cggaagcata aagtgtaaag cctgggggtg cctaatgcgg ccgccatagt gactggatat
       61 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
      121 tatttatatc attttacgtt tctcgttcag cttttttgta caaacttgtg atgggggatc
      181 cactagttct agaatccggg gttttttctc cttgacgtta aagtatagag gtatattaac
      241 aattttttgt tgatactttt attacatttg aataagaagt aatacaaacc gaaaatgttg
      301 aaagtattag ttaaagtggt tatgcagttt ttgcatttat atatctgtta atagatcaaa
      361 aatcatcgct tcgctgatta attaccccag aaataaggct aaaaaactaa tcgcattatc
      421 atcctatggt tgttaatttg attcgttcat ttgaaggttt gtggggccag gttactgcca
      481 atttttcctc ttcataacca taaaagctag tattgtagaa tctttattgt tcggagcagt
      541 gcggcgcgag gcacatctgc gtttcaggaa cgcgaccggt gaagacgagg acgcacggag
      601 gagagtcttc cttcggaggg ctgtcacccg ctcggcggct tctaatccgt actagagctc
      661 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
      721 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag cc
//
